TY - JOUR AU - Ahmad Amouzeshi AU - Seyedeh Zahra Moossavi AU - Seyed Yoosef Javad Moosavi AU - Mahmoud Zardast AU - Mohammad Malekaneh AU - Sajad Esmaili AU - Abdolghader Taneh AU - Nasim Lotfi AU - Maryam Moossavi AU - Reyhane Hoshyar PY - 2019/09/30 Y2 - 2024/03/29 TI - Aqueous Cannabis Extract Prevents Ethylene Glycol-induced Renal Calcium Crystallization JF - Research in Molecular Medicine (RMM) JA - RMM VL - 7 IS - 1 SE - Articles DO - 10.18502/rmm.v7i1.5255 UR - https://knepublishing.com/index.php/rmm/article/view/5255 AB - Background: Some plant extracts can prevent kidney stone formation in a dosedependent manner. In our study, we aimed to investigate the protective role of Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycolinduced rats. Materials and Methods: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of Cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated. Results: Results showed that 100 mg/kg and 200 mg/kg doses of Cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<0.05). Conclusion: Histopathological slides showed reduced calcifications with extract treatment at 200 mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy. ER -