https://doi.org/10.18502/ijrm.v19i5.9254
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- Cited by 8 articles
authors
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Kaivalya Gongadashetti
Kaivalya Gongadashetti
Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India
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Pankush Gupta
Pankush Gupta
Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India
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Rima Dada
Rima Dada
Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India
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Neena Mahotra
Neena Mahotra
Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India
Published Date
- Jun 23, 2021
Follicular fluid oxidative stress biomarkers and ART outcomes in PCOS women undergoing in vitro fertilization: A cross-sectional study
Abstract
Background: Polycystic ovarian syndrome (PCOS) is the most common cause of anovulatory infertility. Oxidative stress (OS), which plays an important role in determining the developmental competence of an oocyte, may be involved in understanding infertility and poor outcomes cycles in PCOS women undergoing in vitro fertilization (IVF).
Objective: To measure OS biomarkers in the follicular fluid of PCOS women undergoing IVF.
Materials and Methods: In this cross-sectional study, 100 women with PCOS (n = 43) and tubal factor (n = 57) undergoing IVF, who were referred to a tertiary medical center between January 2016 and September 2017 were enrolled. OS markers like reactive oxygen species (ROS), total antioxidant capacity (TAC), and 8-Isoprostane (8-IP) were tested in the follicular fluid and various IVF outcomes in the form of oocytes retrieved, fertilized, cleavage rate, grading of embryos and pregnancy outcomes were compared between the two groups.
Results: The results indicated that the levels of ROS, TAC, and 8-IP were higher in the PCOS group compared to the tubal group (p = 0.21, p = 0.95, and p < 0.05, respectively). Biomarkers based on the number of retrieved oocytes, cleavage rate, and grades of embryos did not differ significantly in the two groups. The median ROS, TAC, and 8-IP were not significantly different in the two groups in relation to the pregnancies, although the 8-IP levels were significantly raised in the PCOS women who had a miscarriage (p = 0.02).
Conclusion: This study concluded the possible role of OS in PCOS women with increased higher level of 8-IP biomarker as a potential biomarker that needs further evaluation.
Key words: Oxidative stress, ART, PCOS, Infertility, 8-IP.
References
[2] Victor VM, Rocha M, Bañuls C, Alvarez A, Pablo CD, Sanchez-Serrano M, et al. Induction of oxidative stress and human leukocyte/endothelial cell interactions in polycystic ovary syndrome patients with insulin resistance. J Clin Endocrinol Metab 2011; 96: 3115–3122.
[3] Yeon Lee J, Baw CK, Gupta S, Aziz N, Agarwal A. Role of oxidative stress in polycystic ovary Syndrome. Curr Womens Health Rev 2010; 6: 96–107.
[4] Da Broi MG, Giorgi VSI, Wang F, Keefe DL, Albertini D, Navarro PA. Influence of follicular fluid and cumulus cells on oocyte quality: Clinical implications. J Assist Reprod Genet 2018; 35: 735–751.
[5] Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3: 1–21.
[6] Dinger Y, Akcay T, Erdem T, Ilker Saygili E, Gundogdu S. DNA damage, DNA susceptibility to oxidation and glutathione level in women with polycystic ovary syndrome. Scand J Clin Lab Invest 2005; 65: 721–728.
[7] Botros L, Sakkas D, Seli E. Metabolomics and its application for non-invasive embryo assessment in IVF. Mol Hum Reprod 2008; 14: 679–690.
[8] Das S, Chattopadhyay R, Ghosh S, Ghosh S, Goswami SK, Chakravarty BN, et al. Reactive oxygen species level in follicular fluid-embryo quality marker in IVF? Hum Reprod 2006; 21: 2403–2407.
[9] Attaran M, Pasqualotto E, Falcone T, Goldberg JM, Miller KF, Agarwal A, et al. The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int J Fertil Womens Med 2000; 45: 314–320.
[10] Bedaiwy MA, Falcone T, Mohamed MS, Aleem AAN. Differential growth of human embryos in vitro: Role of reactive oxygen species. Fertil Steril 2004; 82: 593–600.
[11] Sabatini L, Wilson C, Lower A, Al-Shawaf T, Grudzinskas JG. Superoxide dismutase activity in human follicular fluid after controlled ovarian hyperstimulation in women undergoing in vitro fertilization. Fertil Steril 1999; 72: 1027–1034.
[12] Oyawoye O, Abdel Gadir A, Garner A, Constantinovici N, Perrett C, Hardiman P. Antioxidants and reactive oxygen species in follicular fluid of women undergoing IVF: Relationship to outcome. Hum Reprod 2003; 18: 2270–2274.
[13] Bedaiwy M, Agarwal A, Said TM, Goldberg JM, Sharma RK, Worley S, et al. Role of total antioxidant capacity in the differential growth of human embryos in vitro. Fertil Steril 2006; 86: 304–309.
[14] Pasqualotto EB, Agarwal A, Sharma RK, Izzo VM, Pinotti JA, Joshi NJ, et al. Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures. Fertil Steril 2004; 81: 973–976.
[15] Khosrowbeygi A, Zarghami N. Levels of oxidative stress biomarkers in seminal plasma and their relationship with seminal parameters. BMC Clin Pathol 2007; 7: 1–6.
[16] Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril 2004; 81: 19–25.
[17] Yadav V, Bakolia P, Malhotra N, Mahey R, Singh N, Kriplani A. Comparison of obstetric outcomes of pregnancies after donor-oocyte In vitro fertilization and self-oocyte In vitro fertilization: A retrospective cohort study. J Hum Reprod Sci 2018; 11: 370–375.
[18] Malhotra N, Srivastava A, Rana H, Bahadur A, Sengupta J, Ghosh D. Comparative multiplex analysis of cytokines, chemokines and growth factors in follicular fluid of normoresponder women undergoing ovum donation with gonadotropin-releasing hormone agonist versus gonadotropin-releasing hormone antagonist protocols. J Hum Reprod Sci 2013; 6: 205–212.
[19] ALPHA Scientists In Reproductive Medicine, ESHRE Special Interest Group Embryology. Istanbul consensus workshop on embryo assessment: Proceedings of an expert meeting. Reprod Biomed Online 2011; 22: 632–646.
[20] Jozwik M, Wolczynski S, Jozwik M, Szamatowicz M. Oxidative stress markers in preovulatory follicular fluid in humans. Mol Hum Reprod 1999; 5: 409–413.
[21] Younis A, Clower C, Nelsen D, Butler W, Carvalho A, Hok E, et al. The relationship between pregnancy and oxidative stress markers on patients undergoing ovarian stimulations. J Assist Reprod Genet 2012; 29: 1083–1089.
[22] Halliwell B, Lee CYJ. Using isoprostanes as biomarkers of oxidative stress: Some rarely considered issues. Antioxid Redox Signal 2010; 13: 145–156.
[23] Meagher EA, Fitz Gerald GA. Indices of lipid peroxidation in vivo: Strengths and limitations. Free Radic Biol Med 2000; 28: 1745–1750.
[24] Lin K, Barnhart K, Shaunik A, Butts S, Fitzgerald GA, Coutifaris C. Follicular fluid F2-isoprostanes: A novel assessment of oxidative stress in IVF patients. Fertil Steril 2005; 84: S47.
[25] Ruder EH, Hartman TJ, Blumberg J, Goldman MB. Oxidative stress and antioxidants: Exposure and impact on female fertility. Hum Reprod Update 2008; 14: 345–357.
[26] Aseervatham GSB, Sivasudha T, Jeyadevi R, Arul Ananth D. Environmental factors and unhealthy lifestyle influence oxidative stress in humans--An overview. Environ Sci Pollut Res Int 2013; 20: 4356–4369.
[27] Sies H, Stahl W, Sevanian A. Nutritional, dietary and postprandial oxidative stress. J Nutr 2005; 135: 969–672.