Transcription Pattern of Catalase Gene from Gynostemma pentaphyllum (Thunb.) Makino during Various Abiotic Stresses
Abstract
Catalase (CAT) is a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species. A CAT cDNA was previously isolated and characterized from 3-month-old hydroponically cultured Gynostemma pentaphyllum (Thunb.) Makino plants. The ORF is 1.479 bp with a deduced amino acid sequence of 492 residues. CAT from G. pentaphyllum has a molecular mass of 56.97 kDa with an isoelectric point (pI) of 6.95. The temporal expression analysis of leaf samples demonstrated that GpCAT expression could be up-regulated by various environmental stresses such as jasmonic acid electro, oxidative, salt, heavy metal, chilling and heat stress in a certain time period. A three-dimensional structural model of G. pentaphyllum based on its GpCAT cDNA sequence. The temporal expression pattern suggests that the GpCAT could play a role in the molecular defense response of G. pentaphyllum to abiotic stresses.
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