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Albakri, M., Anugratama, L., ., P., & Hartatik, T. (2022). Identification of Gene Diversity of Melanocortin-4 Receptor and its Relationship with the Birth and Body Size of Beef Cattle. KnE Life Sciences, 453–464. https://doi.org/10.18502/kls.v0i0.11831

https://doi.org/10.18502/kls.v0i0.11831

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authors

  • MW Albakri

    MW Albakri

    Department of Animal Breeding and Genetic, Faculty of Animal Science, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia 55281
  • LE Anugratama

    LE Anugratama

    Department of Animal Breeding and Genetic, Faculty of Animal Science, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia 55281
  • Panjono .

    Panjono .

    Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia 55281
  • T Hartatik

    T Hartatik

    Department of Animal Breeding and Genetic, Faculty of Animal Science, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia 55281

Published Date

  • Sep 13, 2022

Identification of Gene Diversity of Melanocortin-4 Receptor and its Relationship with the Birth and Body Size of Beef Cattle

KnE Life Sciences / The 3rd International Conference on Advance & Scientific Innovation (ICASI) – Life Sciences Chapter / Pages 453–464

Abstract

Melanocortin-4 Receptor (MC4R) expressed in hypothalamus which is part of the brain that are involved in regulation of appetite, metabolism and bodyweight. This study aimed to identify the MC4R gene diversity in the second cross generation of Belgian Blue, Wagyu, and Brahman crossbred and its relationship with the body weight and size birth. Polymerase Chain Reaction (PCR) and DNA sequencing were used to identify Single Nucleotide Polymorphism (SNP). The result of this study showed that MC4R genes was polymorphic with one SNP at 1133 C>G and included missense mutation. SNP 1133 C>G has two of allels C and G with three genotypes. Test of Hardy Weinberg equilibrium showed that the population of cattle was in equilibrium. The heterozigosity values showed that the population of cattle had a low diversity of genetics. The result of association showed that SNP 1133 C>G in MC4R Genes was not able to indicate the difference between the weight, body length, heart girth and wither’s height at birth. Based on this study, it can be concluded that the polymorphism of MC4R genes can only be used to distinguish cattle genotypes but cannot be used as the selection tools for body weight and size birth.


Keywords: Crossbreed beef cattle, Diversity, Heterozigosity, MC4R gene, SNP.

References
[1] Statistik BP. 2017. Kajian Konsumsi Bahan Pokok Tahun 2017.

[2] Putra WPB. 2017. Teknik Persilangan Pada Sapi Belgian Blue (Bos Taurus) Untuk Menghasilkan Bibit Unggul Di Indonesia. BioTrends 8 : 1–4.

[3] Ratna MP. 2018. Penerimaan wagyu sebagai shoku bunka jepang di Indonesia. Humanika 24: 110–7.

[4] Kong HS, J Seong, DS Suh, KD Park and HK Lee. 2012. Identification and analysis of MC4R polymorphisms and their association with economic traits of Korean cattle (Hanwoo). Mol. Biol. Rep. 39:3597–601.

[5] Maharani D, A Fathoni, Sumadi, T Hartatik and M Khusnudin. 2018. Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle. J. Indones. Trop. Anim. Agric. 43:87–93.

[6] Prihandini PW. 2018. Analisis potensi genetik sapi Madura di Kabupaten Pamekasan dan Loka Penelitian Sapi Potong Jawa Timur. Universitas Gadjah Mada.

[7] Latifah. 2019. Deteksi Single Nucleotide Polymorphism dan Hubungan Polymorphism Gen Melanocortin 4 Receptor Terhadap Sifat Pertumbuhan dan Feed Intake pada Kambing Bligon. Universitas Gadjah Mada.

[8] Kurniawati S and NS Hartati. 2018. Optimasi suhu annealing primer degenerate untuk mengamplifikasi fragmen gen arginine decarboxylase ( adc ) genom ubi kayu lokal maluku tenggara. J. ILMU DASAR 19:135–142.

[9] Fathoni A. 2016. Estimasi parameter genetik sifat pertumbuhan dan identifikasi gen MC4R sapi peranakan ongole di Kabupaten Kebumen Jawa Tengah. Universitas Gadjah Mada.

[10] Liu W B, SR Chen, J X Zheng, L J Qu, G Y Xu and N Yang. 2010. Developmental phenotypic-genotypic associations of tyrosinase and melanocortin 1 receptor genes with changing profiles in chicken plumage pigmentation. Poult. Sci. 89: 1110–1114.

[11] Marson E P, JBS Ferraz, FV Meirelles, JCC Balieiro, JP Eler, LGG Figueiredo and GB Mourão. 2005. Genetic characterization of European-Zebu composite bovine using RFLP markers. Genet. Mol. Res. 4:496–505.

[12] Harjosubroto W. 1994. Aplikasi Pemuliaan Ternak Di Lapangan . Gasino, Yogyakarta.

[13] Nei M. 1987. Molecular evolutionary genetics. Columbia University Press, New York.

[14] Javanmard A, MHB Asadzadeh, and Tavakolian J 2005 The allele and genotype frequencies of bovine pituitary-specific transcription factor and leptin genes in Iranian cattle and buffalo populations using PCR-RFLP Iran. J. Biotechnol. 3:104–8

[15] Machado M A, Schuster I, Martinez M L and Campos A L 2003 Genetic Diversity of Four Cattle Breeds Using Microsatellite Markers. Rev. Bras. Zootec. 32:93–98.

[16] Nasuha. 2020. Estimasi Output Sapi Potong Dan Identifikasi Gen MC4R Sapi Peranakan Ongole Di Kabupaten Tuban Provinsi Jawa Timur. Universitas Gadjah Mada, Yogyakarta