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Muchtaromah, B., Ahmad, M., S, E. K., A, Y. M., & A, V. L. (2017). Phytochemicals, Antioxidant and Antifungal Properties of Acorus calamus, Curcuma mangga, and Allium sativum. KnE Life Sciences, 3(6), 93-104. https://doi.org/10.18502/kls.v3i6.1119

https://doi.org/10.18502/kls.v3i6.1119

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authors

  • Bayyinatul Muchtaromah

    Bayyinatul Muchtaromah

    Biology Department, Faculty of Science and Technology State Islamic University of Malang. Jl. Gajayana No.50, Malang
  • Mujahidin Ahmad

    Mujahidin Ahmad

    Biology Department, Faculty of Science and Technology State Islamic University of Malang. Jl. Gajayana No.50, Malang
  • Emy Koestanti S

    Emy Koestanti S

    Faculty of Veterinary Medicine. Airlangga University. Jalan Mulyorejo, Surabaya
  • Yuni Ma’rifatul A

    Yuni Ma’rifatul A

    Biology Department, Faculty of Science and Technology State Islamic University of Malang. Jl. Gajayana No.50, Malang
  • Velayati Labone A

    Velayati Labone A

    Biology Department, Faculty of Science and Technology State Islamic University of Malang. Jl. Gajayana No.50, Malang

Published Date

  • Dec 3, 2017

Phytochemicals, Antioxidant and Antifungal Properties of Acorus calamus, Curcuma mangga, and Allium sativum

KnE Life Sciences / The Veterinary Medicine International Conference (VMIC) / Pages 93-104

Abstract

The purpose of this study to determine the content of phytochemicals, antioxidant and antifungal properties of the combination of Acorus calamus, Curcuma mangga, and Allium sativum. This research was descriptive qualitative, extractions were done by maceration method with ethanol with 3 different combinations (C1, C2 and C3). Phytochemical test reagent included 4 kinds of test, namely: alkaloids, flavonoids, triterpenoids, saponins and tannins. As for the antioxidant test, the method used was DPPH. The concentration used at 25, 50, 100, 200, and 400 ppm. As for the antifungal test conducted on Candida albicans with Kirby-Bauer disc methods with a concentration of 100%, followed by the MIC and MBC test with a concentration of 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78% and 0.39%. Phytochemical test results indicated the presence of the alkaloids, flavonoids and triterpenoids compounds in 3 different combinations (C1, C2 and C3). The highest antioxidant levels founded in C1 (61.75) followed by C3 (47.94) and the lowest levels founded in C2 (42.76). The antifungal test showed the inhibitory zone against C.  albicans. The highest inhibitory zone was found in C1 at 5.44 ± 1.78 mm (medium category), followed by C2 at  4.08 ± 0.86 mm (medium category), and C3 at 3.05 ± 0.23 mm (medium category). As for the minimum inhibitory concentration (MIC) value got on the concentration of 0:39% and minimum fungisidal concentration (MFC) values were at a concentration of 0.78%.   

 

Key word: Acorus calamu, Allium sativum, Curcuma manga, Antioxidant, antifungal, Candida albicans

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