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Febriani, A. L., Anggraini, R., Naila Mazieda, M., Saptasari, M., Listyorini, D., & Fatma Wardani, F. (2017). Isolation of Flower Development Regulator Gene SEPALLATA 1 in Phalaenopsis amabilis (L.) Blume. KnE Life Sciences, 3(4), 54-60. https://doi.org/10.18502/kls.v3i4.687

https://doi.org/10.18502/kls.v3i4.687

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authors

  • Ayu Linda Febriani
    No author data available.
  • Riska Anggraini
    No author data available.
  • Muthia Naila Mazieda
    No author data available.
  • Murni Saptasari
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  • Dwi Listyorini
    No author data available.
  • Fitri Fatma Wardani
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Published Date

  • Jul 11, 2017

Isolation of Flower Development Regulator Gene SEPALLATA 1 in Phalaenopsis amabilis (L.) Blume

KnE Life Sciences / The 4th International Conference on Biological Science (2015) / Pages 54-60

Abstract

Phalaenopsis amabilis (L.) Blume is an indigenous orchid species in Indonesia. This orchid has a white large flower. The large flower is caused by the existence of gene that has an important role in flower development. One of the genes is SEPALLATA 1. This gene is a member of superfamily MADS-Box gene. SEPALLATA 1 gene is a marker of primordial flower organ. This study aimed to isolate SEPALLATA1 gene from Phalaenopsis amabilis (L.) Blume by PCR using forward primer 5’-GCT-GGA-GCG-GAT-CGA-GAA-CA-3’and reverse primer 5’-TCA-TGC-AAG-CCA-ACC-AGG-TG-3’. This study successfully amplified 691 bp lengths of SEPPALATA1 fragment, lacking 20 bp upstream which consist its start codon.

 

Keywords: Flower development regulation, Phalaenopsis amabilis (L.) Blume, SEPALLATA 1 gene.

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