Collagen Isolation from Arctic Marine Organisms and Their Industrial Processing Wastes


The results of the isolation of collagen hydrolysates from tissues of two Arctic marine organisms are presented. Extraction and use of marine organism collagen is a part of complex waste less processing of sea catches. Possible ways of preparing water-soluble collagen hydrolysates from different sources were studied. It is established that for preparing soluble collagen hydrolysate from skin of cod Gadus morhua acid hydrolysis in 0.3% acetic acid is suitable. Purification of solution by means of ultrafiltration gives a pure collagen hydrolysate with mass fraction of the main substance about 98%. Collagen of muscular skin bag of holothuria Molpadia borealis is almost insoluble in acid and alkali solutions. The major mass of collagen remains in an insoluble residue. The analysis of infrared spectrum transmission and chemical analysis of the general carbohydrates and collagen in different fractions showed that as a result of acid and alkaline processing of raw materials the glycosaminoglycans (GAG) and some quantity of collagen are extracted, their main quantity was determined in alkaline extracts. For extraction of soluble collagen from insoluble residue it is required enzymatic by pepsin in acidic medium. Properties of holothurian collagen and fish collagen are different. The preparation of water-soluble collagen derivatives requires using enzymatic hydrolysis.

[1] Ed. P. Fratzl. (2008). Collagen. Structure and Mechanics. New York: Springer Science+Business Media, LLC.

[2] Ed. M. A. Karsdal. (2016). Biochemistry of Collagens, Laminins and Elastin. Structure, Function and Biomarkers. Amsterdam, Boston et al.: Academic Press.

[3] Brinckmann, J. (2005). Collagens at a glance. In Collagen: Primer in Structure, Processing and Assembly. Series: Topics in Current Chemistry, vol. 247, pp. 1–6.

[4] Moreira-Silva, J., Diogo, G. S., Marques, A. L. P., et al. (2016). Marine collagen isolation and processing envisaging biomedical applications. Biomaterials from nature for advanced devices and therapies, pp. 16–36.

[5] Schmidt, M. M., Dornelles, R. C. P., Mello, R. O., et al. (2016). Collagen extraction process. International Food Research Journal, vol. 23(3), pp. 913–922.

[6] Konstantinova, L.L., Dvinin, Yu. F., Lebskaya, T.K., et al. (1997). Technochemical properties of commercial fish of the North Atlantic and the adjacent Seas of the Arctic Ocean. Murmansk: PINRO Publ. 183 p.

[7] Semyonycheva, L. L., Astanina, M. V., Kuznetsiva Yu. L., et al. (2015). Method for production of acetic dispersion of high molecular fish collagen: Patent RU 2567171: MПK C08H 1/06, A23J 1/04; № 2014140300/13; Appl. 06.10.2014; Publ. 10.11.2015. (in Russian)

[8] Marine proteins and peptides: biological activities and applications / Ed. by Se-Kwon Kim. (2013). John Wiley & Sons, Ltd.

[9] Hongdong, S., Bo, L. (2017). Beneficial effects of collagen hydrolysate: A review on recent developments. Biomedical Journal of Scientific & Technical Research, vol. 1(2), pp. 1–4.

[10] Mukhortova, A. M., Uzbekova, O. R., Lyzhov, I. I., et al. (2018). Comparative technical and chemical properties and promising ways of processing for holothurians Molpadia arctica, Molpadia borealis and Cucumaria frondosa from the Barents and the Kara seas. Rybnoe Chozyaystvo, No. 1, pp. 36-39. (in Russian)

[11] Trotter, J. A., Lyons-Levy, G., Thurmond, F. A., Koob, T. J. (1995). Covalent composition of collagen fibrils from the dermis of the sea cucumber, Cucumaria frondosa, a tissue with mutable mechanical properties. Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology, vol. 112 (3–4), pp. 463–478.

[12] Cui, F., Xue, C., Li, Z., et al. (2007). Characterization and subunit composition of collagen from the body wall of sea cucumber Stichopus japonicas. Food Chemistry, vol. 100(3), pp. 1120–1125.

[13] Zhong, M., Chen, T., Hu, C., Ren, C. (2015). Isolation and characterization of collagen from the body wall of sea cucumber Stichopus monotuberculatus. Food Science, vol. 80(4), pp. C671–C679.

[14] Zhu, B.-w., Dong, X.-p., Zhou, D,-y., et al. (2012). Physicochemical properties and radical scavenging capacities of pepsin-solubilized collagen from sea cucumber Stichopus japonicas. Food Hydrocolloids, vol. 28(1), pp. 182–188.

[15] Liu, Z., Oliveira, A. C. V., Su, Y.-C. (2010). Purification and characterization of pepsin-solubilized collagen from skin and connective tissue of giant red sea cucumber (Parastichopus californicus). Journal of Agricultural and Food Chemistry, vol. 58(2), pp. 1270–1274.

[16] Adibzadeh, N., Aminzadeh, S., Jamili, S., et al. (2014). Purification and characterization of pepsinsolubilized collagen from skin of sea cucumber Holothuria parva. Applied Biochemistry and Biotechnology, vol. 173(1), pp. 143–154.

[17] Abedin, M. Z., Karim, A. A., Latiff A. A., et al. (2014). Physicochemical and biochemical properties of pepsin-solubilized collagen isolated from the integument of sea cucumber (Stichopus vastus). Journal of Food Processing and Preservation, vol. 38(4), pp. 2027–2036.

[18] Lisitsyn, A. B., Ivankin, A. N., Neklyudov, A. D. (2002). Practical biotechnology techniques. Moscow: VNIIMP Publ. 402 p. (in Russian)

[19] Mukhin, V. A., Smirnova, E. B., Novikov, V. Yu. (2007). Peculiarities of digestive function of proteinases in invertebrates – inhabitants of cold seas. Journal of Evolutionary Biochemistry and Physiology, vol. 43(5), pp. 476–482.

[20] Moore, S., Stein, W. H. (1963). Chromatographic determination of amino acids by the use of automatic recording equipment. In Methods Enzymology. Vol. 6 / Eds. S. P. Colowick, N. O. Kaplan. San Diego, New York et al.: Academic Press. pp. 819–831.

[21] GOST 33692-2015. (2016). Animal protein of connective tissue. General specifications. Moscow: Standartinform. 27 p. (in Russian)

[22] Jo, J.-H., Do, J.-R., Kim, Y.-M., et al. (2005). Optimization of shark (Squatina oculata) cartilage hydrolysis for the preparation of chondroitin sulfate. Food Science and Biotechnology, vol. 14 (5), pp. 651–655.

[23] GOST 7636-85. (2010). Fish, marine mammals, invertebrates and products of their processing. Methods for analysis. Moscow: Standartinform. (in Russian)

[24] Siddiqui, Y. D., Arief, E. M., Yusoff, A., et al. (2013). Extraction, purification and physical characterization of collagen from body wall of sea cucumber Bohadschia bivitatta. Health and the Environment Journal, vol 4(2), pp. 53–65.

[25] Saito, M., Kunisaki, N., Urano, N., Kimura, S. (2002). Collagen as the major edible component of sea cucumber (Stichopus japonicus). Journal of Food Science, vol. 67(4), pp. 1319–1322.