Evaluation of Strawberry Seedling Growth in Various Planting Media Amended with Biofertilizer

Abstract

The quality of strawberry seedlings is impacted by the composition of the growing medium. Due to its good drainage and high water-holding capacity, coco-peat is now widely used as a major component of strawberry substrate; however, coco-peat has a low nutrient content. By combining coco-peat with manure and biofertilizer, more nutrients can be provided. This study investigated the effect of the growing medium composition with biofertilizer inoculation on the growth and quality of strawberry seedlings in a mountainous area using a pot experiment. With six treatments and five replications, the experiment was set up in randomized block design. The treatments involved substrate composition, which included cocopeat and chicken manure in ratios of 2:1, 2:3 and 3:2, and biofertilizer inoculation (with and without). Nitrogen-fixing bacteria and phosphate-solubilizing microbes were included in the biofertilizer. The results showed that substrate composition affected plant height, shoot and root dry weight, chlorophyll content, and nitrogen and phosphorus uptake by seedling shoots, but not the surface area of leaves on six-week-old seedlings. The addition of biofertilizer to substrates made up of cocopeat and chicken manure in ratios of 2:3 and 3:2 increased plant growth and nitrogen and phosphorus uptake.


Keywords: Chicken namnure, Cocopeat, N2 -fixer bacteria, P-solublizer Microbes.

References
[1] Asiah A, Razi IM, Khanif YM, Marziah M, Shaharuddin M. Physical and chemical properties of coconut coir dust and oil palm empty fruit bunch and the growth of hybrid heat tolerant cauliflower plant. Pertanika Journal of Tropical Agricultural Science. 2004;27(2):121-133.

[2] Hindersah R, Rahmadina I, Fitriatin BN, Setiawati MR, Indrawibawa D. Microbescoated urea for reducing urea dose of strawberry early growth in soilless media. Jordan Journal of Biological Science. 2021;14(3):593–599.

[3] Asghari R. Effect of growth medium and nutrient solution on phytochemical and nutritional characteristics of strawberry (Fragaria x Ananassa duch.). Journal of Agricultural Science. 2012;6(8):52-59.

[4] Shanmugasundaram R, Jeyalakshmi T, Mohan SS, Saravanan M, Goparaju A, Murthy PB. Coco peat – An alternative artificial soil ingredient for the earthworm toxicity testing. Journal of Toxicology and. Environmengal Health Science. 2014;6:5-12.

[5] Treder W, Tryngiel-Gać A, Klamkowski K. Development of greenhouse soilless system for production of strawberry potted plantlets. Horticulural Science. 2015;42(1):29–36.

[6] Schäfer M, Brütting C, Baldwin IT, Kallenbach M. High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC–HESI– MS/MS. Plant Methods. 2016;12(1):1-18.

[7] Kumra R, Saravanan RS, Bakshi P, Kumar A, Singh M, Kumar V. Influence of plant growth regulators on strawberry: A review. International Journal of Chemical Studies. 2018;6(1):1236-1239.

[8] Rishu S, Kumar SS, Kumar SS, Sanjay S, Sonam. Cytokinin - A potential plant growth regulator for strawberry (Fragaria x Ananassa duch.) production. Research Journal of Chemistry and Environment. 2019;23(5):107-113.

[9] Mishra AN, Tripathi VK. Influence of different levels of Azotobacter, PSB alone and in combination on vegetative growth, flowering, yield and quality of strawberry cv. Chandler. International Journal of Applied Agricultural Research. 2011;6(3):203-210.

[10] Reddy GC, Goyal RK. Growth, yield and quality of strawberry as affected by fertilizer N rate and biofertilizers inoculation under greenhouse conditions. Journal of Plant Nutrition. 2020;44(1):46-58.

[11] Hindersah R, Suryatmana P, Fitriatin BN, Setiawati MR. Effect of liquid biofertilizer on soil nitrogen and phosphorous, and yield of choy sum (Brassica rapa L.) growing in pot culture. International Journal of Research in Engineering and Science. 2017;5(2):61-66.

[12] Hindersah R, Kalay AM, Kesaulya H, Suherman C. The nutmeg seedlings growth under pot culture with biofertilizers inoculation. Open Agriculture. 2021;6(1):1-10.

[13] Ben-David A, Davidson CE. Estimation method for serial dilution experiments. Journal of Experimental Methods. 2014;107:214-221.

[14] Easlon HM, Bloom AJ. Easy leaf area: automated digital image analysis for rapid and accurate measurement of leaf area. Applled Plant Science. 2014;2(7):1400033.

[15] Braniša J, Jenisová Z, Porubská M, Jomová K, Valko M. Spectrophotometric determination of chlorophylls and carotenoids. an effect of sonication and sample processing. Journal of Microbiology, Biotechnology and Food Science. 2014;3(2):61- 64.

[16] Association of Official Analytical Chemists (AOAC). Official methods of analysis of AOAC international. 19th ed. . Gaithersburg: AOAC; 2012.

[17] Gomez KA, Gomez AA. Prosedur statistik untuk penelitian pertanian. Jakarta: Universitas Indonesia Press; 2007.

[18] Hanif Z. Budidaya stroberi (Fragaria x ananassa). Balai penelitian tanaman jeruk dan buah suptropika (Balitjestro). Balitjestro; 2015. Available from: http://balitjestro.litbang.pertanian.go.id/budidaya-stroberi-fragaria-x-ananassa/

[19] Karajeh MR, Al-Rawashdeh ZB, Al-Ramamneh EAM. Occurrence and control of strawberry powdery mildew in Al-Shoubak/Jordan. Jordan Journal of Agricultural Science. 2012;8(3):380-390.

[20] Taiz L, Zeiger E, Møller M, Murphy A. Plant physiology. 6th ed. Sunderland: Sinauer Associates; 2014.

[21] García-Caparrós P, Lao MT, Preciado-Rangel P, Sanchez E. Phosphorus and carbohydrate metabolism in green bean plants subjected to increasing phosphorus concentration in the nutrient solution. Agronomy. 2021;11(2):245.