Optimization Studies of Culture Media for In-Vitro Clonal Micropropagation of New Grape Varieties
Abstract
This article describes the effect of Gautheret, White, Heller and Murashige & Skoog mineral salts during in-vitro clonal micropropagation of new grape varieties. The optimal mineral compositions of the culture medium that support the in-vitro regeneration of isolated grape explants were identified. The grapes that were studied were the Bart and Augustine varieties. Primary grape explants were cultivated for 30 days in a nontransplanted culture. Increased regenerative activity was observed in the Murashige & Skoog and White media. Increased haemogenesis occurred and shoots regenerated. The addition of cytokinin 6-BAP to the medium for obtaining aseptic culture led to an increase in the frequency of shoot-bud production by 5 to 6 times, depending on the type of medium. Combining 6-BAP with the auxin NAA provided an additional increase in the frequency of shoot-bud production, but to a lesser extent. Adding growth regulators to the culture medium also reduced the frequency of explant necrosis.
Keywords: grapes, mineral salts, culture medium, microclonal propagation, in-vitro, cytokinins, auxins
References
[1] Batukaev, A. A. (1998). Improving the Technology of Accelerated Propagation and Sanitation of Grape Planting Material by the In Vitro Method Moscow: Ministry of Agriculture, p. 222.
[2] Bratkova, L. G. and Tsatsenko, N. N. (2015). Clonal Micropropagation of Grapes. Achievements of Science and Technology of the Agro-Industrial Complex, vol. 29, issue 6. pp. 49-52.
[3] Gud, L. A., Kalashnikova, E. A. and Tarakanov, I. G. (2019). Influence of the Spectral Composition of Light on the Morphogenetic Potential of Blackberries and Raspberries In Vitro. Forestry Information, vol.s 2, pp. 97-101.
[4] Dospekhov, B. A. (2011). Methods of Field Experience. Alliance, vol. 6, pp. 351-352.
[5] Kornatsky, S. A., et al. (2018) A Method of Adaptation In Vivo of Strawberry Micro-Plants. RF Patent No. 2642085.
[6] Yermishin, A. P. (2015). Plant Biotechnology and Biosafety: a Manual. Minsk: BSU, p. 359.
[7] : Batukaev, A. A., et al. (2013). A Method of Microcutting In Vitro. Patent No. 2521992 Russian Federation, IPCA01G 17/02.
[8] Medvedeva, N. I., et al. (2008). Features of Microclonal Reproduction of Introduced and Clones of Grapes. Scientific Journal. Kub SAU, vol. 40, issue 6, pp. 137-155.
[9] Kalashnikova, E. A. (2019). Technology for Adapting Vitis Vinifera Microclones to Ex Vitro Conditions. Problems of Agricultural Development in the Region, vol. 3, issue 39, pp. 69-74.
[10] Kornatsky, S. A. (2016). Technological Approaches to the Use of the In Vitro Method for Mass Production of Stone Crop Pants. International Research Journal, vol. 10, issue 52, pp. 150-152.
[11] Kornatsky, S. A. Features of Rooting In Vitro of Everbearing Raspberry Microcutting. Fruit and Berry Growing in Russia, vol. 48, issue 1, 2017, pp. 136-139.
[12] Batukaev, A. A. (2018) Use of Growth Regulators in Grapes Grinding by In Vitro Method. International Multidisciplinary Scientific GeoConference SGEM, vol. 18, issue 6, pp. 783-790.
[13] Batukaev, A. A. (2016) Ion Association in Water Solution of Soil and Vadose Zone of Chestnut Saline Solonetz as a Driver of Terrestrial Carbon Sink. Solid Earth, vol. 7, issue 2, pp. 415-423.
[14] Maghradze, D., et al. (2015). Micropropagation and In Vitro Germplasm Conservation of Georgian Wild Grapevines. Vitis, vol. 54, pp. 257-258.