The Effect of Adding Cinnamon Bark Oil to the Tyrosinase Inhibitory Activity of Emulgel Containing Cocoa Pod Husk Extract


Our previous study showed that cocoa pod husk extract has tyrosinase inhibitory properties and the potential to prevent skin hyperpigmentation. Another natural source that is known to have tyrosinase inhibitory properties is cinnamon bark oil. This paper aims to analyze the tyrosinase inhibitory properties of cinnamon bark oil through molecular docking. It also aims to determine the effect of adding cinnamon bark oil to the tyrosinase inhibitory properties of emulgel containing cocoa pod husk extract. The constituents of cinnamon bark oil were determined using gas chromatography-mass spectrometry. The molecular docking was conducted using autodock. The emulgels were prepared by adding 2% of cocoa pod husk extract with and without the addition of cinnamon bark oil (1%). Tyrosinase inhibitory properties were analyzed using a colorimetric enzymatic assay and the dopachrome method. The GCMS result showed the cinnamon bark oil containing 53.37% cinnamaldehyde. The in-silico study showed cinnamaldehyde properties as a tyrosinase inhibitor, since it can bind on the active site of the enzyme with free binding energy at -4.88 kcal/mol. The addition of cinnamon bark oil (1%) to the emulgel preparation increased the tyrosinase inhibitory activity by 63.33% based on in vitro study.

Keywords: emulgel, cocoa pod husk extract, cinnamon bar oil, tyrosinase inhibitor

[1] Nieuweboer-Krobotova L. Hyperpigmentation: types, diagnostics and targeted treatment options. J Eur Acad Dermatol Venereol. 2013 Jan;27 Suppl 1:2–4.

[2] Desai SR. Hyperpigmentation therapy: a review. J Clin Aesthet Dermatol. 2014 Aug;7(8):13–7.

[3] D’Mello SA, Finlay GJ, Baguley BC, Askarian-Amiri ME. Signaling Pathways in Melanogenesis. Int J Mol Sci. 2016 Jul;17(7).

[4] Zolghadri S, Bahrami A, Hassan Khan MT, Munoz-Munoz J, Garcia-Molina F, Garcia- Canovas F, et al. A comprehensive review on tyrosinase inhibitors. J Enzyme Inhib Med Chem. 2019 Dec;34(1):279–309.

[5] Priani SE, Aprilia S, Aryani R, Purwanti L. Antioxidant and tyrosinase inhibitory activity of face serum containing cocoa pod husk phytosome (Theobroma cacao L.). J Appl Pharm Sci. 2019;9(10):110–5.

[6] Karim AA, Azlan A, Ismail A, Hashim P, Abd Gani SS, Zainudin BH, et al. BMC Complement Altern Med. 2014;14(1):1–13.

[7] Priani SE, Humanisya H, Darusman F. Int J Sci Res. 2014;3(13):2338–2339.

[8] Sreevidya VS. Journal of Pharmaceutical and Phytopharmacological Research. 2019;9(1):92–97.

[9] Chang CT, Chang WL, Hsu JC, Shih Y, Chou ST. Chemical composition and tyrosinase inhibitory activity of Cinnamomum cassia essential oil. Bot Stud (Taipei, Taiwan). 2013;54(1):1–7.

[10] Senol FS, Khan MT, Orhan G, Gurkas E, Orhan IE, Oztekin NS, et al. In silico approach to inhibition of tyrosinase by ascorbic acid using molecular docking simulations. Curr Top Med Chem. 2014;14(12):1469–72.

[11] Mohamed MI. Optimization of chlorphenesin emulgel formulation. AAPS J. 2004 Oct;6(3).

[12] Priani SE, Lestari IA, Lestari F. Jurnal Ilmiah Ibnu Sina. 2019;4(1):66–76.

[13] Batubara I, Darusman LK, Mitsunaga T, Rahminiwati M, Djauhari E. J Biol Sci. 2010.

[14] Asadzadeh A, Fassihi A, Yaghmaei P, Pourfarzam M. Biotech. Res. Asia. 2015;12.