TY - JOUR AU - Johan Sukweenadhi AU - Yu-Jin Kim AU - Deok-Chun Yang PY - 2017/09/11 Y2 - 2024/03/29 TI - Transcription Pattern of Catalase Gene from Gynostemma pentaphyllum (Thunb.) Makino during Various Abiotic Stresses JF - KnE Life Sciences JA - KLS VL - 3 IS - 5 SE - Articles DO - 10.18502/kls.v3i5.982 UR - https://knepublishing.com/index.php/KnE-Life/article/view/982 AB - Catalase (CAT) is a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species. A CAT cDNA was previously isolated and characterized from 3-month-old hydroponically cultured Gynostemma pentaphyllum (Thunb.) Makino plants. The ORF is 1.479 bp with a deduced amino acid sequence of 492 residues. CAT from G. pentaphyllum has a molecular mass of 56.97 kDa with an isoelectric point (pI) of 6.95. The temporal expression analysis of leaf samples demonstrated that GpCAT expression could be up-regulated by various environmental stresses such as jasmonic acid electro, oxidative, salt, heavy metal, chilling and heat stress in a certain time period. A three-dimensional structural model of G. pentaphyllum based on its GpCAT cDNA sequence. The temporal expression pattern suggests that the GpCAT could play a role in the molecular defense response of G. pentaphyllum to abiotic stresses. ER -