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Mahsa Poormoosavi, S., Amin Behmanesh, M., Najafzadeh Varzi, H., Mansouri, S., & Janati, S. (2021). The effect of follicular fluid selenium concentration on oocyte maturation in women with polycystic ovary syndrome undergoing in vitro fertilization/Intracytoplasmic sperm injection: A cross-sectional study. International Journal of Reproductive BioMedicine (IJRM), 19(8), 689-698. https://doi.org/10.18502/ijrm.v19i8.9616
https://doi.org/10.18502/ijrm.v19i8.9616
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- Cited by 5 articles
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Seyedeh Mahsa Poormoosavi
Seyedeh Mahsa Poormoosavi
Department of Histology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran.
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Mohammad Amin Behmanesh
Mohammad Amin Behmanesh
Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
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Hossein Najafzadeh Varzi
Hossein Najafzadeh Varzi
Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
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Shabnam Mansouri
Shabnam Mansouri
School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
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Sima Janati
Sima Janati
Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran.
Published Date
- Sep 9, 2021
The effect of follicular fluid selenium concentration on oocyte maturation in women with polycystic ovary syndrome undergoing in vitro fertilization/Intracytoplasmic sperm injection: A cross-sectional study
Abstract
Background: A high level of free radicals and oxidative substances in women with polycystic ovary syndrome (PCOS) can affect the ovaries through oxidative stress. Antioxidants such as selenium, a vital trace element in human health, can improve the prognosis of PCOS by reducing oxidative stress.
Objective: This study was performed due to the lack of comprehensive information about selenium concentration in follicular fluid and its effect on the oocyte count and quality in infertile women with PCOS.
Materials and Methods: In this cross-sectional study, 78 women with PCOS referred to Umm-al-Banin Infertility Clinic Center, Ganjavian Hospital, Dezful, Iran for in-vitro fertilization from March to November 2019 were enrolled. After ovarian stimulation with the antagonist protocol, the oocytes were retrieved under transvaginal ultrasound in in-vitro fertilization/intracytoplasmic sperm injection cycles, and selenium concentrations were measured in the follicular fluid using an atomic absorption method by spectrophotometer device. Oocyte count and morphology were evaluated using inverted optical microscopy.
Results: There were no significant differences between follicular fluid selenium concentrations in terms of the total number of oocytes and immature oocytes in the metaphase I and germinal vesicle stages. However, a significantly reduced number of metaphase II oocytes was observed at selenium levels < 40 μg/dL (p = 0.001).
Conclusion: Based on our results, low levels of follicular selenium concentration in infertile women with PCOS can reduce the quality and potency of oocyte maturation.
Key words: Polycystic ovary syndrome, Oxidative stress, Selenium, In vitro fertilization, Oocyte quality, Follicular fluid.
References
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[2] Eslamian Gh, Hekmatdoost A. Nutrient patterns and risk of polycystic ovary syndrome. J Reprod Infertil 2019; 20: 161–168.
[3] Zhang Y, Liu L, Yin TL, Yang J, Xiong ChL. Follicular metabolic changes and effects on oocyte quality in polycystic ovary syndrome patients. Oncotarget 2017; 8: 80472–80480.
[4] Papalou O, Victor MV, Diamanti-Kandarakis E. Oxidative stress in polycystic ovary syndrome. Current Pharmaceutical Design 2016; 22: 2709– 2722.
[5] Fabjan T, Vrtačnik-Bokal E, Kumer K, Osredkar J. Determination of oxidative stress balance in follicular fluid. J Lab Med 2018; 42: 51–58.
[6] Poormoosavi SM, Behmanesh MA, Janati S, Najafzadehvarzi H. Level of bisphenol a in follicular fluid and serum and oocyte morphology in patients undergoing IVF treatment. J Family Reprod Health 2019; 13: 154–159.
[7] Chakraborty P, Ghosh S, Goswami SK, Kabir SN, Chakravarty B, Jana K. Altered trace mineral milieu might play an aetiological role in the pathogenesis of polycystic ovary syndrome. Biol Trace Elem Res 2013; 152: 9–15.
[8] Singh AK, Chattopadhyay R, Chakravarty B, Chaudhury K. Markers of oxidative stress in follicular fluid of women with endometriosis and tubal infertility undergoing IVF. Reprod Toxicol 2013; 42: 116–124.
[9] Kim YS, Kim MS, Lee SH, Choi BCh, Lim JM, Cha KY, et al. Proteomic analysis of recurrent spontaneous abortion: Identification of an inadequately expressed set of proteins in human follicular fluid. Proteomics 2006; 6: 3445–3454.
[10] Turan V, Sezer ED, Zeybek B, Sendag F. Infertility and the presence of insulin resistance are associated with increased oxidative stress in young, non-obese turkish women with polycystic ovary syndrome. J Pediatr Adolesc Gynecol 2015; 28: 119–123.
[11] Coskun A, Arikan T, Kilinc M, Arikan DC, Ekerbiçer HÇ. Plasma selenium levels in Turkish women with polycystic ovary syndrome. Eur J Obstet Gynecol Reprod Biol 2013; 168: 183–186.
[12] Razavi M, Jamilian M, Fakhrieh Kashan Z, Heidar Z, Mohseni M, Ghandi Y, et al. Selenium supplementation and the effects on reproductive outcomes, biomarkers of inflammation, and oxidative stress in women with polycystic ovary syndrome. Horm Metab Res 2016; 48: 185–190.
[13] Kipp AP. Selenium and selenoproteins in redox signaling. Free Radical Biology and Medicine 2017; 108 (Suppl.): S1–S3.
[14] Duntas LH, Hubalewska-Dydejczyk A. Selenium and Inflammation-potential use and future perspectives. US Endocrinol 2015; 11: 97–102.
[15] Lim AS, Tsakok MF. Age-related decline in fertility: A link to degenerative oocytes? Fertil Steril 1997; 68: 265–271.
[16] Jukola E, Hakkarainen J, Saloniemi H, Sankari S. Blood selenium, vitamin E, vitamin A, and β-carotene concentrations and udder health, fertility treatments, and fertility. J Dairy Sci 1996; 79: 838–845.
[17] Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril 2004; 81: 19–25.
[18] Scott L, Alvero R, Leondires M, Miller B. The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. Hum Reprod 2000; 15: 2394–2403.
[19] Bahadori MH, Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF. Level of vitamin e in follicular fluid and serum and oocyte morphology and embryo quality in patients undergoing ivf treatment. J Family Reprod Health 2017; 11: 74–81.
[20] Čuparigova F, Stafilov T. Determination of selenium in human blood serum by electrothermal atomic absorption spectrometry. Chem Sci J 2011; 46: CSJ- 46. 1–8.
[21] Coticchio G, Dal Canto M, Mignini Renzini M, Guglielmo MC, Brambillasca F, Turchi D, et al. Oocyte maturation: Gamete-somatic cells interactions, meiotic resumption, cytoskeletal dynamics and cytoplasmic reorganization. Hum Reprod Update 2015; 21: 427–454.
[22] Da Broi MG, Giorgi VSI, Wang F, Keefe DL, Albertini D, Navarro PA. Influence of follicular fluid and cumulus cells on oocyte quality: Clinical implications. J Assist Reprod Genet 2018; 35: 735–751.
[23] Hennet ML, Combelles CMH. The antral follicle: A microenvironment for oocyte differentiation. Int J Dev Biol 2012; 56: 819–831.
[24] Shkolnik K, Tadmor A, Ben-Dor Sh, Nevo N, Galiani D, Dekel N. Reactive oxygen species are indispensable in ovulation. Proc Natl Acad Sci USA 2011; 108: 1462–1467.
[25] Choi WJ, Banerjee J, Falcone T, Bena J, Agarwal A, Sharma RK. Oxidative stress and tumor necrosis factor-α-induced alterations in metaphase II mouse oocyte spindle structure. Fertil Steril 2007; 88: 1220–1231.
[26] Maedomari N, Kikuchi K, Ozawa M, Noguchi J, Kaneko H, Ohnuma K, et al. Cytoplasmic glutathione regulated by cumulus cells during porcine oocyte maturation affects fertilization and embryonic development in vitro. Theriogenology 2007; 67: 983–993.
[27] Ceko MJ, Hummitzsch K, Hatzirodos N, Bonner WM, Aitken JB, Russell DL, et al. X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function. Metallomics 2014; 7: 188.
[28] Özkaya MO, Naziroğlu M, Barak C, Berkkanoglu M. Effects of multivitamin/mineral supplementation on trace element levels in serum and follicular fluid of women undergoing in vitro fertilization (IVF). Biol Trace Elem Res 2011; 139: 1–9.
[29] Xiong X, Lan D, Li J, Lin Y, Li M. Selenium supplementation during in vitro maturation enhances meiosis and developmental capacity of yak oocytes. Anim Sci J 2018; 89: 298–306.
[30] Qiao J, Feng HL. Extra-and intra-ovarian factors in polycystic ovary syndrome: Impact on oocyte maturation and embryo developmental competence. Hum Reprod Update 2011; 17: 17–33.
[31] Cimadomo D, Fabozzi G, Vaiarelli A, Ubaldi N, Ubaldi FM, Rienzi L. Impact of maternal age on oocyte and embryo competence. Front Endocrinol 2018; 9: 327.
[32] Volarcik K, Sheean L, Goldfarb J, Woods L, Abdul- Karim FW, Hunt P. The meiotic competence of invitro matured human oocytes is influenced by donor age: Evidence that folliculogenesis is compromised in the reproductively aged ovary. Hum Reprod 1998; 13: 154–160.
[33] Smits MAJ, Wong KM, Mantikou E, Korver CM, Jongejan A, Breit TM, et al. Age-related gene expression profiles of immature human oocytes. Mol Hum Reprod 2018; 24: 469–477.
[34] Gonca S, Gün I, Ovayolu A, Şilfeler D, Sofuoğlu K, Özdamar Ö, et al. Effect of lower than expected number of oocyte on the IVF results after oocytepickup. Int J Clin Exp Med 2014; 7: 1853–1859.
[35] Bu Zh, Dai W, Guo Y, Su Y, Zhai J, Sun Y. Overweight and obesity adversely affect outcomes of assisted reproductive technologies in polycystic ovary syndrome patients. Int J Clin Exp Med 2013; 6: 991–995.
[2] Eslamian Gh, Hekmatdoost A. Nutrient patterns and risk of polycystic ovary syndrome. J Reprod Infertil 2019; 20: 161–168.
[3] Zhang Y, Liu L, Yin TL, Yang J, Xiong ChL. Follicular metabolic changes and effects on oocyte quality in polycystic ovary syndrome patients. Oncotarget 2017; 8: 80472–80480.
[4] Papalou O, Victor MV, Diamanti-Kandarakis E. Oxidative stress in polycystic ovary syndrome. Current Pharmaceutical Design 2016; 22: 2709– 2722.
[5] Fabjan T, Vrtačnik-Bokal E, Kumer K, Osredkar J. Determination of oxidative stress balance in follicular fluid. J Lab Med 2018; 42: 51–58.
[6] Poormoosavi SM, Behmanesh MA, Janati S, Najafzadehvarzi H. Level of bisphenol a in follicular fluid and serum and oocyte morphology in patients undergoing IVF treatment. J Family Reprod Health 2019; 13: 154–159.
[7] Chakraborty P, Ghosh S, Goswami SK, Kabir SN, Chakravarty B, Jana K. Altered trace mineral milieu might play an aetiological role in the pathogenesis of polycystic ovary syndrome. Biol Trace Elem Res 2013; 152: 9–15.
[8] Singh AK, Chattopadhyay R, Chakravarty B, Chaudhury K. Markers of oxidative stress in follicular fluid of women with endometriosis and tubal infertility undergoing IVF. Reprod Toxicol 2013; 42: 116–124.
[9] Kim YS, Kim MS, Lee SH, Choi BCh, Lim JM, Cha KY, et al. Proteomic analysis of recurrent spontaneous abortion: Identification of an inadequately expressed set of proteins in human follicular fluid. Proteomics 2006; 6: 3445–3454.
[10] Turan V, Sezer ED, Zeybek B, Sendag F. Infertility and the presence of insulin resistance are associated with increased oxidative stress in young, non-obese turkish women with polycystic ovary syndrome. J Pediatr Adolesc Gynecol 2015; 28: 119–123.
[11] Coskun A, Arikan T, Kilinc M, Arikan DC, Ekerbiçer HÇ. Plasma selenium levels in Turkish women with polycystic ovary syndrome. Eur J Obstet Gynecol Reprod Biol 2013; 168: 183–186.
[12] Razavi M, Jamilian M, Fakhrieh Kashan Z, Heidar Z, Mohseni M, Ghandi Y, et al. Selenium supplementation and the effects on reproductive outcomes, biomarkers of inflammation, and oxidative stress in women with polycystic ovary syndrome. Horm Metab Res 2016; 48: 185–190.
[13] Kipp AP. Selenium and selenoproteins in redox signaling. Free Radical Biology and Medicine 2017; 108 (Suppl.): S1–S3.
[14] Duntas LH, Hubalewska-Dydejczyk A. Selenium and Inflammation-potential use and future perspectives. US Endocrinol 2015; 11: 97–102.
[15] Lim AS, Tsakok MF. Age-related decline in fertility: A link to degenerative oocytes? Fertil Steril 1997; 68: 265–271.
[16] Jukola E, Hakkarainen J, Saloniemi H, Sankari S. Blood selenium, vitamin E, vitamin A, and β-carotene concentrations and udder health, fertility treatments, and fertility. J Dairy Sci 1996; 79: 838–845.
[17] Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril 2004; 81: 19–25.
[18] Scott L, Alvero R, Leondires M, Miller B. The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. Hum Reprod 2000; 15: 2394–2403.
[19] Bahadori MH, Sharami SH, Fakor F, Milani F, Pourmarzi D, Dalil-Heirati SF. Level of vitamin e in follicular fluid and serum and oocyte morphology and embryo quality in patients undergoing ivf treatment. J Family Reprod Health 2017; 11: 74–81.
[20] Čuparigova F, Stafilov T. Determination of selenium in human blood serum by electrothermal atomic absorption spectrometry. Chem Sci J 2011; 46: CSJ- 46. 1–8.
[21] Coticchio G, Dal Canto M, Mignini Renzini M, Guglielmo MC, Brambillasca F, Turchi D, et al. Oocyte maturation: Gamete-somatic cells interactions, meiotic resumption, cytoskeletal dynamics and cytoplasmic reorganization. Hum Reprod Update 2015; 21: 427–454.
[22] Da Broi MG, Giorgi VSI, Wang F, Keefe DL, Albertini D, Navarro PA. Influence of follicular fluid and cumulus cells on oocyte quality: Clinical implications. J Assist Reprod Genet 2018; 35: 735–751.
[23] Hennet ML, Combelles CMH. The antral follicle: A microenvironment for oocyte differentiation. Int J Dev Biol 2012; 56: 819–831.
[24] Shkolnik K, Tadmor A, Ben-Dor Sh, Nevo N, Galiani D, Dekel N. Reactive oxygen species are indispensable in ovulation. Proc Natl Acad Sci USA 2011; 108: 1462–1467.
[25] Choi WJ, Banerjee J, Falcone T, Bena J, Agarwal A, Sharma RK. Oxidative stress and tumor necrosis factor-α-induced alterations in metaphase II mouse oocyte spindle structure. Fertil Steril 2007; 88: 1220–1231.
[26] Maedomari N, Kikuchi K, Ozawa M, Noguchi J, Kaneko H, Ohnuma K, et al. Cytoplasmic glutathione regulated by cumulus cells during porcine oocyte maturation affects fertilization and embryonic development in vitro. Theriogenology 2007; 67: 983–993.
[27] Ceko MJ, Hummitzsch K, Hatzirodos N, Bonner WM, Aitken JB, Russell DL, et al. X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function. Metallomics 2014; 7: 188.
[28] Özkaya MO, Naziroğlu M, Barak C, Berkkanoglu M. Effects of multivitamin/mineral supplementation on trace element levels in serum and follicular fluid of women undergoing in vitro fertilization (IVF). Biol Trace Elem Res 2011; 139: 1–9.
[29] Xiong X, Lan D, Li J, Lin Y, Li M. Selenium supplementation during in vitro maturation enhances meiosis and developmental capacity of yak oocytes. Anim Sci J 2018; 89: 298–306.
[30] Qiao J, Feng HL. Extra-and intra-ovarian factors in polycystic ovary syndrome: Impact on oocyte maturation and embryo developmental competence. Hum Reprod Update 2011; 17: 17–33.
[31] Cimadomo D, Fabozzi G, Vaiarelli A, Ubaldi N, Ubaldi FM, Rienzi L. Impact of maternal age on oocyte and embryo competence. Front Endocrinol 2018; 9: 327.
[32] Volarcik K, Sheean L, Goldfarb J, Woods L, Abdul- Karim FW, Hunt P. The meiotic competence of invitro matured human oocytes is influenced by donor age: Evidence that folliculogenesis is compromised in the reproductively aged ovary. Hum Reprod 1998; 13: 154–160.
[33] Smits MAJ, Wong KM, Mantikou E, Korver CM, Jongejan A, Breit TM, et al. Age-related gene expression profiles of immature human oocytes. Mol Hum Reprod 2018; 24: 469–477.
[34] Gonca S, Gün I, Ovayolu A, Şilfeler D, Sofuoğlu K, Özdamar Ö, et al. Effect of lower than expected number of oocyte on the IVF results after oocytepickup. Int J Clin Exp Med 2014; 7: 1853–1859.
[35] Bu Zh, Dai W, Guo Y, Su Y, Zhai J, Sun Y. Overweight and obesity adversely affect outcomes of assisted reproductive technologies in polycystic ovary syndrome patients. Int J Clin Exp Med 2013; 6: 991–995.