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Khalili-Savadkouhi, S., Karimpour Malekshah, A., Mirhoseini, M., Moosazadeh, M., & Shahidi, M. (2019). The Influence of Light on Apoptosis in Preimplantation Mouse Embryos In Vitro. Research in Molecular Medicine (RMM), 6(3). https://doi.org/10.18502/rmm.v6i3.4607
https://doi.org/10.18502/rmm.v6i3.4607
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authors
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Sepideh Khalili-Savadkouhi
Sepideh Khalili-Savadkouhi
Molecular and Cell Biology Research Center, Hemoglobinopathy Institute, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
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Abbasali Karimpour Malekshah
Abbasali Karimpour Malekshah
Molecular and Cell Biology Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran; Department of Anatomy, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
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Mehri Mirhoseini
Mehri Mirhoseini
Molecular and Cell Biology Research Center, Hemoglobinopathy Institue, Mazandaran University of Medical Sciences, Sari, Iran; Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran
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Mahmood Moosazadeh
Mahmood Moosazadeh
Health Sciences Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, Iran
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Maryam Shahidi
Maryam Shahidi
Department of Biophysics and Biochemistry, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Published Date
- Jun 19, 2019
The Influence of Light on Apoptosis in Preimplantation Mouse Embryos In Vitro
Abstract
Background: In vitro culture of mammalian embryos can slow or stop growth completely. This may be due to the medium used, pH, temperature, or light. There is considerable concern about the harmful effect of light in the laboratory environment. Cell number and apoptosis are useful parameters that indicate embryonic development and health. In this study, we assessed these two factors in the blastocyst.
Materials and methods: A total of 128 embryos were extracted from NMRI mice at the 2-cell stage and were divided into 4 groups. The embryos were exposed to light for 0, 5, 15, and 30 min, and then cultured for 96 h. The degree of embryonic development were recorded every 24 h. Furthermore, several morphologically normal blastocysts were evaluated using the TUNEL assay.
Results: There was no significant difference in developmental stages between the experimental and control groups. An evaluation of the percentage of blastomeres and apoptotic cells revealed significant differences among the four groups. The maximum number of apoptotic blastomeres was observed in the group exposed to light for 30 minutes.
Conclusion: Up to thirty minutes of white fluorescent light can induce apoptosis in blastomeres, but it does not prevent embryo development.
References
[1] Nematollahi-mahani SN, Pahang H, Moshkdanian G, Nematollahi-mahani A. Effect of embryonic fibroblast cell co-culture on development of mouse embryos following exposure to visible light. J Assist Reprod Genet. 2009;26(2-3):129-35.
[2] Fernández-Gonzalez R, Moreira P, Bilbao A, Jiménez A, Pérez-Crespo M, Ramírez MA, et al. Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior. Proc Natl Acad Sci USA. 2004;101(16):5880-5.
[3] Khalili-Savadkouhi S, Karimpour Malekshah A, Alizadeh A, Esmaeilnezhad Moghadam A, Shiravi A, Mirhosseini M. The effect of light on mouse preimplantation embryo development in vitro. J Mazandaran Univ Med Sci. 2011;20(80):17-24.
[4] Schultz RM. From egg to embryo: a peripatetic journey. Reproduction. 2005;130(6):825-8.
[5] Pomeroy KO, Reed ML. The effect of light on embryos and embryo culture. J Reprod Stem Cell Biotechnol. 2012;3(2):46-54.
[6] Schultz RM. Of light and mouse embryos: less is more. Proc Natl Acad Sci USA. 2007;104(37):14547-8.
[7] Oh SJ, Gong SP, Lee ST, Lee EJ, Lim JM. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. Fertil Steril. 2007;88(4):1150-7.
[8] Nakahara T, Iwase A, Goto M, Harata T, Suzuki M, Ienaga M, et al. Evaluation of the safety of time-lapse observations for human embryos. J Assist Reprod Genet. 2010;27(2-3):93-6.
[9] Takenaka M, Horiuchi T, Yanagimachi R. Effects of light on development of mammalian zygotes. Proc Natl Acad Sci USA. 2007;104(36):14289-93.
[10] Kitagawa Y, Suzuki K, Yoneda A, Watanabe T. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology. 2004;62(7):1186-97.
[11] Weissman L, de Souza-Pinto NC, Stevnsner T, Bohr V. DNA repair, mitochondria, and neurodegeneration. Neuroscience. 2007;145(4):1318-29.
[12] Mohn F, Schübeler D. Genetics and epigenetics: stability and plasticity during cellular differentiation. Trends Genet. 2009;25(3):129-36.
[13] Boelhauve M, Sinowatz F, Wolf E, Paula-Lopes FoF. Maturation of bovine oocytes in the presence of leptin improves development and reduces apoptosis of in vitroproduced blastocysts. Biol Reprod. 2005;73(4):737-44.
[14] Watson AJ, De Sousa P, Caveney A, Barcroft LC, Natale D, Urquhart J, et al. Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis. Biol Reprod. 2000;62(2):355-64.
[15] Ottosen LD, Hindkjær J, Ingerslev J. Light exposure of the ovum and preimplantation embryo during ART procedures. J Assist Reprod Genet. 2007;24(2-3):99-103.
[16] Dumollard R, Carroll J, Duchen M, Campbell K, Swann K, editors. Mitochondrial function and redox state in mammalian embryos. Semin Cell Dev Biol; 2009: Elsevier.
[17] Hernández-García D, Wood CD, Castro-Obregón S, Covarrubias L. Reactive oxygen species: a radical role in development? Free Rad Biol Med. 2010;49(2):130-43.
[18] Lesser MP, Kruse VA, Barry TM. Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos. J Exp Biol. 2003;206(22):4097-103.
[19] Jaroudi S, SenGupta S. DNA repair in mammalian embryos. Mutat Res Rev Mutat Res. 2007;635(1):53-77.
[20] Zeng F, Baldwin DA, Schultz RM. Transcript profiling during preimplantation mouse
development. Dev Biol. 2004;272(2):483-96.
[21] Artus J, Cohen-Tannoudji M. Cell cycle regulation during early mouse embryogenesis. Mol Cell Endocrinol. 2008;282(1):78-86.
[22] Latchman DS. Heat shock proteins and cardiac protection. Cardiovasc Res.
2001;51(4):637-46.
[23] Sørensen JG, Kristensen TN, Loeschcke V. The evolutionary and ecological role of heat shock proteins. Ecol Lett. 2003;6(11):1025-37.
[2] Fernández-Gonzalez R, Moreira P, Bilbao A, Jiménez A, Pérez-Crespo M, Ramírez MA, et al. Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior. Proc Natl Acad Sci USA. 2004;101(16):5880-5.
[3] Khalili-Savadkouhi S, Karimpour Malekshah A, Alizadeh A, Esmaeilnezhad Moghadam A, Shiravi A, Mirhosseini M. The effect of light on mouse preimplantation embryo development in vitro. J Mazandaran Univ Med Sci. 2011;20(80):17-24.
[4] Schultz RM. From egg to embryo: a peripatetic journey. Reproduction. 2005;130(6):825-8.
[5] Pomeroy KO, Reed ML. The effect of light on embryos and embryo culture. J Reprod Stem Cell Biotechnol. 2012;3(2):46-54.
[6] Schultz RM. Of light and mouse embryos: less is more. Proc Natl Acad Sci USA. 2007;104(37):14547-8.
[7] Oh SJ, Gong SP, Lee ST, Lee EJ, Lim JM. Light intensity and wavelength during embryo manipulation are important factors for maintaining viability of preimplantation embryos in vitro. Fertil Steril. 2007;88(4):1150-7.
[8] Nakahara T, Iwase A, Goto M, Harata T, Suzuki M, Ienaga M, et al. Evaluation of the safety of time-lapse observations for human embryos. J Assist Reprod Genet. 2010;27(2-3):93-6.
[9] Takenaka M, Horiuchi T, Yanagimachi R. Effects of light on development of mammalian zygotes. Proc Natl Acad Sci USA. 2007;104(36):14289-93.
[10] Kitagawa Y, Suzuki K, Yoneda A, Watanabe T. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology. 2004;62(7):1186-97.
[11] Weissman L, de Souza-Pinto NC, Stevnsner T, Bohr V. DNA repair, mitochondria, and neurodegeneration. Neuroscience. 2007;145(4):1318-29.
[12] Mohn F, Schübeler D. Genetics and epigenetics: stability and plasticity during cellular differentiation. Trends Genet. 2009;25(3):129-36.
[13] Boelhauve M, Sinowatz F, Wolf E, Paula-Lopes FoF. Maturation of bovine oocytes in the presence of leptin improves development and reduces apoptosis of in vitroproduced blastocysts. Biol Reprod. 2005;73(4):737-44.
[14] Watson AJ, De Sousa P, Caveney A, Barcroft LC, Natale D, Urquhart J, et al. Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis. Biol Reprod. 2000;62(2):355-64.
[15] Ottosen LD, Hindkjær J, Ingerslev J. Light exposure of the ovum and preimplantation embryo during ART procedures. J Assist Reprod Genet. 2007;24(2-3):99-103.
[16] Dumollard R, Carroll J, Duchen M, Campbell K, Swann K, editors. Mitochondrial function and redox state in mammalian embryos. Semin Cell Dev Biol; 2009: Elsevier.
[17] Hernández-García D, Wood CD, Castro-Obregón S, Covarrubias L. Reactive oxygen species: a radical role in development? Free Rad Biol Med. 2010;49(2):130-43.
[18] Lesser MP, Kruse VA, Barry TM. Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos. J Exp Biol. 2003;206(22):4097-103.
[19] Jaroudi S, SenGupta S. DNA repair in mammalian embryos. Mutat Res Rev Mutat Res. 2007;635(1):53-77.
[20] Zeng F, Baldwin DA, Schultz RM. Transcript profiling during preimplantation mouse
development. Dev Biol. 2004;272(2):483-96.
[21] Artus J, Cohen-Tannoudji M. Cell cycle regulation during early mouse embryogenesis. Mol Cell Endocrinol. 2008;282(1):78-86.
[22] Latchman DS. Heat shock proteins and cardiac protection. Cardiovasc Res.
2001;51(4):637-46.
[23] Sørensen JG, Kristensen TN, Loeschcke V. The evolutionary and ecological role of heat shock proteins. Ecol Lett. 2003;6(11):1025-37.