Containment of Cattle Brucellosis Outbreaks With a Small Dose of Vaccine From Strain 82
Improving the system of anti-brucellosis measures is the ultimate goal of all studies devoted to the problem of brucellosis. Cattle brucellosis outbreaks in the brucellosisfree regions in the Russian Federation indicate a premature cessation of immunization procedures and the need for their continuation. Meanwhile, there is an urgent problem of developing anti-brucellosis immunity in the adult cattle: the initial vaccination of cattle with a full dose of the vaccine from strain B. abortus 82 is fraught with post-vaccination abortions. In order to solve this problem, in the production conditions of one of the farms where the current epizootic situation for brucellosis required alternative approaches for containment of a brucellosis outbreak, a small dose of the vaccine was tested on the cattle without specific immunity. This resulted in immunogenicity, loss of abortogenicity and the presence of provocative properties of the vaccine in small doses. This allowed the detection of antibodies in animals with a hidden infection in the early stages after vaccination. Serological methods (AT, RBT and IDR with O-polysaccharide antigen), which are generally accepted in veterinary practice, were used as diagnostic tests. The reimmunization of the cows with a small dose of the vaccine from strain 82 allowed diagnostic tests to be carried out earlier after vaccination than after the use of a full dose of the vaccine, which made it possible to diagnose brucellosis in a short period of time after vaccination. The provocation of immunological reactions in latently sick animals after the vaccination with small doses, and the isolation of those who responded under the condition of supported immunity made it possible to achieve the recovery of farm animals in a short period of time, which was proved by negative indicators of the immunodiffusion reaction with O-polysaccharide antigen.
Keywords: brucellosis, small doses of the vaccine, abortogenicity, immunity
 Maskaev, N. N., et al. (1988) Immunological Reactions and Immunity in Cattle Vaccinated with AntiBrucellosis Vaccines in Different Doses. In Methods of Diagnosis and Prevention of Animal Brucellosis and Tuberculosis: Research Materials of VNIIBTZH. (pp. 26-31) Omsk: SO VASKHNIL
 Novitsky, A. A. (2001). Omsk Region as a Model for Creating a System of Anti-Brucellosis Measures. Infectious Animal Pathology: Research Materials of SO RASKHN. Omsk: VNIIBTZH.
 Novitsky, A. A., et al. (2019). Areas of Concern of Brucellosis Specific Prevention. Advances in Animal and Veterinary Sciences, vol. 7, pp. 94-99.
 Kosilov, I. A. (1985). The Role of Specific Prevention in the System of Anti-Brucellosis Measures. In Epizootol. and Infection Control Measures against Animal Diseases: Research Materials. Novosibirsk: SO VASKHNIL.
 Salmakov, K. M. (1970). Testing a Vaccine from a Slightly Agglutinogenic Strain of Brucella 82 In Cattle. Research Notes of Kazan Vet. Inst., vol. 107, pp. 83–87.
 Yudina, Е. and Konovalov, S. (2019). Development Issues and Prospects of Milk Processing Enterprises. Presented at The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector” (TFTS 2019): Vol. 393). October 15th, 2019 (Omsk, Russia). Electronic Materials (pp 436-439) Paris: Atlantis Press.
 Albertyan, M. P. (1990). Immunogenicity of Vaccines from Strains 19, 104 M and 82 for Cattle in Various Schemes of Application. Bulletin VIEV, vol. 73. pp. 97-103.
 Grinko, V. K., et al. (1987). Study of the Effectiveness of Immunization of Cattle with a Vaccine from Strain 19 in a Small Dose. Actual problems of veterinary science and practice: Abstr. of Scient Conf. March 26-27th, 1987 (Samarkand, Uzbek SSR). (pp. 87) Samarkand: SO VASKHNIL.
 Dimova, A. S. (2003). Efficiency of Various Anti-Brucellosis Vaccines and Schemes for their Use in Cattle and Goats. (Dissertation of the candidate of veterinary sciences dissertation, Novosibirsk: IEVSDV).
 Kim, V. I. (1989). The Results of Testing the Vaccine from Strain 19 in a Small Dose in the Kyrgyz SSR. In Abstracts of the All-Union Conference. Moscow: Znanie Novosibirsk.
 Minzhasov, K. I. (1986). The Effectiveness of Vaccination of Cattle with a Vaccine from Strain 19 in a Small Dose. In K. I. Minzhasov, (Ed.), Problems of Intensification of Animal Husbandry in Kazakhstan. (pp. 75-76) Alma-Ata: Kazakh republican government scientific and technical society of agriculture.
 Romakhov, V. A., et al. (1989). Epizootological Efficacy of a Vaccine from Strain 19 in a Small Dose against Cattle Brucellosis on Farms in the Samarkand region of the Uzbek SSR. Abstracts of the All-Union Conference. Moscow: Znanie Novosibirsk.
 Rusakov, Y. V., et al. (1989). Immunity against Brucellosis in Cattle Reimmunized with Small Doses of Agglutinogenic Vaccines. Actual Problems of Tuberculosis and Brucellosis in Agricultural Animals: Research Materials of VASKHNIL. Novosibirsk: Sib. Department.
 Khlystunov, A. G., et al. (1988). Immunological Reactivity of Heifers after Revaccination with Small Doses of the Vaccine from Strains 19 and 104 M. Novosibirsk: VASKHNIL, IEVSDV.
 Krasikov, A., et al. (2019). Dynamics of Immunological Parameters of Cattle Infected with Leukemia Virus and its Correction with Betulin-Based Medication. The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019): Vol. 393. October 15th, 2019 (Omsk, Russia). Electronic Materials (pp 25-28) Paris: Atlantis Press. Striełkowski).
 Diaz, R. (1979). Radial Immunodifusion Test with a Brucella Polysaccharide Antigen for Differentiating Infected from Vaccinated. Journal of Clinical Microbiology, vol. 10, pp. 37-41.
 Cherwonogrodzky, J. W. (1988). Brucella abortus 1119-3 O-chain Polysaccharide to Differentiate Sera from B. abortus S-19 - Vaccinated and Fieldstrain Infected Cattle by Agar Gel Immunodiffusion. Journal of Clinical Microbiology., vol. 26, issue 6, pp. 1120 – 1123.