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https://doi.org/10.18502/rmm.v5i4.3065
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Mansoureh Taghizadeh
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Hajieh Ghasemian Safaei
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Farkhondeh Poursina
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Published Date
- Oct 10, 2018
Identification of Lactobacillus plantarum in Breast Milk
Abstract
Introduction: The anti-infective effect of early colonization of infants by potentially probiotic lactic acid bacteria in human milk is a growing area of research. Lactobacillus plantarum colonization in early infancy may be important to health in later life. Here, we present an investigation into the presence of L. plantarum in breast milk from Iranian mothers.
Materials and Methods: Human breast milk samples (n = 40) were randomly collected from lactating and breastfeeding women having undergone full-term pregnancies. Information concerning personal characteristics was collected after birth. The samples were cultured in de Man, Rogosa, and Sharpe medium using the pour plate technique, and isolates were initially identified by biochemical methods. Isolates were established as belonging to the genus Lactobacillus based on the 16- 23S rRNA region, and the species L. plantarum was identified using PCR and primers targeting the recA gene.
Results: In our study, 35 samples (87.5%) contained suspected lactobacilli based on phenotypic tests. Thirty of these (85.71%) were confirmed as containing bacteria of the genus Lactobacillus using a genotypic method (PCR), all of which were found to be L. plantarum.
Conclusion: Probiotic bacteria in a mother’s breast milk may have positive effects on her infant’s health. This insight creates new perspectives concerning the use of breast milk as a source of probiotic bacteria for bacteriotherapy.
References
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[26] De Vries MC, Vaughan EE, Kleerebezem M, de Vos WM. Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract. Int Dairy J. 2006;16(9):1018-28.
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[28] Makino H, Kushiro A, Ishikawa E, Muylaert D, Kubota H, Sakai T, et al. Transmission of intestinal Bifidobacterium longum subsp. longum strains from mother to infant determined by multilocus sequencing typing and amplified fragment length polymorphism. Appl Environ Microbiol. 2011; doi:10.1128. PMID: 21821739
[29] Hugenschmidt S, Schwenninger SM, Gnehm N, Lacroix C. Screening of a natural biodiversity of lactic and propionic acid bacteria for folate and vitamin B12 production in supplemented whey permeate. Int Dairy J. 2010;20(12):852-7.
[30] Paolillo R, Carratelli CR, Sorrentino S, Mazzola N, Rizzo A. Immunomodulatory effects of Lactobacillus plantarum on human colon cancer cells. Int Immunopharmacol. 2009;9(11):1265-71. PMID: 19647100
[31] Yen D, Cheung J, Scheerens H, Poulet F, McClanahan T, Mckenzie B, et al. IL-23 is essential for T cellmediated colitis and promotes inflammation via IL-17 and IL-6. J Clin Invest. 2006;116(5):1310-6. PMID:16670770
[32] Naruszewicz M, Johansson ML, Zapolska-Downar D, Bukowska H. Effect of Lactobacillus plantarum
299v on cardiovascular disease risk factors in smokers. Am J Clin Nutr. 2002;76(6):1249-55. PMID: 12450890
[33] Paineau D, Carcano D, Leyer G, Darquy S, Alyanakian MA, Simoneau G, et al. Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial. FEMS Immunol Med Microbiol. 2008;53(1):107-13. PMID: 18422632
[34] Daniel C, Poiret S, Goudercourt D, Dennin V, Leyer G, Pot B. Selecting lactic acid bacteria for their safety and functionality by use of a mouse colitis model. Appl Environ Microbiol. 2006;72(9):5799-805. PMID: 16957197
[35] K
[2] Petherick A. Development: mother’s milk: a rich opportunity. Nature. 2010;468(7327):S5-S7. PMID:21179083
[3] Walker A. Breast milk as the gold standard for protective nutrients. J Pediatr. 2010;156(2):S3-S7. PMID:20105662
[4] LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, et al. Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk. J Immunol. 2006;176(6):3742-52. PMID: 16517743
[5] Stockinger S, Hornef MW, Chassin C. Establishment of intestinal homeostasis during the neonatal period. Cell Mol Life Sci. 2011;68(22):3699-712. PMID: 21952827
[6] Hoppu U, Isolauri E, Laakso P, Matomäki J, Laitinen K. Probiotics and dietary counselling targeting maternal dietary fat intake modifies breast milk fatty acids and cytokines. Eur J Nutr. 2012;51(2):211-9. PMID: 21626296
[7] Brandtzaeg P. ’ABC’ of mucosal immunology. Nestle Nutr Workshop Ser Pediatr Program. 2009;64:23-
38. PMID: 19710513
[8] Martin R, Langa S, Reviriego C, Jiménez E, Martin ML, Olivares M, et al. The commensal microflora of human milk: new perspectives for food bacteriotherapy and probiotics. Trends Food Sci Technol. 2004;15(3):121-7.
[9] Martín R, Langa S, Reviriego C, Jimínez E, Marín ML, Xaus J, et al. Human milk is a source of lactic acid bacteria for the infant gut. J Pediatr. 2003;143(6):754-8. PMID: 14657823
[10] Martín R, Olivares M, Marín ML, Fernández L, Xaus J, Rodríguez JM. Probiotic potential of 3 lactobacilli strains isolated from breast milk. J Hum Lact. 2005;21(1):8-17. PMID: 15681631
[11] Martín R, Jiménez E, Olivares M, Marín M, Fernández L, Xaus J, et al. Lactobacillus salivarius CECT 5713, a potential probiotic strain isolated from infant feces and breast milk of a mother–child pair. Int J Food Microbiol. 2006;112(1):35-43. PMID: 16843562
[12] Martín V, Maldonado-Barragán A, Moles L, Rodriguez-Baños M, del Campo R, Fernández L, et al. Sharing of bacterial strains between breast milk and infant feces. J Hum Lact. 2012;28(1):36-44. PMID:22267318
[13] Beasley SS, Saris PE. Nisin-producing Lactococcus lactis strains isolated from human milk. Appl Environ Microbiol. 2004;70(8):5051-3. PMCID: PMC492443
[14] Heikkilä M, Saris P. Inhibition of Staphylococcus aureus by the commensal bacteria of human milk. J
Appl Microbiol. 2003;95(3):471-8. PMID: 12911694
[15] Perez PF, Doré J, Leclerc M, Levenez F, Benyacoub J, Serrant P, et al. Bacterial imprinting of the neonatal immune system: lessons from maternal cells? Pediatrics. 2007;119(3):e724-e32. PMID: 17332189
[16] Albesharat R, Ehrmann MA, Korakli M, Yazaji S, Vogel RF. Phenotypic and genotypic analyses of lactic
acid bacteria in local fermented food, breast milk and faeces of mothers and their babies. Syst Appl Microbiol. 2011;34(2):148-55. PMID: 21300508
[17] Heilig HG, Zoetendal EG, Vaughan EE, Marteau P, Akkermans AD, de Vos WM. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol. 2002;68(1):114-23. PMID: 11772617
[18] Sghir A, Gramet G, Suau A, Rochet V, Pochart P, Dore J. Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization. Appl Environ Microbiol. 2000;66(5):2263-6. PMID: 10788414
[19] Sinkiewicz G, Ljunggren L. Occurrence of Lactobacillus reuteri in human breast milk. Microb Ecol Health
Dis. 2008;20(3):122-6.
[20] Saavedra JM, Bauman N, Perman J, Yolken R, Oung I. Feeding of Bifidobacterium bifidum and Streptococcus thermophilus to infants in hospital for prevention of diarrhoea and shedding of rotavirus. Lancet. 1994;344(8929):1046-9. PMID: 7934445
[21] Huang JS, Bousvaros A, Lee JW, Diaz A, Davidson EJ. Efficacy of probiotic use in acute diarrhea in children: a meta-analysis. Dig Dis Sci. 2002;47(11):2625-34. PMID: 12452406
[22] Guarino A, Canani RB, Spagnuolo MI, Albano F, Di Benedetto L. Oral bacterial therapy reduces the duration of symptoms and of viral excretion in children with mild diarrhea. J Pediatr Gastroenterol Nutr. 1997;25(5):516-9. PMID: 9360205
[23] Jiang M, Zhang F, Wan C, Xiong Y, Shah NP, Wei H, et al. Evaluation of probiotic properties of Lactobacillus
plantarum WLPL04 isolated from human breast milk. J Dairy Sci. 2016;99(3):1736-46. PMID: 26805974
[24] Turroni F, Ventura M, Buttó LF, Duranti S, O’Toole PW, Motherway MOC, et al. Molecular dialogue between the human gut microbiota and the host: a Lactobacillus and Bifidobacterium perspective. Cell Mol Life Sci. 2014;71(2):183-203. PMID: 23516017
[25] Gotteland M, Cires MJ, Carvallo C, Vega N, Ramirez MA, Morales P, et al. Probiotic screening and safety evaluation of Lactobacillus strains from plants, artisanal goat cheese, human stools, and breast milk. J Med Food. 2014;17(4):487-95. PMID: 24433075
[26] De Vries MC, Vaughan EE, Kleerebezem M, de Vos WM. Lactobacillus plantarum—survival, functional and potential probiotic properties in the human intestinal tract. Int Dairy J. 2006;16(9):1018-28.
[27] West P, Hewitt J, Murphy OM. The influence of methods of collection and storage on the bacteriology of human milk. J Appl Bacteriol. 1979;46(2):269-77. PMID: 572360
[28] Makino H, Kushiro A, Ishikawa E, Muylaert D, Kubota H, Sakai T, et al. Transmission of intestinal Bifidobacterium longum subsp. longum strains from mother to infant determined by multilocus sequencing typing and amplified fragment length polymorphism. Appl Environ Microbiol. 2011; doi:10.1128. PMID: 21821739
[29] Hugenschmidt S, Schwenninger SM, Gnehm N, Lacroix C. Screening of a natural biodiversity of lactic and propionic acid bacteria for folate and vitamin B12 production in supplemented whey permeate. Int Dairy J. 2010;20(12):852-7.
[30] Paolillo R, Carratelli CR, Sorrentino S, Mazzola N, Rizzo A. Immunomodulatory effects of Lactobacillus plantarum on human colon cancer cells. Int Immunopharmacol. 2009;9(11):1265-71. PMID: 19647100
[31] Yen D, Cheung J, Scheerens H, Poulet F, McClanahan T, Mckenzie B, et al. IL-23 is essential for T cellmediated colitis and promotes inflammation via IL-17 and IL-6. J Clin Invest. 2006;116(5):1310-6. PMID:16670770
[32] Naruszewicz M, Johansson ML, Zapolska-Downar D, Bukowska H. Effect of Lactobacillus plantarum
299v on cardiovascular disease risk factors in smokers. Am J Clin Nutr. 2002;76(6):1249-55. PMID: 12450890
[33] Paineau D, Carcano D, Leyer G, Darquy S, Alyanakian MA, Simoneau G, et al. Effects of seven potential probiotic strains on specific immune responses in healthy adults: a double-blind, randomized, controlled trial. FEMS Immunol Med Microbiol. 2008;53(1):107-13. PMID: 18422632
[34] Daniel C, Poiret S, Goudercourt D, Dennin V, Leyer G, Pot B. Selecting lactic acid bacteria for their safety and functionality by use of a mouse colitis model. Appl Environ Microbiol. 2006;72(9):5799-805. PMID: 16957197
[35] K