Download fulltext
HTML
Sheikhalizadeh, V., Rezaee, M. A., Langarizadeh, N., & Goli, H. R. (2019). Different Features of Escherichia coli and Klebsiella pneumoniae in Children and Adults. Research in Molecular Medicine (RMM), 6(1), 30-40. https://doi.org/10.18502/rmm.v6i1.3931
https://doi.org/10.18502/rmm.v6i1.3931
statistics
- 1015 Abstract Views
- 155 PDF Views
- 0 HTML Views
authors
-
Vajihe Sheikhalizadeh
No author data available.
-
Mohammad Ahangarzadeh Rezaee
No author data available.
-
Nastaran Langarizadeh
No author data available.
-
Hamid Reza Goli
No author data available.
Published Date
- Mar 11, 2019
Different Features of Escherichia coli and Klebsiella pneumoniae in Children and Adults
Abstract
Introduction: This study aimed to describe the association between age groups and antimicrobial susceptibility patterns, as well as integron presence in Escherichia coli and Klebsiella pneumoniae isolates from Tabriz, Iran.
Materials and methods: Equal numbers of isolates from adults and children, 140 for E. coli and 150 for K. pneumoniae, were examined for susceptibility to 13 routine antibiotics. Integron existence in multidrug resistant (MDR) isolates was also determined using PCR-RFLP.
Results: Significant age-related differences were observed in the resistance rates of K. pneumoniae toward cotrimoxazol, nalidixic acid, ciprofloxacin, and norfloxacin. For E. coli, age-related differences in the resistance rates to tetracycline, chloramphenicol, ciprofloxacin, and norfloxacin were significant. PCR-RFLP results revealed the presence of class 1 integron (intI1) in 24.5% and 19.2% of MDR E. coli in children and adults, respectively. In K. pneumoniae, 72.9% of isolates from children and 84% from adults were positive for intI1. The prevalence of class 2 integrons was significantly associated with age, in both E. coli and K. pneumoniae. No class 3 integrons were detected in this study.
Conclusions: The resistance rates differ across age groups. Moreover, this study is the first to demonstrate age-related differences in integron presence, especially for class 2 integrons, in E. coli and K. pneumoniae.
References
[1] NNIS System. National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1992 through June 2003. Am J Infec Control. 2003;31:481–98.
[2] Sahm DF, Thornsberry C, Mayfield DC, Jones ME, Karowlosky JA. Multi-drug resistance urinary tract infections of Escherichia coli prevalence and patient demographics in the United States in 2000. Antimicrob Agents Chemother.2001;45(5):1402–06.
[3] Leverstein–van Hall MA, M Blok HE. Donders AR, Paauw A, Fluit AC, Verhoef J. Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. J Infect Dis. 2003;187:251–59.
[4] Cambray G, Guerout AM, Mazel D. Integrons. Ann Rev Genet. 2010;44:141–66.
[5] Carattoli A. Importance of integrons in the diffusion of resistance. Vet Res. 2001;32:243–59.
[6] Gassama A, Aidara-Kane A, Chainier D, Denis F, Poly MC. Integron-associated antibiotic resistance in enteroaggregative and enteroinvasive Escherichia coli. Microb Drug Resist. 2004;10:27–30.
[7] Grape M, Farra A, Kronvall G, Sundström L. Integrons and gene cassettes in clinical isolates of co-trimoxazole-resistant Gram-negative bacteria. Clin Microbiol Infect. 2005;11:185–92.
[8] Fluit AC, Schmitz FJ. Resistance integrons and super-integrons. Clin Microbiol Infect. 2004;10:272–88.
[9] Japoni A, Gudarzi M, Farshad SH, Basiri E, Ziyaeyan M, Alborzi A, et al. Assay for integrons and pattern of antibiotic resistance in clinical Escherichia coli strains by PCR-RFLP in southern Iran. Jpn J Infect Dis. 2008;61(1):85–88.
[10] Lim KT, Yeo CC, Yasin R M, Balan G, Thong KL. Characterization of multidrug-resistant and extended-spectrum β-lactamase- producing Klebsiella pneumoniae strains from Malaysian hospitals. J Med Microbiol. 2009;58:1463–69.
[11] Lim KT, Yasin R, Yeo CC, Puthucheary S, Thong KL. Characterization of multidrugresistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia. J Biomed Biotech. 2009;e165637.
[12] Mathai E, Grape M, Kronval G. Integrons and multidrug resistance among E. coli causing community-acquired urinary tract infection in southern India. APMIS. 2004;12:159–64.
[13] Rao AN, Barlow M, Clark LA, Boring GR, Tenover FJ, McGowan EJ. Class 1 integrons in resistant Escherichia coli and Klebsiella spp., US hospitals. Emerg Infect Dis. 2006;12(6):1011–14.
[14] Goli HR, Nahaei MR, Rezaee MA, Samadi H, Aghazadeh M, Sheikhalizadeh V. Mol Genet, Microbiol, Virol. 2017;32(2):109–15.
[15] Yu HS, Lee JC, Kang HU, Ro DW, Chung JY, Jeong YS, et al. Changes in gene cassettes of class 1 integrons among E. coli isolated from urine specimens collects in Korea during the last two decades. J Clin Microbiol. 2003;41:5429–33.
[16] CLSI. Clinical and Laboratory Standards Institute. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth Informational Supplement. CLSI document.
[17] White PA, McIver CJ, Rawlinson WD. Integrons and gene cassettes in the enterobacteriaceae. Antimicrob Agents Chemother. 2011;45(9):26–61.
[18] Renuart AJ, Goldfarb DM, Mokomane M, Tawanana ET, Narasimhamurthy M, Steenhoff AP, et al. Microbiology of urinary tract infections in Gaborone, Botswana. PLoS ONE. 2013;8(3):e57776.
[19] Oteo J, Campos J, Baquero F. Antibiotic resistance in 1962 invasive isolates of Escherichia coli in 27 Spanish hospitals participating in the European Antimicrobial Resistance Surveillance System (2001). J Antimicrob Chemother. 2002;50(6):945– 52.
[20] Hooper D C. Emerging mechanisms of fluoroquinolone resistance. Emerg Infect Dis.
2001;7(2):337–41.
[21] Tonkic M, Goic-Barisic I, Punda-Polic V. Prevalence and antimicrobial resistance of extended-spectrum β-lactamases-producing E. coli and K. pneumoniae strains isolated in a university hospital in Split, Croatia. Int Microb. 2005;8(2):119–24.
[22] Yildirim M, Sahin I, Gulcan A, Ozdemir D, Kucukbayrak A, Uzun H, et al. Antimicrobial susceptibility and uropathogens isolated from children and adults with community acquired urinary tract infections. Turk Klin J Med Sci. 2010;30(2):533–38.
[23] Farshad S, Japoni A, Hosseini M. Low distribution of integrons among multidrug resistant E. coli strains isolated from children with community-acquired urinary tract infections in Shiraz, Iran. Pol J Microbiol. 2008;57(3):193–98.
[24] Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. Antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary hospital in Tanzania. BMC Res Notes. 2010;3:348.
[25] Kang H Y, Young, SJ, Jae YO, Tae SH, Choi CH, Moon DC, et al. Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli strains from humans and animals in Korea. J Antimicrob Chemother. 2005;55:639–44.
[26] Gundogdu A, Long YB, Vollmerhausen TL, Katouli M. Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia. J Med Microb. 2011;60:163342.
[27] Sepp E, Stsepetova J, Lõivukene K, Truusalu K, Koljalg S, Naaber P, et al. The occurrence of antimicrobial resistance and class 1 integrons among commensal Escherichia coli isolates from infants and elderly persons. Ann Clin Microb Anti.2009;8:34.
[2] Sahm DF, Thornsberry C, Mayfield DC, Jones ME, Karowlosky JA. Multi-drug resistance urinary tract infections of Escherichia coli prevalence and patient demographics in the United States in 2000. Antimicrob Agents Chemother.2001;45(5):1402–06.
[3] Leverstein–van Hall MA, M Blok HE. Donders AR, Paauw A, Fluit AC, Verhoef J. Multidrug resistance among Enterobacteriaceae is strongly associated with the presence of integrons and is independent of species or isolate origin. J Infect Dis. 2003;187:251–59.
[4] Cambray G, Guerout AM, Mazel D. Integrons. Ann Rev Genet. 2010;44:141–66.
[5] Carattoli A. Importance of integrons in the diffusion of resistance. Vet Res. 2001;32:243–59.
[6] Gassama A, Aidara-Kane A, Chainier D, Denis F, Poly MC. Integron-associated antibiotic resistance in enteroaggregative and enteroinvasive Escherichia coli. Microb Drug Resist. 2004;10:27–30.
[7] Grape M, Farra A, Kronvall G, Sundström L. Integrons and gene cassettes in clinical isolates of co-trimoxazole-resistant Gram-negative bacteria. Clin Microbiol Infect. 2005;11:185–92.
[8] Fluit AC, Schmitz FJ. Resistance integrons and super-integrons. Clin Microbiol Infect. 2004;10:272–88.
[9] Japoni A, Gudarzi M, Farshad SH, Basiri E, Ziyaeyan M, Alborzi A, et al. Assay for integrons and pattern of antibiotic resistance in clinical Escherichia coli strains by PCR-RFLP in southern Iran. Jpn J Infect Dis. 2008;61(1):85–88.
[10] Lim KT, Yeo CC, Yasin R M, Balan G, Thong KL. Characterization of multidrug-resistant and extended-spectrum β-lactamase- producing Klebsiella pneumoniae strains from Malaysian hospitals. J Med Microbiol. 2009;58:1463–69.
[11] Lim KT, Yasin R, Yeo CC, Puthucheary S, Thong KL. Characterization of multidrugresistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia. J Biomed Biotech. 2009;e165637.
[12] Mathai E, Grape M, Kronval G. Integrons and multidrug resistance among E. coli causing community-acquired urinary tract infection in southern India. APMIS. 2004;12:159–64.
[13] Rao AN, Barlow M, Clark LA, Boring GR, Tenover FJ, McGowan EJ. Class 1 integrons in resistant Escherichia coli and Klebsiella spp., US hospitals. Emerg Infect Dis. 2006;12(6):1011–14.
[14] Goli HR, Nahaei MR, Rezaee MA, Samadi H, Aghazadeh M, Sheikhalizadeh V. Mol Genet, Microbiol, Virol. 2017;32(2):109–15.
[15] Yu HS, Lee JC, Kang HU, Ro DW, Chung JY, Jeong YS, et al. Changes in gene cassettes of class 1 integrons among E. coli isolated from urine specimens collects in Korea during the last two decades. J Clin Microbiol. 2003;41:5429–33.
[16] CLSI. Clinical and Laboratory Standards Institute. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth Informational Supplement. CLSI document.
[17] White PA, McIver CJ, Rawlinson WD. Integrons and gene cassettes in the enterobacteriaceae. Antimicrob Agents Chemother. 2011;45(9):26–61.
[18] Renuart AJ, Goldfarb DM, Mokomane M, Tawanana ET, Narasimhamurthy M, Steenhoff AP, et al. Microbiology of urinary tract infections in Gaborone, Botswana. PLoS ONE. 2013;8(3):e57776.
[19] Oteo J, Campos J, Baquero F. Antibiotic resistance in 1962 invasive isolates of Escherichia coli in 27 Spanish hospitals participating in the European Antimicrobial Resistance Surveillance System (2001). J Antimicrob Chemother. 2002;50(6):945– 52.
[20] Hooper D C. Emerging mechanisms of fluoroquinolone resistance. Emerg Infect Dis.
2001;7(2):337–41.
[21] Tonkic M, Goic-Barisic I, Punda-Polic V. Prevalence and antimicrobial resistance of extended-spectrum β-lactamases-producing E. coli and K. pneumoniae strains isolated in a university hospital in Split, Croatia. Int Microb. 2005;8(2):119–24.
[22] Yildirim M, Sahin I, Gulcan A, Ozdemir D, Kucukbayrak A, Uzun H, et al. Antimicrobial susceptibility and uropathogens isolated from children and adults with community acquired urinary tract infections. Turk Klin J Med Sci. 2010;30(2):533–38.
[23] Farshad S, Japoni A, Hosseini M. Low distribution of integrons among multidrug resistant E. coli strains isolated from children with community-acquired urinary tract infections in Shiraz, Iran. Pol J Microbiol. 2008;57(3):193–98.
[24] Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. Antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary hospital in Tanzania. BMC Res Notes. 2010;3:348.
[25] Kang H Y, Young, SJ, Jae YO, Tae SH, Choi CH, Moon DC, et al. Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli strains from humans and animals in Korea. J Antimicrob Chemother. 2005;55:639–44.
[26] Gundogdu A, Long YB, Vollmerhausen TL, Katouli M. Antimicrobial resistance and distribution of sul genes and integron-associated intI genes among uropathogenic Escherichia coli in Queensland, Australia. J Med Microb. 2011;60:163342.
[27] Sepp E, Stsepetova J, Lõivukene K, Truusalu K, Koljalg S, Naaber P, et al. The occurrence of antimicrobial resistance and class 1 integrons among commensal Escherichia coli isolates from infants and elderly persons. Ann Clin Microb Anti.2009;8:34.