Phytochemical Profile and Sensory Evaluation of Natural Vinegar from Mixed Fruits and Flowers of Melastoma malabathricum L. with Variations of Starter Concentration and Fermentation Time


Melastoma malabathricum L. is a wild plant in Aceh that can be used as a traditional medicine. This research aimes to determine the phytochemical profile and sensory evaluation of the fruit and flower vinegar of Melastoma malabathricum L. This research used a completely randomized design (CRD) consisting of two main groups, Saccharomyces cerevisae 5 days and 10 days with six treatments. Both the groups were added with Acetobacter aceti concentrations of 5%, 10%, and 15% which were fermented for 28 days. This study conducted phytochemical screening, total phenol and flavonoid test, and sensory evaluation. The results showed that the phytochemical test of the vinegar contained flavonoids, saponins, tannins, polyphenols, and terpenoids. The results of the sensory evaluation or organoleptic test showed that there was no significant difference in the color, acceptability, and Aroma of vinegar in all treatments but there was a significant difference in the taste of vinegar (p<0.05). The preferred vinegar was in the P5 treatment with a neutral taste. This natural vinegar product can be used as a beneficial beverage for public health.

Keywords: phytochemical, sensory evaluation, vinegar, Melastoma malabathricum L, mixed fruits and flowers, starter concentration, fermentation time

[1] Gloria Y, Delfina D, Bachtiar Y. “Effectivity test antibacterial senggani leaf (Melastoma candidum) on bactery Streptococcus mutans.,” Jurnal Biosains. vol. 5, no. 1, p. 2019.

[2] Isnaini I, Yasmina A, Nur’amin HW. Antioxidant and cytotoxicity activities of karamunting (Melastoma malabathricum L.) fruit ethanolic extract and quercetin. Asian Pac J Cancer Prev. 2019 Feb;20(2):639–43.

[3] Aslam M, Ahmad MS, Ahmad MA. An up-to-date review on phytochemical constituents and pharmacological activities of Melastoma malabathricum. Int J Pharm Pharm Sci. 2017;8(5):76–91.

[4] K. Balamurugan, A. Nishanthini, and V.R. Mohan, “Antidiabetic and antihyperlipidaemic activity of ethanol extract of Melastoma malabathricum Linn. leaf in alloxan induced diabetic rats.,” Asian Pacific Journal of Tropical Biomedicine. vol. 4, no. Suppl 1, pp. S442–S448, 2014.

[5] Kamisan FH, Yahya F, Ismail NA, Din SS, Mamat SS, Zabidi Z, et al. Hepatoprotective activity of methanol extract of Melastoma malabathricum leaf in rats. J Acupunct Meridian Stud. 2013 Feb;6(1):52–5.

[6] Ismail Suhaimy NW, Noor Azmi AK, Mohtarrudin N, et al. “Semipurified ethyl acetate partition of methanolic extract of Melastoma malabathricum leaves exerts gastroprotective activity partly via its antioxidant-antisecretory-anti-inflammatory action and synergistic action of several flavonoid-based compounds.,” Oxidative Medicine and Cellular Longevity. vol. 2017, p. 2017.

[7] Leasa H, Matdoan MN. “Pengaruh lama fermentasi terhadap total asam cuka Aren (Arenga pinnata Merr.).,” Biopendix: Jurnal Biologi, Pendidikan dan Terapan. vol. 1, no. 2, pp. 140–145, 2015.

[8] Septiani NK, Parwata IM, Putra AA. “Penentuan kadar total fenol, kadar total flavonoid dan skrining fitokimia ekstrak etanol daun Gaharu (Gyrinops versteegii).,” Jurnal Matematika, Sains, dan Pembelajarannya. vol. 12, no. 1, pp. 78–89, 2018.

[9] Badan Standardisasi Nasional. Sni 2729:2013. Badan Standarisasi Nasional Indonesia; 2013. pp. 1–15.

[10] Capule AB, Barcelon EG. “Influence of colour on the sensory perception of ready-toinfluence of colour on the sensory perception of ready-to-drink soy milk.,” Asian journal of Science and Technology. vol. 5, no. 12, pp. 879–882, 2014.

[11] Wanjiru Maina J, Juliana Wanjiru Maina Murang C. “Analysis of the factors that determine food acceptability.,” ~ 253 ~ The Pharma. Innov J. 2018;7(5):253–7.

[12] Briand L, Salles C. “Taste perception and integration.,” Flavor: From Food to Behaviors, Wellbeing and Health. no. February 2017, pp. 101–119, 2016.

[13] Boesveldt S, de Graaf K. The differential role of smell and taste for eating behavior. Perception. 2017;46(3-4):307–19.

[14] Anwar C. Kajian penggunaan jenis ikan dan tepung terigu pada kualitas kimia, fisik, dan organoleptik Kamaboko. JFMR-Journal of Fisheries and Marine Research. 2019;3(3):288–300.

[15] Kotthoff M. Odor and nutrition -Part 3: food odorants and their analysis. Ernahr- Umsch. 2015;62(5):28–34.