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Amouzeshi, A., Zahra Moossavi, S., Yoosef Javad Moosavi, S., Zardast, M., Malekaneh, M., Esmaili, S., Taneh, A., Lotfi, N., Moossavi, M., & Hoshyar, R. (2019). Aqueous Cannabis Extract Prevents Ethylene Glycol-induced Renal Calcium Crystallization. Research in Molecular Medicine (RMM), 7(1). https://doi.org/10.18502/rmm.v7i1.5255

https://doi.org/10.18502/rmm.v7i1.5255

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authors

  • Ahmad Amouzeshi

    Ahmad Amouzeshi

    Department of Cardiovascular Surgery, Surgery and Trauma Research Group, Birjand University of Medical Sciences, Birjand, Iran
  • Seyedeh Zahra Moossavi

    Seyedeh Zahra Moossavi

    Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
  • Seyed Yoosef Javad Moosavi

    Seyed Yoosef Javad Moosavi

    Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
  • Mahmoud Zardast

    Mahmoud Zardast

    Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
  • Mohammad Malekaneh

    Mohammad Malekaneh

    Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
  • Sajad Esmaili

    Sajad Esmaili

    Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
  • Abdolghader Taneh

    Abdolghader Taneh

    Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
  • Nasim Lotfi

    Nasim Lotfi

    Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran
  • Maryam Moossavi

    Maryam Moossavi

    Student Research Committee, Birjand University of Medical Sciences, Birjand, Ira
  • Reyhane Hoshyar

    Reyhane Hoshyar

    Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran; Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA

Published Date

  • Sep 30, 2019

Aqueous Cannabis Extract Prevents Ethylene Glycol-induced Renal Calcium Crystallization

Research in Molecular Medicine (RMM) / Research in Molecular Medicine (RMM): Volume 7, Issue No. 1 /

Abstract

Background: Some plant extracts can prevent kidney stone formation in a dosedependent manner. In our study, we aimed to investigate the protective role of Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycolinduced rats. Materials and Methods: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of Cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated. Results: Results showed that 100 mg/kg and 200 mg/kg doses of Cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05). Conclusion: Histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy.