Hypoglycemic Effects of Polyphenol Complexes from Bilberry Leaves and Fruits Sorbed on Brown Buckwheat Flour -- Experimental Evaluation and Prospects of Use


The effects of plant polyphenols on carbohydrate and/or lipid metabolism disorders have wide experimental and clinical justification; however, their effects are limited due to low bioavailability. Thus, the development of technological approaches enhancing their effectiveness and stability is relevant.The aim of this work was to evaluatein vivothe effects of polyphenols from bilberry leaves and fruits, sorbed on the brown buckwheat flour, on C57Bl/6c mice with carbohydrate and lipid metabolism disorders. We assessed in vivothe effect of a food matrix (FM1: bilberry leaf polyphenols sorbed on brown buckwheat flour) on C57Bl/6c mice with induced carbohydrate and lipid metabolism disorders. The aim of the second experiment was to evaluate the effectiveness of prolonged prophylactic consumption of another food matrix (FM2: billberry fruit polyphenols, sorbed on brown buckwheat flour) by C57Bl/6c micewith induced carbohydrate and lipid metabolism disorders. Technological approaches were developed and pilot batches of the food matrices FM1 and FM2were obtained. According to the in vivo testing, a significant decrease in the glucose levels and normalization of glucose tolerance and insulin sensitivity were found in animals treated with FM1. When assessing the in vivo effects of FM2, the hypoglycemic effect of bilberry fruit polyphenols in the composition of the matrix was established. The results of these studies can be used to justify the testing of the developed matrices in a clinical setting and using them as functional food ingredients for preventative nutrition in cases of carbohydrate metabolism disorders.

Keywords: polyphenols, food matrix, functional food ingredient, carbohydrate metabolism, lipid metabolism

[1] NechaevAP,BagryantsevaOV,TutelyanVA.Food ingredients in the production of modern food products. Moscow: DeLiplus; 2013.

[2] BlinkovIL.Treatment properties of food plants. Kiseleva TL, editor. Moscow: Publishing house FNKECTMDL Roszdrava; 2007.

[3] Bedekar A, Shah K, Koffas M.Natural products for type II diabetes treatment.Advances in Applied Microbiology. 2010;71:21-73. doi: 10.1016/S0065- 2164(10)71002-9

[4] Williamson G.The role of polyphenols in modern nutrition. Nutrition Bulletin. 2017;42(3):226-235. doi:10.1111/nbu.12278

[5] Van Hul M, Cani PD.Targeting carbohydrates and polyphenols for a healthy microbiome and healthy weight. Current Nutrition Reports. 2019;8(4):307-316. doi:10.1007/s13668-019-00281-5

[6] Zaklos-Szyda M, Pawlik N.Japanese quince (Chaenomeles japonica L.) fruit polyphenolic extract modulates carbohydrate metabolism in HepG2 cells via AMP-activated protein kinase. Acta Biochimica Polonica. 2018;65(1):67-78. doi:10.18388/abp.2017_1604

[7] Brglez ME, Knez HM, Škerget M, Knez Ž, Bren U.Polyphenols: Extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules. 2016;21(7):901-911. doi:10.3390/molecules21070901

[8] Denev P., Cíž M., Kratchanova M., Blazheva D.Black chokeberry (Aronia melanocarpa) polyphenols reveal different antioxidant, antimicrobial and neutrophilmodulating activities. Food Chemistry. 2019;284:108-117.

[9] Waskiewicz A, Zujko ME, Szczesniewska D et al.Polyphenols and dietary antioxidant potential, and their relationship with arterial hypertension: A cross-sectional study of the adult population in Poland (WOBASZ II). Advances in Clinical and Experimental Medicine. 2019;28(6):797-806. doi:10.17219/acem/91487

[10] Bohn T. (2014). Dietary factors affecting polyphenol bioavailability.Nutrition Reviews. 2014;72(7):429-52. doi: 10.1111/nure.12114

[11] Han MK. (2003). Epigallocatechin gallate, a constituent of green tea, suppresses cytokine-induced pancreatic beta-cell damage. Experimental & Molecular Medicine. 2003;35(2):136-139. doi: 10.1038/emm.2003.19

[12] Yahfoufi N, Alsadi N, Jambi M, Matar C. (2018). The immunomodulatory and anti-inflammatory role of polyphenols.Nutrients. 2018;10(11):1618-1618. doi:10.3390/nu10111618

[13] Luo G, Li Z, Wang Y et al.Resveratrol protects against titanium particle-induced aseptic loosening through reduction of oxidative stress and inactivation of NF- ?B.Inflammation. 2016;392:775–785. doi:10.1007/s10753-016-0306-6

[14] Mazo VK, Sidorova YS, Shipelin VA, Petrov NA, Kochetkova AA.Polyphenolic plant extracts: Effect on disorders of carbohydrate and lipid metabolism in laboratory rodents.Problems of Endocrinology. 2016;4:38-44.

[15] Tutelyan VA.Plant sources of phytonutrients for specialized foods with antidiabetic effects.Tutelyan VA, Kiseleva TA, Kochetkova AA, editors.Moscow: Biblio-globus; 2016.

[16] Lewandowska U, Szewczyk K, Hrabec E, Janecka A, Gorlach S.Overview of metabolism and bioavailability enhancement of polyphenols.Journal of Agriculturaland Food Chemistry. 2013;61(50):12183–12199. doi:10.1021/jf404439b

[17] ZhogovaAA, PerovaIB, SamylinaIAetal.Identification and quantification of the main biologically active substances of motherwort herb using HPLC mass spectrometry. Himiko-PharmatsevticheskiyZhurnal. 2014;48(7):54-59.

[18] Petrov NA, Sidorova YS, Sarkisyan VA et al.Complex of polyphenols sorbed on buckwheat flour as a functional food ingredient. Food and Raw Materials. 2018;6(2):334-341. doi:10.21603/2308-4057-2018-2-334-341

[19] Phillips PJ.Oral glucose tolerance testing. Australian Family Physician. 2012;41(6):391-393.

[20] Argirion I, Weinstein SJ, Männistö S, Albanes D, Mondul AM.Serum insulin, glucose, indices of insulin resistance, and risk of lung cancer. Cancer Epidemiology Biomarkers& Prevention. 2017;26(10):1519-1524. doi:10.1158/1055-9965.EPI-17-0293

[21] Castanheira L, Ferreira MF, Sebastião AM, Telles-Correia D. (2018). Anxiety assessment in pre-clinical tests and in clinical trials: A critical review.Current Topics in MedicinalChemistry. 2018;18(19):1656-1676. doi: 10.2174/1568026618666181115102518

[22] Haddadi H, Rajaei Z, Alaei H, Shahidani S. (2018). Chronic treatment with carvacrol improves passive avoidance memory in a rat model of Parkinson’s disease. Arquivos de Neuro-Psiquiatria. 2018;76(2):71–77. doi:10.1590/0004-282X20170193

[23] Li H, Park H.-M, Ji H.-S et al. Phenolic-enriched blueberry leaf extract attenuates glucose homeostasis, pancreatic ß-cell function, and insulin sensitivity in high-fat diet-induced diabetic mice. Nutrition Research. 2020;73:83-96. doi: 10.1016/j.nutres.2019.09.005

[24] Panickar KS. Effects of dietary polyphenols on neuroregulatory factors and pathways that mediate food intake and energy regulation in obesity.Molecular Nutrition & Food Research. 2013;57(1):34–47. doi:10.1002/mnfr.201200431

[25] Rubio-Ruiz ME, Guarner-Lans V, Cano-Martínez A et al.Resveratrol and quercetin administration improves antioxidant defenses and reduces fatty liver in metabolic syndrome rats. Molecules. 2019;24(7):1297-1297. doi:10.3390/molecules24071297

[26] Kubow S, Hobson L, Iskandar MM, Sabally K, Donnelly DJ, Agellon LB. Extract of Irish potatoes (Solanum tuberosum L.) decreases body weight gain and adiposity and improves glucose control in the mouse model of diet-induced obesity.Molecular Nutrition & Food Research. 2014;58(11):2235–2238. doi:10.1002/mnfr.201400013

[27] PetrovNA, SidorovaYS, Perova IB, Kochetkova AA, Mazo VK. A complex of blueberry polyphenols sorbed on buckwheat flour as a functional food ingredient. VoprosyPitaniya. 2019;88(6):68-72. doi: 10.24411/0042-8833-2019-10066

[28] Roopchand DE, Kuhn P, Rojo LE, Lila MA, Raskin I.Blueberry polyphenol-enriched soybean flour reduces hyperglycemia, body weight gain and serum cholesterol in mice.Pharmacological Research. 2013;68(1):59-67. doi:10.1016/j.phrs.2012.11.008

[29] Li Q, Wang C, Liu F, Hu T, Shen W, Li E, Liao S, Zou Y. (2020). Mulberry leaf polyphenols attenuated postprandial glucose absorption via inhibition of disaccharidases activity and glucose transport in caco-2 cells. Food&Function. 2020;11(2):1835-1844. doi: 10.1039/c9fo01345h