An Analysis of the Micropropagation and Peculiarities of Eggplant (Solanum melongena L.) Cultivation Technology


This review is devoted to biotechnological methods and approaches used in eggplant selection. Using in-vitro techniques, micropropagation makes it possible to obtain Solanum melongena L. plants identical to the original ones. This article discusses various aspects of eggplant microcloning; draws conclusions about the successful induction of shoots, which is an important stage required for the regeneration of the callus; and describes the methodological issues of origin, explant treatment, cultivation features, composition of nutrient media, growth regulators, growing conditions and rooting algorithms, and adaptation of the resulting regenerant plants. The microclonal reproduction of Solanum melongena L. by organogenesis from cotyledon and hypocotyl explants is described. After explants were introduced into an in-vitro culture, significant differences in the nature of growth and development of the tissues in the nutrient media were observed on the 6-8th day. A comparative analysis of the stimulating effect of growth regulators revealed that thidiazuron with a concentration of 0.02 mg/l had the highest efficiency; its effect on the regenerative capacity of the explant depended on the concentration in the medium. The dependence of all stages of microclonal reproduction on the genotype is shown.

Keywords: Solanum melongena L., eggplant, nightshade, microcloning, biotechnology

[1] Verba, V. M., et al. (2010). Clonal Micropropagation of Eggplant by Organogenesis. Bulletin of Russian Agricultural Science, vol. 6, pp. 57-59.

[2] Mamedov, M. I., Pyshnaya, O. N. and Verba, V. M. (2009). Cultural Eggplant-Origin, Selection Problems and Genetic Sources. Vegetables of Russia, vol. 4, pp. 27-33.

[3] Vavilov, N. I. (1951). The Origin, Variation, Immunity and Breeding of Cultivated Plants. Leningadskiy Ovoshnoy Zhurnal, vol. 72, issue 6, p. 482.

[4] Pivovarov, V. F., Shmykova, N. A. and Suprunova, T. P. (2011). Biotechnological Methods in the Selection of Vegetable Crops. Vegetables of Russia, vol. 3, pp. 10-17.

[5] Matsubara, K., et al. (2005). Antiangiogenic Activity of Nasunin, an Antioxidant Anthocyanin, in Eggplant Peels. Journal of Agricultural and Food Chemistry, vol. 53, issue 16, pp. 6272-6275.

[6] Dubovitsky, A. A. and Klimentova, E. A. (2014). Problems and Prospects for the Development of Vegetable Growing. Technologies of Food and Processing Industry of the Agro-Industrial Complex - Healthy Food Products, vol. 3. pp. 17-32.

[7] Demenko, V. I. (2005). Problems and Possibilities of Microclonal Reproduction of Garden Plants. News of the Timiryazev Agricultural Academy, vol. 2. pp. 18-23.

[8] Acciarri, N., et al. (2002). Genetically Modified Parthenocarpic Eggplants: Improved Fruit Productivity Under Both Greenhouse and Open Field Cultivation. BMC Biotechnology, vol. 2, issue 1, p. 4.

[9] Toppino, L., et al. (2016). Mapping Quantitative Trait Loci Affecting Biochemical and Morphological Fruit Properties in Eggplant (Solanum melongena L.). Frontiers in Plant Science, vol. 7, p. 256.

[10] Calvo-Asensio, I., Prohens, J. and Gisbert, C. (2014). Vigor for In Vitro Culture Traits in S. Melongena × S. Aethiopicum Hybrids with Potential as Rootstocks for Eggplant. The Scientific World Journal, vol. 2014. pp. 1-7.

[11] Ayal, A. A., Sayed, M. A. E. and Pavlikov, R. E. (2017). Influence of Cultivation Conditions on Clonal Micropropagation of Ashwagandha (Withania somnifera L.). News of the Timiryazev Agricultural Academy, vol. 3. pp. 32-38.

[12] Matushkina, O. V. and Pronina, I. N. (2010). Features of the Effect of Exogenous Cytokinins and their Derivatives on the Regeneration of Apple and Pear in Vitro. Achievements of Science and Technology of Agro-Industrial Complex, vol. 8. pp. 1-12.

[13] Ualieva, D. A. and Bagasheva, S. S. (2018). Clonal Micropropagation of Valuable Potato Varieties. Scientific Community of Students of the XXI century. Natural Sciences, vol. 12, p. 19.

[14] Murashige, T. and Skoog, F. A. (1962). Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, vol. 15, issue 3, pp. 473-497.

[15] Kataeva, N. V. and Butenko, R. G. (1983). Clonal Micropropagation of Plants. Science, vol. 1302, pp. 1402-1408.

[16] Foo, P. C., et al. (2018). Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin. Tropical Life Sciences Research, vol. 29, issue 2, p. 119.

[17] Shmykova, N. A., Suprunova, T. P. and Pivovarov, V. F. (2015). Biotechnological and Molecular Genetic Methods in the Selection of Vegetable Crops (To the 95th Anniversary of VNIISSOK). Agricultural Biology, vol. 5. pp, 131-140.

[18] Savinova, N. I. (1989). Low-Volume Hydroponics: What Hinders its Implementation. Potatoes and Vegetables, vol. 6, p. 22.

[19] Mallaya, N. P. and Ravishankar, G. A. (2013). In Vitro Propagation and Genetic Fidelity Study of Plant Regenerated from Inverted Hypocotyl Explants of Eggplant (Solanum Melongena L.) Cv. Arka Shirish. 3 Biotech, vol. 3, issue 1, pp. 45-52.

[20] Butenko, R. G. (1963). Plant Cell Totipotency and Tissue Culture. Culture of Isolated Organs, Tissues and Cells of Plants. Presented at Proceedings of the I All-Union Conference, January Moscow, Russia, pp. 22-26.

[21] Dubenok, N. N., et al. (2012). Growing Eggplant with Drip Irrigation using Tunnel Shelters to Obtain Early Production. Achievements of Science and Technology of the Agro-Industrial Complex, vol. 9. pp. 127-140.

[22] Borodychev, V. V. and Lukyanenko, E. A. (2007). Water Regime and Nutrition of Eggplant with Drip Irrigation. Potatoes and Vegetables, vol. 6, pp. 19-20.

[23] Ano, G., et al. (1991). A New Source of Resistance to Bacterial Wilt of Eggplants Obtained from a Cross: Solanum aethiopicum L× Solanum melongena L., Agronomie vol. 19 issue 7, pp. 555-560.