Problems of Managing Potato Plant Growth Under Micropropagation for Primary Seeds


This study shows the possibility of controlling the growth of potato microplants when adding the Murashige-Skoog (MS) nutrient medium chlorocholine chloride (CCC) to the composition at a concentration of 0.05-0.25 mg ⋅ L −1. Varietal specificity of the reaction of potato plants to the preparation was revealed. At optimal concentrations of CCC, plants with a stem length of 7-10 cm in 250 ml flasks were grown from single-node microcuttings for 1.5 months. Microplants were removed from the flasks using 20 cm scissors and unsterilized tweezers. The roots were shortened to 2-3 mm and the basal part of the stems was treated with a chalk-based paste containing 0.04% indolylbutyric acid (IBA). Plants were planted on 36 mm diameter Jiffy 7 peat pellets directly in the laboratory. The trays with the material were transferred to the greenhouse at the end of April at a temperature of 20-25 °C and placed in the conditions of the upper fine irrigation. After 3-4 days, mass root formation began in the plants, and another week later the seedlings were ready for use. By then the height of the plants was 10-15 cm, and the roots were clearly visible on the surface of the peat pellets. Planting these seedlings in a technological greenhouse to obtain minitubers was convenient and inexpensive.

Keywords: potato, microcuttings, microtubers, minitubers, chlorocholine chloride, peat pellets

[1] Donnelly, D. J., Coleman, W. K. and Coleman, S. E. (2003). Potato Microtuber Production and Performance: A Review. American Journal of Potato Research, vol. 80, pp. 103-115.

[2] Morais, T. P., et al. (2018). Application of Tissue Culture Techniques in Potato.Bioscience Journal, vol. 34, issue 4, pp. 952-969.

[3] Salem, J. and Hassanein, A.M. (2017). In Vitro Propagation, Microtuberization and Molecular Characterization of Three Potato Cultivars. BiologiaPlantarum, vol. 61, issue 3, pp. 427-437.

[4] Xhulaj, D. andGixhari,B. (2018). InVitroMicropropagation of Potato (Solanum TuberosumL). Cultivars.Agriculture &Forestry, vol. 64, issue 4, pp.105-112.

[5] Hussey, G. and Stacey,N. J. (1981).In Vitro Propagation of Potato (Solanum tuberosum L.).Annals of Botany, vol. 48, issue 6,pp.787–796.

[6] Bamberg, J. B., et al. (2016). In Vitro Technology at the US Potato Genebank. In Vitro Cellular & Developmental Biology-Plant, vol. 52, issue 3, pp. 213–225.

[7] Westcott, R.J., et al. (1977). Tissue Culture Methods and Germplasm Storage in Potato. Acta Horticulturae, vol. 78, pp. 45-50.

[8] Murashige, T. and Skoog, F. (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Cultures. Physiologia Plantarum, vol. 15, pp. 473-497.

[9] Khuri, S. andMoorby, J. (1996).Nodal Segments or Microtubersas Explants for in Vitro Microtuber Production of Potato.Plant Cell, Tissue and Organ Culture, vol. 45, pp. 215-222.

[10] Shah, A. H.,et al. (2003). Cost Effective Micropropagation Technology for Potatoes. Pakistan Journal of Biological Sciences, vol. 6, issue 4, pp. 336-340.

[11] Janet, E. A., et al. (2004). Effect of Leaves on Microtubers Produced from Potato Single-Node Cuttings In Vitro. American Journal of Potato Research, vol. 81, pp. 1-5.

[12] Borna, R., Hoque, M. and Sarker, R. (2019). In Vitro Microtuber Induction and Regeneration of Plantlets from Microtuber Discs of Cultivated Potato (Solanum tuberosum L.). Plant Tissue Culture and Biotechnology, vol. 29, issue 1, pp. 63-72.

[13] Suttle, J. C., Huckle, L. L. and Lula, E. C. (2011). The Effects of Dormancy Status on the Endogenous Contents and Biological Activities of Jasmonic Acid, N-( jasmonoyl)-Isoleucine and Tuberonic Acid in Potato Tubers. American Journal of Potato Research, vol. 88, pp. 283–293.

[14] Muñoz, M., et al. (2019). Slow Growth in Vitro Culture for Conservation of ChilotanumpotatoGermplasm.Chilean Journal of Agricultural Research, vol. 79, issue 1, pp. 29- 35.

[15] Kornatskiy, S.A. (2017). A Method for Producing Potato Minitubers. Patent of the Russian Federation No. 2621571.

[16] Kornatskiy, S.A., Popkova, A.A. and Semenov, A.G. (2018). Experience in the Use of Potato Seedlings for Growing Minitubers in Greenhouse.Theoretical & Applied Problems of Agro-Industry, vol. 2, issue 35, pp.7-11.