Download fulltext HTML
Khalili, M., Bayat, M., Hazaveh, S. J. H., & Robati, A. karami. (2019). Subtilisin Genes (SUB1-3) Presence in Microsporum canis Isolates with Human and Animal Source. Research in Molecular Medicine (RMM), 6(1), 54-62. https://doi.org/10.18502/rmm.v6i1.3919

https://doi.org/10.18502/rmm.v6i1.3919

statistics

  • 506 Abstract Views
  • 213 PDF Views
  • 0 HTML Views
  • Cited by 16 articles

authors

  • Mohammad Khalili
    No author data available.
  • Mansour Bayat
    No author data available.
  • Seyed Jamal Hashemi Hazaveh
    No author data available.
  • Azadeh karami Robati
    No author data available.

Published Date

  • Mar 11, 2019

Subtilisin Genes (SUB1-3) Presence in Microsporum canis Isolates with Human and Animal Source

Research in Molecular Medicine (RMM) / Research in Molecular Medicine (RMM): Volume 6, Issue No. 1 / Pages 54-62

Abstract

Background: The presence of subtilisin genes (SUBs) coding for serine proteases in Microsporum canis DNA, contribute to the adherence of fungi to keratinized tissues. The aim of this study was identifed the presence/absence of subtilisin gene family in M.canis isolated from human and animal source.
Materials and Methods: This cross-sectional study was performed on 24 samples of patients and pets with dermatophytosis from September to November 2017. Genomic DNA, pertaining to all pure colonies in Sabouraud agar with cycloheximide and chloramphenicol (SCC) and Sabouraud Dextrose agar(SDA), extracted, using rapid method without liquid nitrogen. M. canis were detected by molecular test. SUB1,
SUB2 and SUB3 gene of M. canis amplified. The relative frequency of the genomic sequences also calculated.
Results: M. canis DNA were extracted from cats (n=6), dog (n=1) and human (n=3) with tinea corporis and showed a high percentage for SUB2 (90%; 9/10) and the absence of SUB genes in a M. canis isolated from dog with develop dermatophytosis. The same results were observed in the frequency of the SUBs presence from the colonies grown on SCC and SDA.
Conclusion: The presence of SUBs in M. canis isolates with human and animal source will be the basal to understand zoonotic infections. The absence SUBs in clinical isolate, indicates that they are indispensable for initiation of the infection.

References
[1] Cabañes F. Animal dermatophytosis. Recent advances. Revista Rev Iberoam Micol.2000; 17(1): S8-12.

[2] Rezaei-Matehkolaei A, Makimura K, de Hoog GS, et al. Multilocus differentiation of the related dermatophytes Microsporum canis, Microsporum ferrugineum and Microsporum audouinii. Medic Microbiol 2012; 61(Pt 1):57-63.

[3] Hermoso de Mendoza M, Hermoso de Mendoza J, Alonso JM, et al. A zoonotic ringworm outbreak caused by a dysgonic strain of Microsporum canis from stray cats. Revista Iberoamericana De Micología. 2010; 27(2): 62-5.

[4] Achterman RR, White TC. Dermatophyte virulence factors: Identifying and analyzing genes that may contribute to chronic or acute skin infections. Int J Microbiol. 2012:358305.

[5] Scott DW, Miller WH, Griffin CE. Muller and Kirk’s Small Animal Dermatology. 6th ed. Philadelphia, PA: WB Saunders; 2001. Structure and function of the skin; 26–27.

[6] Gumral R, Ilkit M. Turkish Journal of Medical Sciences Comparison of the contamination rates of culture media used for isolation and identification of dermatophytes. Turk J Med Sci. 2015; 45(3):587-92.

[7] Turska E, Konopka K, Turski W.The effect of chloramphenicol and cycloheximide on the activity of enzyme ”markers” of mitochondrial substructures of the rat liver. Acta Biol Med Ger. 1977; 36(9):1231-6

[8] Mahler HR, Perlman P, Henson C. Selective effects of chloramphenicol, cycloheximide and nalidixic acid on the biosynthesis of respiratory enzymes in yeast Biochem Biophys Res Commun. 1968; 31(3): 474-80.

[9] Brillowska-Dabrowska A, Michalek E, Lindhardt Saunte DM, et al. PCR test for Microsporum canis identifi cation.. Med Mycol. 2013; 51:576-9.

[10] Baldoa A, Monod M, Mathy A, et al. Mechanisms of skin adherence and invasion by dermatophytes. Mycoses. 2012; 55(3):218-23.