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Zafari, J., Vazini, H., Javani Jouni, F., Abdolmaleki, P., Monajemi, R., Shams, E., & Satari, M. (2019). Anticancer Effects of Moderate Static Magnetic Field on Cancer Cells In Vitro. Research in Molecular Medicine (RMM), 6(3). https://doi.org/10.18502/rmm.v6i3.4610
https://doi.org/10.18502/rmm.v6i3.4610
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- Cited by 2 articles
authors
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Jaber Zafari
Jaber Zafari
School of Pharmacy, Toxicology Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
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Hossein Vazini
Hossein Vazini
2Nursing Department, Basic Sciencses faculty, Hamedan Branch, Islamic Azad University, Hamedan, Iran
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Fatemeh Javani Jouni
Fatemeh Javani Jouni
Nursing Department, Basic Sciencses faculty, Hamedan Branch, Islamic Azad University, Hamedan, Iran
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Parviz Abdolmaleki
Parviz Abdolmaleki
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran
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Ramesh Monajemi
Ramesh Monajemi
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
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Elahe Shams
Elahe Shams
Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
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Mohammad Satari
Mohammad Satari
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran
Published Date
- Jun 19, 2019
Anticancer Effects of Moderate Static Magnetic Field on Cancer Cells In Vitro
Abstract
Background: Expansion of the use of magnetic fields in metals, mining, transport, research, and medicine industries has led to a discussion about the effects of magnetic fields and whether their strength is negligible. The aim of this study was to investigate the effects of magnetic field on the viability and proliferation rate of HeLa cells.
Materials and Methods: We studied the effects of magnetic field on the viability, proliferation rate and membrane lipid peroxidation of cells, thus, HeLa cells (cancer cells) and human fibroblast cells (normal cells) were used. Initially, the cells were cultured in DMEM and to determine the impact of the magnetic field, the cells were treated with magnetic field at 4 specific intensity levels (0, 7, 14 and 21 mT) and 2 exposure times (24 h and 48 h). The viability percentage and inhibition of cell proliferation were calculated by MTT assay and Trypan blue staining, respectively.
Results: Lipid peroxidation of the cell membrane was examined by malondialdehyde (MDA) method. As the intensity and exposure time of the static magnetic field (SMF) increased, the viability percentage and proliferation rate decreased and the lipid peroxidation levels increased in the Hela cells.
Conclusion: In this study, we have shown the anticancer effects of static magnetic field and propose a suitable intensity range that can be effective for the treatment of cancer.
References
[1] Aladjadjiyan A. Study of the influence of magnetic field on some biological characteristics of Zea mais. J Cent Eur Agric. 2002;3(2):89-94.
[2] Belyavskaya N. Biological effects due to weak magnetic field on plants. Adv Space Res. 2004;34(7):1566-74.
[3] Tofani S, Barone D, Cintorino M, de Santi MM, Ferrara A, Orlassino R, et al. Static and ELF magnetic fields induce tumor growth inhibition and apoptosis. Bioelectromagnetics. 2001;22(6):419-28.
[4] McLean M, Engström S, Holcomb R. Static magnetic fields for the treatment of pain. Epilepsy & Behavior. 2001;2(3):S74-S80.
[5] Potenza L, Ubaldi L, De Sanctis R, De Bellis R, Cucchiarini L, Dachà M. Effects of a static magnetic field on cell growth and gene expression in Escherichia coli. Mutat Res Genet Toxicol Environ Mutagen. 2004;561(1):53-62.
[6] Teodori L, Grabarek J, Smolewski P, Ghibelli L, Bergamaschi A, De Nicola M, et al. Exposure of cells to static magnetic field accelerates loss of integrity of plasma membrane during apoptosis. Cytometry. 2002;49(3):113-8.
[7] Saito K, Suzuki H, Suzuki K. Teratogenic effects of static magnetic field on mouse fetuses. Repord Toxical. 2006;22(1):118-24.
[8] Leszczynski D. Rapporteur report: cellular, animal and epidemiological studies of the effects of static magnetic fields relevant to human health. Progress in Biophysics and Molecular Biology. 2005;87(2):247-53.
[9] Aldinucci C, Garcia JB, Palmi M, Sgaragli G, Benocci A, Meini A, et al. The effect of exposure to high flux density static and pulsed magnetic fields on lymphocyte function. Bioelectromagnetics. 2003;24(6):373-9.
[10] Liburdy R, Callahan D, Harland J, Dunham E, Sloma T, Yaswen P. Experimental evidence for 60 Hz magnetic fields operating through the signal transduction cascade: effects on calcium influx and c-MYC mRNA induction. FEBS Lett. 1993;334(3):301-8.
[11] Pipkin J, Hinson W, Young J, Rowland K, Shaddock J, Tolleson W, et al. Induction of stress proteins by electromagnetic fields in cultured HL-60 cells. Bioelectromagnetics. 1999;20(6):347-57.
[12] Sahebjamei H, Abdolmaleki P, Ghanati F. Effects of magnetic field on the antioxidant enzyme activities of suspension−cultured tobacco cells. Bioelectromagnetics. 2007;28(1):42-7.
[13] Holcomb RR, Worthington WB, McCullough BA, McLean MJ. Static magnetic field therapy for pain in the abdomen and genitals. Pediatr Neurol. 2000;23(3):261-4.
[14] Laurence JA, French PW, Lindner RA, Mckenzie DR. Biological effects of electromagnetic fields—mechanisms for the effects of pulsed microwave radiation on protein conformation. J Theor Biol. 2000;206(2):291-8.
[15] Pirozzoli M, Marino C, Lovisolo G, Laconi C, Mosiello L, Negroni A. Effects of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line. Bioelectromagnetics. 2003;24(7):510-6.
[16] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin. 2012;62(1):10-29.
[17] Kawakami T, Nakamura Y, Karibe H. Cyclophosphamide-induced morphological changes in dental root development of ICR mice. PloS one. 2015;10(7):e0133256.
[18] Bakhtiary Z, Shahrooz R, Ahmadi A, Zarei L, editors. Evaluation of antioxidant effects of crocin on sperm quality in cyclophosphamide treated adult mice. Vet Res Forum.; 2014.
[19] Yoshikawa T, Tanigawa M, Tanigawa T, Imai A, Hongo H, Kondo M. Enhancement of nitric oxide generation by low frequency electromagnetic field. Pathophysiol. 2000;7(2):131-5.
[20] Fahimirad S, Karimzadeh G, Ghanati F. Cold-induced changes of antioxidant enzymes activity and lipid peroxidation in two canola (Brassica napus L.) cultivars. Journal of Plant Physiology & Breeding. 2013;3(1):1-11.
[21] Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics. Br J Cancer. 1972;26(4):239.
[22] Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495-516.
[23] Tofani S, Barone D, Berardelli M, Berno E, Cintorino M, Foglia L, et al. Static and ELF magnetic fields enhance the in vivo anti-tumor efficacy of cis-platin against lewis lung carcinoma, but not of cyclophosphamide against B 16 melanotic melanoma. Pharmacol Res. 2003;48(1):83-90.
[24] Javani Jouni F, Abdolmaleki P, Movahedin M. Investigation on the effect of static magnetic field up to 15 mT on the viability and proliferation rate of rat bone marrow stem cells. In Vitro Cell Dev Biol Anim. 2013;49:212-9.
[25] Sarvestani AS, Abdolmaleki P, Mowla SJ, Ghanati F, Heshmati E, Tavasoli Z, et al. Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells. Micron. 2010;41(2):101-4.
[26] Zafari J, Javani Jouni F, Abdolmaleki P, Jalali A, Khodayar M. Investigation on the effect of static magnetic field up to 30 mT on viability percent, proliferation rate and IC50 of HeLa and fibroblast cells. Electromagn Biol Med. 2015;34(03):50-4.
[27] Lai H, Singh NP. Magnetic-field-induced DNA strand breaks in brain cells of the rat. Environ Health Perspect. 2004;112(6):687.
[28] Kim J, Ha CS, Lee HJ, Song K. Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells. Biochem Biophys Res Commun. 2010;400(4):739-44.
[29] Tavasoli Z, Abdolmaleki P, Mowla SJ, Ghanati F, Sarvestani AS. Investigation of the effects of static magnetic field on apoptosis in bone marrow stem cells of rat. The Environmentalist. 2009;29(2):220-4.
[30] Ahmadianpour MR, Abdolmaleki P, Mowla SJ, Hosseinkhani S. Static magnetic field of 6 mT induces apoptosis and alters cell cycle in p53 mutant Jurkat cells. Electromagn Biol Med. 2013;32(1):9-19.
[31] Dixon SJ, Stockwell BR. The role of iron and reactive oxygen species in cell death. Nat Chem Biol. 2014;10(1):9-17.
[32] Ghodbane S, Lahbib A, Sakly M, Abdelmelek H. Bioeffects of static magnetic fields: Oxidative stress, genotoxic effects, and cancer studies. Biomed Res Int. 2013;2013.
[33] Usselman RJ, Hill I, Singel DJ, Martino CF. Spin biochemistry modulates reactive oxygen species (ROS) production by radio frequency magnetic fields. PloS One. 2014;9(3):e93065.
[2] Belyavskaya N. Biological effects due to weak magnetic field on plants. Adv Space Res. 2004;34(7):1566-74.
[3] Tofani S, Barone D, Cintorino M, de Santi MM, Ferrara A, Orlassino R, et al. Static and ELF magnetic fields induce tumor growth inhibition and apoptosis. Bioelectromagnetics. 2001;22(6):419-28.
[4] McLean M, Engström S, Holcomb R. Static magnetic fields for the treatment of pain. Epilepsy & Behavior. 2001;2(3):S74-S80.
[5] Potenza L, Ubaldi L, De Sanctis R, De Bellis R, Cucchiarini L, Dachà M. Effects of a static magnetic field on cell growth and gene expression in Escherichia coli. Mutat Res Genet Toxicol Environ Mutagen. 2004;561(1):53-62.
[6] Teodori L, Grabarek J, Smolewski P, Ghibelli L, Bergamaschi A, De Nicola M, et al. Exposure of cells to static magnetic field accelerates loss of integrity of plasma membrane during apoptosis. Cytometry. 2002;49(3):113-8.
[7] Saito K, Suzuki H, Suzuki K. Teratogenic effects of static magnetic field on mouse fetuses. Repord Toxical. 2006;22(1):118-24.
[8] Leszczynski D. Rapporteur report: cellular, animal and epidemiological studies of the effects of static magnetic fields relevant to human health. Progress in Biophysics and Molecular Biology. 2005;87(2):247-53.
[9] Aldinucci C, Garcia JB, Palmi M, Sgaragli G, Benocci A, Meini A, et al. The effect of exposure to high flux density static and pulsed magnetic fields on lymphocyte function. Bioelectromagnetics. 2003;24(6):373-9.
[10] Liburdy R, Callahan D, Harland J, Dunham E, Sloma T, Yaswen P. Experimental evidence for 60 Hz magnetic fields operating through the signal transduction cascade: effects on calcium influx and c-MYC mRNA induction. FEBS Lett. 1993;334(3):301-8.
[11] Pipkin J, Hinson W, Young J, Rowland K, Shaddock J, Tolleson W, et al. Induction of stress proteins by electromagnetic fields in cultured HL-60 cells. Bioelectromagnetics. 1999;20(6):347-57.
[12] Sahebjamei H, Abdolmaleki P, Ghanati F. Effects of magnetic field on the antioxidant enzyme activities of suspension−cultured tobacco cells. Bioelectromagnetics. 2007;28(1):42-7.
[13] Holcomb RR, Worthington WB, McCullough BA, McLean MJ. Static magnetic field therapy for pain in the abdomen and genitals. Pediatr Neurol. 2000;23(3):261-4.
[14] Laurence JA, French PW, Lindner RA, Mckenzie DR. Biological effects of electromagnetic fields—mechanisms for the effects of pulsed microwave radiation on protein conformation. J Theor Biol. 2000;206(2):291-8.
[15] Pirozzoli M, Marino C, Lovisolo G, Laconi C, Mosiello L, Negroni A. Effects of 50 Hz electromagnetic field exposure on apoptosis and differentiation in a neuroblastoma cell line. Bioelectromagnetics. 2003;24(7):510-6.
[16] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin. 2012;62(1):10-29.
[17] Kawakami T, Nakamura Y, Karibe H. Cyclophosphamide-induced morphological changes in dental root development of ICR mice. PloS one. 2015;10(7):e0133256.
[18] Bakhtiary Z, Shahrooz R, Ahmadi A, Zarei L, editors. Evaluation of antioxidant effects of crocin on sperm quality in cyclophosphamide treated adult mice. Vet Res Forum.; 2014.
[19] Yoshikawa T, Tanigawa M, Tanigawa T, Imai A, Hongo H, Kondo M. Enhancement of nitric oxide generation by low frequency electromagnetic field. Pathophysiol. 2000;7(2):131-5.
[20] Fahimirad S, Karimzadeh G, Ghanati F. Cold-induced changes of antioxidant enzymes activity and lipid peroxidation in two canola (Brassica napus L.) cultivars. Journal of Plant Physiology & Breeding. 2013;3(1):1-11.
[21] Kerr JF, Wyllie AH, Currie AR. Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics. Br J Cancer. 1972;26(4):239.
[22] Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495-516.
[23] Tofani S, Barone D, Berardelli M, Berno E, Cintorino M, Foglia L, et al. Static and ELF magnetic fields enhance the in vivo anti-tumor efficacy of cis-platin against lewis lung carcinoma, but not of cyclophosphamide against B 16 melanotic melanoma. Pharmacol Res. 2003;48(1):83-90.
[24] Javani Jouni F, Abdolmaleki P, Movahedin M. Investigation on the effect of static magnetic field up to 15 mT on the viability and proliferation rate of rat bone marrow stem cells. In Vitro Cell Dev Biol Anim. 2013;49:212-9.
[25] Sarvestani AS, Abdolmaleki P, Mowla SJ, Ghanati F, Heshmati E, Tavasoli Z, et al. Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells. Micron. 2010;41(2):101-4.
[26] Zafari J, Javani Jouni F, Abdolmaleki P, Jalali A, Khodayar M. Investigation on the effect of static magnetic field up to 30 mT on viability percent, proliferation rate and IC50 of HeLa and fibroblast cells. Electromagn Biol Med. 2015;34(03):50-4.
[27] Lai H, Singh NP. Magnetic-field-induced DNA strand breaks in brain cells of the rat. Environ Health Perspect. 2004;112(6):687.
[28] Kim J, Ha CS, Lee HJ, Song K. Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells. Biochem Biophys Res Commun. 2010;400(4):739-44.
[29] Tavasoli Z, Abdolmaleki P, Mowla SJ, Ghanati F, Sarvestani AS. Investigation of the effects of static magnetic field on apoptosis in bone marrow stem cells of rat. The Environmentalist. 2009;29(2):220-4.
[30] Ahmadianpour MR, Abdolmaleki P, Mowla SJ, Hosseinkhani S. Static magnetic field of 6 mT induces apoptosis and alters cell cycle in p53 mutant Jurkat cells. Electromagn Biol Med. 2013;32(1):9-19.
[31] Dixon SJ, Stockwell BR. The role of iron and reactive oxygen species in cell death. Nat Chem Biol. 2014;10(1):9-17.
[32] Ghodbane S, Lahbib A, Sakly M, Abdelmelek H. Bioeffects of static magnetic fields: Oxidative stress, genotoxic effects, and cancer studies. Biomed Res Int. 2013;2013.
[33] Usselman RJ, Hill I, Singel DJ, Martino CF. Spin biochemistry modulates reactive oxygen species (ROS) production by radio frequency magnetic fields. PloS One. 2014;9(3):e93065.