https://doi.org/10.18502/ijrm.v19i7.9471
statistics
- 196 Abstract Views
- 110 PDF Views
- 0 HTML Views
- Cited by 1 article
authors
-
Fatemeh Rahimi Asl
Fatemeh Rahimi Asl
Department of Biology, Islamic Azad University North Tehran Branch, Tehran, Iran
-
Maryam Khosravi
Maryam Khosravi
Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran
-
Ramin Hajikhani
Ramin Hajikhani
Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran
-
Jalal Solati
Jalal Solati
Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
-
Hossein Fahimi
Hossein Fahimi
Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Published Date
- Aug 16, 2021
Complementary effects of coenzyme Q10 and Lepidium sativum supplementation on the reproductive function of mice: An experimental study
Abstract
Background: Coenzyme Q10 (CoQ10) and Lepidium sativum (LS) have therapeutic effects on infertility.
Objective: To evaluate the combined effects of LS and CoQ10 on reproductive function in adult male NMRI mice.
Materials and Methods: Eighty three-months-old male mice (35–40 gr) were divided into four groups (n = 10/each): control (treated with water), CoQ10-treated (200, 300, and 400 mg/kg/body weight), LS-treated (200, 400, 600 mg/kg/body weight), and co-treated (LS [600 mg/kg/body weight] + CoQ10 [200 mg/kg/body weight]) groups. Serum testosterone, luteinizing hormone, follicle-stimulating hormone, and gonadotropin realizing hormone (GnRH) levels were measured using ELISA method. The sperm quality was assessed using Sperm Class Analyzer® (SCA) CASA system and GnRH mRNA expression levels were evaluated by real-time polymerase chain reaction.
Results: The number of sniffing and following behavior was significantly higher in LStreated (400 and 600 mg/ml/body weight) groups than the control group (p = 0.0007 and p = 0.0010, respectively). The number of mounting and coupling behaviors was significantly higher in the CoQ10 (300 and 400 mg/ml/body weight)-treated animals than the control group (p = 0.0170 and p = 0.0006, respectively). Co-treatment of CoQ10 (200 mg/ml/body weight) and LS (600 mg/ml/body weight) significantly increased all aspects of sexual behaviors as well as the levels of serum testosterone (p = 0.0011), luteinizing hormone (p = 0.0062), and follicle-stimulating hormone (p = 0.0001); sperm viability (p = 0.0300) and motility (p = 0.0010); and GnRH mRNA levels (p = 0.0016) compared to the control group.
Conclusion: The coadministration of CoQ10 and LS significantly improves the activity of the hypothalamic-pituitary-gonadal axis and enhances the reproductive parameters in adult male mice.
Key words: Lepidium sativum, Coenzyme Q10, Infertility, Male reproductive function.
References
[2] Barratt ChLR, Björndahl L, De Jonge ChJ, Lamb DJ, Osorio Martini F, McLachlan R, et al. The diagnosis of male infertility: An analysis of the evidence to support the development of global WHO guidance-challenges and future research opportunities. Hum Reprod Update 2017; 23: 660–680.
[3] Oyeyemi MO, Olukole SG, Esan O. Sperm morphological studies of West African Dwarf Bucks treated with pumpkin plant (Cucurbita pepo). Int J Morphol 2008; 26: 121–126.
[4] Imade OV, Erinfolami WA, Ajadi RA, Abioja MO, Rahman SA, Smith OF, et al. Effects of Lepidium sativum supplementation on growth and gonadotropins secretion in ovariectomized, estrogen-implanted rabbits. Asian Pac J Reprod 2018; 7: 155–160.
[5] Kamani M, Seyed Hosseini E, Haddad Kashani H, Atlasi MA, Nikzad H. Protective effect of Lepidium sativum seed extract on histopathology and morphology of epididymis in the diabetic rat model. Int J Morphol 2017; 35: 603–610.
[6] Gonzales GF, Cordova A, Gonzales C, Chung A, Vega K, Villena A. Lepidium meyenii (Maca) improved semen parameters in adult men. Asian J Androl 2001; 3: 301–303.
[7] Rehman NU, Khan A, Alkharfy KhM, Gilani AH. Pharmacological basis for the medicinal use of Lepidium sativum in airways disorders. J Evidence-Based Complement Altern Med 2012; 2012: 596524.
[8] Patel U, Kulkarni M, Undale V, Bhosale A. Evaluation of diuretic activity of aqueous and methanol extracts of Lepidium sativum garden cress (Cruciferae) in rats. Trop J Pharm Res 2009; 8: 215–219.
[9] Gonzales GF, Ruiz A, Gonzales C, Villegas L, Cordova A. Effect of Lepidium meyenii (maca) roots on spermatogenesis of male rats. Asian J Androl 2001; 3: 231–233.
[10] Abdallah HM, Farag MA, Algandaby MM, Nasrullah MZ, Abdel-Naim AB, Eid BG, et al. Osteoprotective activity and metabolite fingerprint via UPLC/MS and GC/MS of Lepidium sativum in ovariectomized rats. Nutrients 2020; 12: 2075. 1–20.
[11] Uchiyama F, Jikyo T, Takeda R, Ogata M. Lepidium meyenii (Maca) enhances the serum levels of luteinising hormone in female rats. J Ethnopharmacol 2014; 151: 897–902.
[12] Aoki Y, Tsujimura A, Nagashima Y, Hiramatsu I, Uesaka Y, Nozaki T, et al. Effect of Lepidium meyenii on in vitro fertilization via improvement in acrosome reaction and motility of mouse and human sperm. Reprod Med Biol 2018; 18: 57–64.
[13] Duru NK, Morshedi M, Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil Steril 2000; 74: 1200–1207.
[14] Henkel RR. Leukocytes and oxidative stress: Dilemma for sperm function and male fertility. Asian J Androl 2011; 13: 43–52.
[15] Asgari S, Ansari Samani R, Deris F, Shahinfard N, Salimi M, Mortazaei S, et al. Antioxidant activity and the lowering effect of hydroalcoholic extract of Allium hirtifolium boisson some haemostatic factors in hypercholesterolemic rabbits. J Mazandaran Univ Med Sci 2012; 22: 40–48.
[16] Lafuente R, González-Comadrán M, Solà I, López G, Brassesco M, Carreras R, et al. Coenzyme Q10 and male infertility: A meta-analysis. J Assist Reprod Genet 2013; 30: 1147–1156.
[17] Eddouks M, Maghrani M, Zeggwagh NA, Michel J. Study of the hypoglycaemic activity of Lepidium sativum L. aqueous extract in normal and diabetic rats. J Ethnopharmacol 2005; 97: 391–395.
[18] Victoria IO, Fedrick SO, Oladele G, Oluwafemi AE, Atiang BJ. Effects of Lepidium sativum seed on reproductive characteristics in rabbit bucks. J Phytopharmacol 2020; 9: 89–95.
[19] Abdulidha NA, Jaccob AA, AL-Moziel MS. Protective effects of Co-Q10, Ginkgo biloba, and l-carnitine on brain, kidney, liver, and endocrine system against sub-acute heavy metals toxicity in male rats. Toxicol Environ Health Sci 2020; 12: 331–341.
[20] Working PK. Male reproductive toxicology: Comparison of the human to animal models. Environmental Health Perspectives 1988; 77: 37–44.
[21] Stachecki JJ, Ginsburg KA, Leach RE, Armant DR. Computer-assisted semen analysis (CASA) of epididymal sperm from the domestic cat. Asian J Androl 1993; 14: 60–65.
[22] Jovanović Z. [Oxidative stress in the pathogenesis of neurodegenerative diseases]. Med Čas 2011; 45: 16–23. (in Bosnian)
[23] Aly NH. Reno-protective efficiency of coenzyme Q10 on adriamycin-induced nephrotoxicity in rats. J Appl Sci Res 2012; 8: 589–597.
[24] Palmeira CM, Santos DL, Seiça R, Moreno AJ, Santos MS. Enhanced mitochondrial testicular antioxidant capacity in Goto-Kakizaki diabetic rats: Role of coenzyme Q. Am J Physiol Cell Physiol 2001; 281: C1023–C1028.
[25] Ghanbarzadeh S, Garjani A, Ziaee M, Khorrami A. Effects of L-carnitine and coenzyme q10 on impaired spermatogenesis caused by isoproterenol in male rats. Drug Res 2014; 64: 449–453.
[26] Mali RG, Mahajan SG, Mehta AA. Lepidium sativum (Garden cress): A review of contemporary literature and medicinal properties. Oriental Pharm Exp Med 2007; 7: 331–335.
[27] Chauhan NS, Sharma V, Dixit VK, Thakur M. A review on plants used for improvement of sexual performance and virility. BioMed Res Int 2014; 2014: 868062. 1–20.
[28] Gonzales GF. Ethnobiology and ethnopharmacology of Lepidium meyenii (Maca), a plant from the Peruvian highlands. Evid Based Complement Altern Med 2012; 2012: 193496. 1–10.
[29] Tsigos C, Chrousos GP. Hypothalamic-pituitaryadrenal axis, neuroendocrine factors and stress. J Psychosom Res 2002; 53: 865–871.
[30] Banihani SA. Effect of coenzyme q10 supplementation on testosterone. Biomolecules 2018; 8: 172. 1–9.
[31] Tiseo BC, Gaskins AJ, Hauser R, Chavarro JE, Tanrikut C, Team ES. Coenzyme Q10 intake from food and semen parameters in a subfertile population. Urology 2017; 102: 100–105.
[32] Xu Y, Nisenblat V, Lu C, Li R, Qiao J, Zhen X, et al. Pretreatment with coenzyme Q10 improves ovarian response and embryo quality in lowprognosis young women with decreased ovarian reserve: A randomized controlled trial. Reprod Biol Endocrinol 2018; 16: 29. 1–11.