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Tristiana Sukmadewi, D. K., Suharjono, ., Dewi, T. K., Reddy, M., & Antonius, S. (2022). Indole Acetic Acid-Producing and Phosphate-Solubilizing Bacteria From the Rhizosphere of Clove (Syzygium Aromaticum L.) in Bali, Indonesia. KnE Life Sciences, 7(3), 119–129. https://doi.org/10.18502/kls.v7i3.11113

https://doi.org/10.18502/kls.v7i3.11113

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authors

  • Desak Ketut Tristiana Sukmadewi

    Desak Ketut Tristiana Sukmadewi

    Agrotechnology Department, Faculty of Agriculture, Warmadewa University, Indonesia
  • . Suharjono

    . Suharjono

    Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University, Indonesia
  • Tirta Kumala Dewi

    Tirta Kumala Dewi

    Research Center Biology, Indonesia Institute of Science, Cibinong
  • M.S. Reddy

    M.S. Reddy

    Auburn University, USA
  • Sarjiya Antonius

    Sarjiya Antonius

    Research Center Biology, Indonesia Institute of Science, Cibinong

Published Date

  • Jun 7, 2022

Indole Acetic Acid-Producing and Phosphate-Solubilizing Bacteria From the Rhizosphere of Clove (Syzygium Aromaticum L.) in Bali, Indonesia

KnE Life Sciences / The First Asian PGPR Indonesian Chapter International e-Conference 2021 / Pages 119–129

Abstract

Clove plants are routinely fertilized with synthetic fertilizer to increase yield. The use of synthetic fertilizer reduces soil productivity. Biofertilizer can be used as an alternative for increasing soil fertility. The goal of this study was to determine the potency of bacteria isolates capable of producing indole acetic acid (IAA) hormone and solubilizing phosphate, and to identify bacteria species from the rhizosphere of clove plants. Soil samples were collected from the clove plants’ rhizosphere, environmental parameters were measured, the potency of IAA-producing and phosphate-solubilizing bacteria was analyzed, and bacteria were molecularly identified. After 48 hours of incubation, isolate TCKI 5 from Karangasem produced the highest IAA hormone levels (19.64 ppm), and isolate TCBP 6 from Buleleng had the highest index of solubilizing phosphate (1.91). A compatibility test between the three best isolates of IAA hormone-producing and phosphate-solubilizing bacteria revealed that TCKI 5 was able to associate with TCBP 6. Isolate TCKI 5 was identified as Leclercia adecarboxylata C107 with a 99.92% similarity, and isolate TCBP 6 as Burkholderia cepacia GJ8 with a 99.61% similarity.


Keywords: Bacteria, clove, Indole Acetic Acid, phosphate, rhizosphere

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