Blood Protein Profile of Sentul Chicken with Lactic Acid as an Acidifier in Rations Containing Probiotics
Abstract
The study aimed to evaluate the use of lactic acid as an acidifier in ration containing probiotics on blood protein profile of Sentul chicken. Sixty female Sentul chickens aged 6 months were reared for two months in a 20-unit battery cage and subjected to a completely randomized design (CRD) with four treatments and five replicates, with 3 females each for each unit. The treatments were R0 : Basal ration (BS); R1 : BS + 0.5% lactic acid; R2 : BS + 1.0% lactic acid; and R3 : BS + 1.5% lactic acid. Data were analyzed by Analysis of Variance (ANOVA) using an IBM SPSS Statistics ver. 22. The result showed that lactic acid as an acidifier in rations containing probiotics on blood protein profile of Sentul chicken did not significantly (P>0,05) affected the blood protein profile (uric acid, creatinine and blood urea nitrogen) of Sentul chicken. The average of uric acid as 5.6±1.86 mg/dl; creatinine 1.07±0.40 mg/dl and blood urea nitrogen 4.31±1.08 mg/dl. The conclusion was that the use of up to 1.5% lactic acid as an acidifier in feed created similar results in uric acid, creatinine and blood urea nitrogen.
Keywords: Sentul chicken, blood protein, acidifier
References
[1] Zainal, H., T. Sartika, D. Zainuddin dan Komarudin. 2012. Persilangan pada Ayam Lokal (KUB, Sentul, Gaok) untuk Meningkatkan Produksi Daging Unggas Indonesia. Workshop National Unggas Lokal.
[2] Iriyanti, N. dan B. Hartoyo. 2019. Kualitas Fisik dan Kimiawi Telur Ayam Sentul dengan Pemberian Fermehebafit-Encapsulasi Sebagai Feed Additive Alami. Prosiding Seminar Nasional dan Call for Papers. Fakultas Peternakan, Universitas Jenderal Soedirman. Purwokerto.
[3] Pratama, A.S., T. Yudiarti dan Isroli. 2019. Penambahan Air Perasan Jeruk Nipis (Citrus aurantiifolia) Sebagai Acidifier Terhadap Bobot Relatif, Panjang Relatif Usus Halus dan Konsumsi Pakan Ayam Broiler. Mediagro, 15(1): 32–37.
[4] Hartoyo, B., N. Iriyanti dan E.A. Rimbawanto. 2020. Fungsi Hati dan Kadar Glukosa Darah Ayam Broiler dengan Pemberian Berbagai Jenis Acidifier Sebagai Feed Additive dalam Pakan yang Mengandung Probiotik. Prosiding Seminar Teknologi dan Agribisnis Peternakan VII. Fakultas Peternakan, Universitas Jenderal Soedirman. Purwokerto.
[5] Isroli., T. Yudiarti, Sugiharto, E. Widiastuti, H.I. Wahyuni dan T.A. Sartono. 2015. Pengaruh Penggunaan Tepung Rumput Laut (Gracilaria verrucosa) dan Pare (Momordica charantia) dalam Ransum Terhadap Konsumsi dan Kadar Metabolit Protein Darah Ayam Broiler. Proceedings of Seminar Nasional Peternakan Berkelanjutan 7. Padjadjaran University. Sumedang: 264–268.
[6] Yunarto, N. 2013. Efek Ekstrak Air dan Heksan Herba Suruhan Peperomia Pellucida (L) Kunth Terhadap Penurunan Kadar Asam Urat Serum Darah Ayam Kampung Jantan. Media Litbangkes, 23(1): 8–14.
[7] Suci, D.M., Asella, L.W. Utami dan W. Hermana. 2018. Pengaruh Pemberian Ransum Mengandung Tepung Daun Mengkudu (Morinda citrifolia Linn) Terhadap Performa dan Profil Darah Itik Lokal Periode Grower. Buletin Makanan Ternak, 16(1): 11–23.
[8] Iriyanti, N., B. Hartoyo and E.A. Rimbawanto. 2020. Fungsi Ginjal Ayam Broiler dengan Pemberian Berbagai Jenis Acidifier Sebagai Feed Additive dalam Pakan yang Mengandung Probiotik. Prosiding Seminar Teknologi dan Agribisnis Peternakan VII: Prospek Peternakan di Era Normal Baru Pasca Pandemi COVID-19. Jenderal Soedirman University. Purwokerto: 2–6.
[9] Saili, T., R. Aka, F.A. Auza, W.L. Salido dan A.M. Sari. 2019. Kolesterol, Asam Urat, dan Glukosa Darah Ayam Buras yang Diberi Pakan dengan Ransum Herbal dan Ekstrak Kerang Bakau (Polymesoda erosa). Jurnal Ilmu dan Teknologi Peternakan Tropis, 6(2): 225-231.
[10] El-Katcha, M.I., M.A. Soltan, H.F. El-Kaney and R.K. El-Sayed. 2014. Growth Performance, Blood Parameters, Immune Sesponse and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn Without or with Enzyme Supplementation. Alexandria J. Vet. Sci 40: 95–111.
[11] Hyden, M. 2000. Protected Acid Additives. Feed International.
[12] Sulistyoningsih, M. dan R. Rakmawati. 2015. Optimalisasi Feed Additive Herbal dengan Intermittent Lighting untuk Menurunkan Asam Urat dan Kolesterol pada Ayam Broiler. Seminar Nasional Pangan Lokal, Bisnis dan Eko-Industri. Semarang.
[13] Nurazizah, N., A.I. Nabila, L. Adriani, T. Widjastuti dan D. Latipudin. 2020. Kadar Kolesterol, Kreatinin, Urea Darah dan Kolesteril Telur Ayam Sentul dengan Penambahan Ekstrak Buah Mengkudu yang Disuplementasi Cu dan Zn. Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan, 2(1): 9-18. doi: 10.24198/jnttip.v2i1.25833.
[14] Ritchie, B.W., G.J. Harrison and L.R. Harrison. 1994. Avian Medicine, Principles and Application (Chapter 11: Biochemistries). Florida: Wingers Publishing Inc.
[15] Pratiwi, D.G.M., I.W. Utama dan L.B.K. Ardana. 2012. Kadar Kreatinin Serum Ayam Pedaging Betina yang Diinjeksi dengan Kombinasi Tylosin dan Gentamisin. Indonesia Medicus Veterinus, 1(1): 102-113.
[16] Kerr, M.G. 2002. Veterinary Laboratory Medicine, Clinical Biochemistry and Hematology. 2nd ed. Blackwell Scientific Publications. London. 101-110.
[17] Widhyari, S.D., A. Esfandiari dan A.D. Cahyono. 2015. Profil Kreatinin dan Nitrogen Urea Darah pada Anak Sapi Frisian Holstein yang Disuplementasi Zn. Acta Veterinaria Indonesiana, 2(2): 45-50.
[18] Howell, S.F. 1939. The Determination of The Urea in Chicken Blood. Journal Biol Chem. 128(131): 573-578.
[19] Asella. 2017. Pemberian Tepung Daun Mengkudu (Morinda citrifolia Linn) Terhadap Profil Darah, Kadar Kreatinin dan Urea Nitrogen Darah Itik Lokal Jantan Umur 12 Minggu. Skripsi. Fakultas Peternakan. Institur Pertanian Bogor. Bogor.
[20] Pemayun, I.G.A.G.P. 2002. Evaluation of Nephrotomy Without Sutures in Dog. Journal Veteriner, 3(2): 94-96.
[21] Meyer, D.J. and J. Harvey. 2003. Interpretation and Diagnosis. 2nd Ed. WB. Saunders. Philadelphia.