Comprehensive Assessment of Promising Potato Hybrids of Breeding VSC RAS


Using the traditional and marker-assisted selection methods, a comprehensive assessment of promising hybrids from the collection of the All-Russian Scientific Center was carried out. The assessment was conducted in 2018–2019 in the Republic of North Ossetia-Alania. As a result of molecular genetic analysis, hybrids were found with complex resistance to potato nematode, virus Y and X viruses - 2 / V, 5 / V, 6 / V, 17 / V, 40 / V, 43 / V, 46 / V, 54 / V, 124 / V, 9 / VI, 22a / VI, 35 / VI, 130 / VI, 71 / VII and 118 / VIII. Use of these selected forms allows optimal protection of potatoes, limitation of the spread of pathogens and prevention of the emergence of more aggressive pathotypes (races and strains). The hybrids with resistance to potato virus Y (with the presence of R-gene markers - 1/I, 3/I, 10/I, 13/I, 11/II, 15/III, 2/V, 5/V, 6/V, 7/V, 10/V/1140, 17/V, 40/V, 43/V, 46/V, 54/V, 124/V, 9/VI, 22a/VI, 35/VI, 100/VI, 130/VI, 71/VII) are of interest for practical breeding, as well as the hybrids with resistance to Phytophthora infestans such as 15/III, 119/IX and the hybrids 15 / III, 35 / VI, 130 / VI and 71 / VII, which have high marketable yield and weight of tuber.

Keywords: potato, interspecific hybrids, marker-assisted breeding, resistance genes

[1] Barone, A. (2004). Molecular Marker-Assisted Selection for Potato Breeding. American Journal of Potato Research, vol. 81, pp. 111-117.

[2] Gebhardt, C., et al. (2006). Marker-Assisted Combination of Major Genes for Pathogen Resistance in Potato. Theoretical and Applied Genetics, vol. 112, pp. 1458-1464.

[3] Simakov, E. A., Sklyarova, N. P. and Yashina, I. M. (2006). Methodical Instructions on the Technology of the Potato Breeding Process. Moscow: Editorial Office of the Journal, p. 72.

[4] Sagai-Maroof, M. A., et al. (1984). Ribosomal DNA Spacer-Length Polymorphism in Barley: Mendelian Inheritance, Chromosomal Location, and Population Dynamics. Proceedings of the National Academy of Sciences, vol. 81, pp. 8014-8018.

[5] Song, Y. S. and Schwarzfischer, A. (2008). Development of STS Markers for Selection of Extreme Resistance (Rysto) to PVY and Maternal Pedigree Analysis of Extremely Resistant Cultivars. American Journal of Potato Research., vol. 85, pp. 159-170.

[6] Kasai, K., et al. (2000). Development of SCAR Markers to the PVY Resistance Gene Ryadg Based on a Common Feature of Plant Disease Resistance Genes. Genome, vol. 43, pp. 1-8.

[7] Mori, K., et al. (2011). Development of a Multiplex PCR Method for Simultaneous Detection of Diagnostic DNA Markers of Five Disease and Pest Resistance Genes in Potato. Euphytica, vol. 180, pp. 347-355.

[8] Galek, R., et al. (2011). Application of DNA Markers Linked to the Potato H1 Gene Conferring Resistance to Pathotype Ro1 of Globodera Rostochiensis. Journal of Applied Genetics, vol. 52, pp. 407-411.

[9] Schultz, L., et al. (2012). Evaluation and Implementation of a Potential Diagnostic Molecular Marker for H1-Conferred Potato Cyst Nematode Resistance in Potato (Solanum tuberosum L). Plant Breed, vol. 131, pp. 315-321.

[10] Asano, K. and Tamiya, S. (2013). Development of Rapid Estimation Method for Allele Number of H1 and Selection of Multiplex Lines in Potato. Ikushugaku kenkyu (Breed. Res.), vol. 15, issue 1, p. 53.

[11] Tomczynska, I., et al. (2014). Hypersensitive Response to Potato virus Y in Potato Cultivar Sarpo Mira is Conferred by the Ny-Smira Gene Located on the Long Arm of Chromosome IX. Molecular Breeding, vol. 34, issue 2, pp. 471-480.