The Effect of Osmopriming on Germination and Seedling Growth of Glycine Max L. Under Salinity Stress


Soybean (Glycine max L.) is a staple food crop in Indonesia. Soybean plants have decreased in quality and production due to salinity factors. Planting media with high salinity conditions have limited potential for plant cultivation, but each plant has different resistance and adaptability. Osmotic priming of seeds or osmopriming could increase plant resistance to salinity. This experiment was conducted at Universitas Muria Kudus Field. The objective of this study was to determine the germination and seedling growth of Glycine max L. as treated by NaCl osmopriming. A randomized complete design was used with two factors. The first factor was osmopriming concentration which consisted of the control, 50 mM (K1), 100 mM (K2), and 150 mM (K3). The seeds of the control did not receive osmopriming NaCl treatment. The second factor was osmopriming duration which consisted of 24 hours (T1) and 48 (T2). The results showed that the treatment of osmopriming with a concentration of 50 mM NaCl for a duration of 24 hours had a positive impact on seed germination under salinity conditions of 150 mM NaCl. However, this did not significantly affect the seedling growth in salinity conditions of 150 mM NaCl.

Keywords: osmopriming, salinity, soybean

[1] Kristiono A, Purwaningrahayu RD, Taufiq A. Respons tanaman kedelai, kacang tanah, dan kacang hijau terhadap cekaman salinitas. Buletin Palawija. 2018;26:45-60.

[2] Sobir N, Helmi S. Respon morfologi dan fisiologi genotype Terung (Solanum melongena L.) terhadap cekaman salinitas. J. Hort. Indonesia. 2018;2:131-138.

[3] KaldyJE,ShaferDJ,AilstockMS,MagounAD.Effectsoftemperature,salinityandseedageoninduction of Zostera japonica germination in North America, USA. Aquatic Botany. 2015;126:73-79.

[4] Tabassum T, Farooq M, Ahmad R, Zohaib A, Wahid A, Shahid M. Terminal drought and seed priming improves drought tolerance in wheat. Physiology and Molecular Biology of Plants. 2018;24:845-856.

[5] Sulaiman S, Suwignyo RA, Hasmeda M, Wijaya A. Priming benih padi (Oryza sativa L.) dengan Zn untuk meningkatkan vigor bibit pada cekaman terendam. Jurnal Agronomi Indonesia. 2016;44:8-15.

[6] Langeroodi ARS, Noora R. Seed priming improves the germination and field performance of soybean under drought stress. The Journal of Animal and Plant Sciences. 2017;5:1611-1621.

[7] Baskin CC, Baskin JM. Seeds: ecology, biogeography, and evolution of dormancy and germination. London: Elsevier; 1998.

[8] Souza MO, Pelacani CR, Willems LA, Castro RD, Hilhorst HW, Ligterink W. Effect of osmopriming on germination and initial growth of Physalis angulata L. under salt stress and on expression of associated genes. Anais da Academia Brasileira de Ciências. 2016;88:503-516.

[9] Jasmi J. Pengaruh konsentrasi NacL dan varietas terhadap viabilitas, vigor dan pertumbuhan vegetatif benih kacang hijau (Vigna radiata L.). Jurnal Agrotek Lestari. 2018;2:11-22.

[10] Abdallah EH, Musa Y, Mustafa M, Sjahril R, Riadi M. Comparison between hydro-and osmo-priming to determine period needed for priming indicator and its effect on germination percentage of aerobic rice cultivars (Oryza sativa L.) AGRIVITA, Journal of Agricultural Science. 2016;38:222-30.

[11] Brooks S, Athinuwat D, Chiangmai PN. Enhancing germination and seedling vigor of upland rice seed under salinity and water stresses by osmopriming. Science & Technology Asia. 2020;25:63-74.

[12] Ismail AI, El-Araby MM, Hegazi AZ, Moustafa SM. Optimization of priming benefits in tomato (Lycopersicon esculentum M.) and changes in some osmolytes during the hydration phase. Asian Journal of Plant Sciences. 2005;4:691-701.

[13] Moshtaghi-Khavaran A, Khomari S, Zare N. Soybean seed germination and seedling growth in response to deterioration and priming: effect of seed size. Plant Breeding and Seed Science. 2014;70:55-67.