Download fulltext HTML
Sukmawati, D., Firhandini, A., Khumaiya, U., Ayu Komsiatun, D., Gunadi, S. A., Supiyani, A., Susilowati, D. N., Al Husna, S. N., El Enshasy, H., Dailin, D. J., & Sriherwanto, C. (2024). Antagonistic Activity of Cellulase-Producing Yeasts Isolated from Cocoa Fermentation Against Pathogenic Molds Collected from Damaged Cocoa Fruits. KnE Life Sciences, 8(1), 85–96. https://doi.org/10.18502/kls.v8i1.15401

https://doi.org/10.18502/kls.v8i1.15401

statistics

  • 71 Abstract Views
  • 31 PDF Views
  • 0 HTML Views

authors

  • Dalia Sukmawati

    Dalia Sukmawati

    Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
    https://orcid.org/0000-0001-9641-9321
  • Alika Firhandini

    Alika Firhandini

    Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
  • Umi Khumaiya

    Umi Khumaiya

    Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
  • Dwi Ayu Komsiatun

    Dwi Ayu Komsiatun

    Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
  • Syifa Aulia Gunadi

    Syifa Aulia Gunadi

    Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
  • Atin Supiyani

    Atin Supiyani

    Biology Department, Laboratory of Microbiology, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia
  • Dwi Ningsih Susilowati

    Dwi Ningsih Susilowati

    Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Indonesia Jl. Tentara Pelajar No. 3A, Bogor 16111, Jawa Barat, Indonesia
  • Shabrina Nida Al Husna

    Shabrina Nida Al Husna

    Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung, Jawa Barat, Indonesia
  • Hesham El Enshasy

    Hesham El Enshasy

    Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
  • Daniel Joe Dailin

    Daniel Joe Dailin

    Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
  • Catur Sriherwanto

    Catur Sriherwanto

    Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Science and Technology Park, Banten, Indonesia

Published Date

  • Mar 27, 2024

Antagonistic Activity of Cellulase-Producing Yeasts Isolated from Cocoa Fermentation Against Pathogenic Molds Collected from Damaged Cocoa Fruits

KnE Life Sciences / International Conference On Mathematics And Science Education (ICMScE 2022): Life Sciences / Pages 85–96

Abstract

The presence of pathogenic molds in post-harvested cocoa beans poses a significant problem to most Indonesian farmers. Pathogenic molds secrete phytopathogenic toxins which cause rotting, seriously damaging the cocoa fruits. The common pathogenic molds found in damaged cocoa beans are Phytophthora palmivora and Phytophthora megakarya. These molds are so far controlled by using chemical pesticides, whose long-term use is potentially harmful to human health and the environment. Thus, a healthier and safer alternative is needed, especially using biological agents such as yeasts. Some yeasts produce cellulose-degrading enzymes that can possibly break down the fungal cell wall which is composed mostly of the polysaccharide’s chitin and glucan. In this study, yeasts were isolated during the cocoa fermentation process, followed by screening for cellulolytic activity using direct planting and paste method, and subsequent antagonistic testing by double culture technique. Out of 128 yeast isolates, 77 possessed cellulase activity, with 6 of them having the highest activity index. Antagonistic activity test of these 6 isolates (C1.0.4, C1.1.3, C2.3.10, C2.3.14, C3.5.11, and C3.3.1) against the pathogenic molds resulted in isolate C3.3.1 showing the highest inhibition percentage from the 2

References
[1] Nigam PS, Singh A. Cocoa and coffee fermentations. In: Doyle MP, Beuchat RL, editors. Encyclopedia of Food Microbiology. Washington (DC): Elsevier; 2014. pp. 485–92.

[2] Schwan RF, Wheals AE. The microbiology of cocoa fermentation and its role in chocolate quality. Crit Rev Food Sci Nutr. 2004;44(4):205–21.

[3] Susijahadi WT, Unus AS, Jinap S. “Effect of frequency turning on acetic acid concentration and colour, during Cocoa Beans fermentation.,” In: Procc. Malaysian International Cocoa Conterence, Kuala Lumpur 1998., Malaysia (1998).

[4] Ardhana MM, Fleet GH. The microbial ecology of cocoa bean fermentations in Indonesia. Int J Food Microbiol. 2003 Sep;86(1-2):87–99.

[5] De Vuyst L, Weckx S. The functional role of lactic acid bacteria in cocoa bean fermentation. In: Mozzi F, Raya RR, Vignolo GM, editors. Biotechnology of Lactic Acid Bacteria. Chichester, UK: John Wiley & Sons, Ltd; 2015. pp. 248–78.

[6] Copetti MV, Iamanaka BT, Pitt JI, Taniwaki MH. Fungi and mycotoxins in cocoa: from farm to chocolate. Int J Food Microbiol. 2014 May;178:13–20.

[7] Semangun H. Penyakit-penyakit tanaman perkebunan di Indonesia. Yogyakarta: Gadjah Mada University Press; 2020.

[8] Hanada RE, Pomella AW, Soberanis W, Loguercio LL, Pereira JO. Biocontrol potential of Trichoderma martiale against the black-pod disease (Phytophthora palmivora) of cacao. Biol Control. 2009;50(2):143–9.

[9] Sulistyowati E, Junianto YD, Sri-Sukamto W. W. R., L. Primawati, and N., Analisis status penelitian dan pengembangan PHT pada pertanaman Kakao. Risalah Simposium Nasional Penelitian PHT Perkebunan Rakyat Bogor; 2003.

[10] Dharmaputra OS, Sunjaya R. I., and S. Ambarwati, “Stored Cocoa beans quality affected by fermentation and Ephestia cautella Walker (Lepidoptera: Phycitidae) infestation.,”. Biotropia (Bogor). 2000;15(15):58–75.

[11] Nguyen PA, Strub C, Fontana A, Schorr-Galindo S. Crop molds and mycotoxins: alternative management using biocontrol. Biol Control. 2017;104:10–27.

[12] Pimenta RS, Morais PB, Rosa CA, Corrêa A. “Utilization of yeasts in biological control programs.,” in: yeast biotechnology: diversity and applications. pp. 199–214. Springer Netherlands, Dordrecht (2009). https://doi.org/10.1007/978-1-4020-8292- 4_10.

[13] Spadaro D, Droby S. Development of biocontrol products for postharvest diseases of fruit: the importance of elucidating the mechanisms of action of yeast antagonists. Trends Food Sci Technol. 2016;47:39–49.

[14] Madigan MT, Martinko JM, Stahl DA, Clark DP. Brock biology of Microorganisms. Boston: Pearson Education, Inc; 2012.

[15] Sukmawati D. Antagonism mechanism of fungal contamination animal feed using phylloplane yeasts isolated from the Bintaro Plant (Cerbera manghas) Bekasi in Java, Indonesia. Int J Curr Microbiol Appl Sci. 2016;5(5):63–74.

[16] A.E. Puspitasari, A.L. Abadi, and L. Sulistyowati, “Potensi khamir sebagai agens pengendali hayati patogen Colletotrichum Sp. pada Buah Cabai, Buncis, dan Stroberi.,” Jurnal Hama dan Penyakit Tumbuhan. vol. 2, no. 3, p. 92, 2014.

[17] Bajpai VK, Yoon JI, Cho SW, Kang SC. Isolation and morphological characterization of Monilinia sp. KV-27 Associated with Apple Anthracnose of Fuji Apples in Korea. Plant Pathol J. 2010;26(2):185–8.

[18] Sukmawati D, Andrianto MH, Arman Z, Ratnaningtyas NI, Al Husna SN, El-Enshasy HA, et al. Antagonistic activity of phylloplane yeasts from Moringa oleifera Lam. leaves against Aspergillus flavus UNJCC F-30 from chicken feed. Indian Phytopathol. 2020;73(1):79–88.

[19] Cappuccino JG, Sherman N. Microbiology: A Laboratory Manual. San Francisco: Pearson Education Inc; 2002.

[20] Hankin L, Anagnostakis SL. The use of solid media for detection of enzyme production by Fungi. Mycologia. 1975;67(3):597–607.

[21] Hartati S, Wiyono S, Hidayat SH, Sinaga MS. Seleksi khamir epifit sebagai agens antagonis penyakit antraknosa pada cabai. Jurnal Hortikultura. 2016;24(3):258.

[22] Kheireddine A, Essghaier B, Hedi A, Dhieb C, And N. Sadfi-Zouaoui, “New epiphytic yeasts able to reduce grey mold disease on apples.,”. Plant Prot Sci. 2018;54(4):248– 57.

[23] Nakagawa A, Kitahara K; A. NAKAGAWA And K. KITAHARA. Taxonomic studies on the Genus Pediococcus. J Gen Appl Microbiol. 1959;5(3):95–126.

[24] Kurtzman CP, Fell JW. The yeast: A taxonomic study. Amsterdam: Elsevier; 1998.

[25] Sukmawati D, Dellanerra D, Risandi A. Screening the capabilities of Indonesian indigenous mold in producing cellulase enzyme. IOP Conf Series Mater Sci Eng. 2018;434(1):012125.

[26] Wulandari TP, Sukmawati D, Kurniati TH. Isolasi dan seleksi khamir amilolitik asal buah Nangka (Artocarpus heterophyllus Lam.). BIOMA. 2018;13(1):37–42.

[27] Sukmawati D, Arman Z, Sondana GA, Fikriyah NN, Hasanah R, Afifah ZN, et al. Potential amylase-producing yeast isolated from indigenous fermented beverages originating from Bali, Indonesia. J Phys Conf Ser. 2019;1402(5):055021.