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Prasad Mary, A., Nandeesha, H., Papa, D., Chitra, T., Nachiappa Ganesh, R., & Menon, V. (2021). Matrix metalloproteinase-9 is elevated and related to interleukin-17 and psychological stress in male infertility: A cross-sectional study. International Journal of Reproductive BioMedicine (IJRM), 19(4), 333–338. https://doi.org/10.18502/ijrm.v19i4.9059
https://doi.org/10.18502/ijrm.v19i4.9059
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authors
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Ann Prasad Mary
Ann Prasad Mary
Biochemistry Department, Jawaharlal Institute of Postgraduate Medical Education and Research ( JIPMER), Puducherry, India
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Hanumanthappa Nandeesha
Hanumanthappa Nandeesha
Biochemistry Department, Jawaharlal Institute of Postgraduate Medical Education and Research ( JIPMER), Puducherry, India
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Dasari Papa
Dasari Papa
Department of Obstetrics and Gynecology, Jawaharlal Institute of Postgraduate Medical Education and Research ( JIPMER), Puducherry, India
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Thyagaraju Chitra
Thyagaraju Chitra
Department of Obstetrics and Gynecology, Jawaharlal Institute of Postgraduate Medical Education and Research ( JIPMER), Puducherry, India
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Rajesh Nachiappa Ganesh
Rajesh Nachiappa Ganesh
Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research ( JIPMER), Puducherry, India
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Vikas Menon
Vikas Menon
Department of Psychiatry, Jawaharlal Institute of Postgraduate Medical Education and Research ( JIPMER), Puducherry, India
Published Date
- Apr 22, 2021
Matrix metalloproteinase-9 is elevated and related to interleukin-17 and psychological stress in male infertility: A cross-sectional study
Abstract
Background: Matrix metalloproteinase-9 (MMP-9), interleukin-17 (IL-17) and psychological stress are known to play a role in the pathogenesis of male infertility.
Objective: To assess the association of MMP-9 with IL-17 and psychological stress in infertile men. Materials and Methods: In this cross-sectional study, 39 men with infertility diagnosed based on semen analysis and 39 subjects with normal semen analysis were included in the study. MMP-9 and IL-17 were estimated in both groups by ELISA. Perceived stress scale was used to assess psychological stress in controls and cases.
Results: In infertile cases, MMP-9 and IL-17 were significantly increased when compared with controls (p = 0.046, p = 0.041 respectively). A significant association of MMP-9 was observed with IL-17 (r = 0.335, p = 0.037) and perceived stress scale (r = 0.329, p = 0.041).
Conclusion: IL-17 and stress increase MMP- 9 levels in infertile men.
Key words: Infertility, Interleukins, Peptide hydrolase.
References
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[2] Mishra P, Negi MPS, Srivastava M, Singh K, Rajender S. Decline in seminal quality in Indian men over the last 37 years. Reprod Biol Endocrinol 2018; 16: 103–104.
[3] Duan YG, Yu CF, Novak N, Bieber T, Zhu CH, Schuppe HC, et al. Immunodeviation towards a Th17 immune response associated with testicular damage in azoospermic men. Int J Androl 34: e536–e545.
[4] Qian LN, Shi Q, Gu Y, Song J, Zhou M, Hua M. The relationship between IL-17 and male infertility: Semen analysis. African J Microbiol Res 2012; 6: 5672–5677.
[5] Belardin LB, Antoniassi MP, Camargo M, Intasqui P, Fraietta R, Bertolla RP. Semen levels of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinases (TIMP) protein families members in men with high and low sperm DNA fragmentation. Sci Rep 2019; 9: 903–904.
[6] Szarek M, Bergmann M, Konrad L, Schuppe HC, Kliesch S, Hedger MP, et al. Activin A target genes are differentially expressed between normal and neoplastic adult human testes: Clues to gonocyte fate choice. Andrology 2019; 7: 31–41.
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[8] Nargund VH. Effects of psychological stress on male fertility. Nat Rev Urol 2015; 12: 373–382.
[9] Wirleitner B, Vanderzwalmen P, Stecher A, Spitzer D, Schuff M, Schwerda D, et al. Dietary supplementation of antioxidants improves semen quality of IVF patients in terms of motility, sperm count, and nuclear vacuolization. Int J Vitam Nutr Res 2012; 82: 391–398.
[10] Murray KS, James A, McGeady JB, Reed ML, Kuang WW, Nangia AK. The effect of the new 2010 world health organization criteria for semen analyses on male infertility. Fertil Steril 2012; 98: 1428–1431.
[11] Tentes I, Asimakopoulos B, Mourvati E, Diedrich K, Al- Hasani S, Nikolettos N. Matrix metalloproteinase (MMP)- 2 and MMP-9 in seminal plasma. J Assist Reprod Genet 2007; 24: 278–281.
[12] Nielsen MG, Ørnbøl E, Vestergaard M, Bech P, Larsen FB, Lasgaard M, et al. The construct validity of the perceived stress scale. J Psychosom Res 2016; 84: 22–30.
[13] Agarwal A, Rana M, Qiu E, Al Bunni H, Bui AD, Henkel R. Role of oxidative stress, infection and inflammation in male infertility. Andrologia 2018; 50: e13126.
[14] Sabbaghi M, Aram R, Roustaei H, Fadavi Islam M, Daneshvar M, Castaño AR, et al. IL-17A concentration of seminal plasma and follicular fluid in infertile men and women with various clinical diagnoses. Immunol Invest 2014; 43: 617–626.
[15] Lotti F, Tamburrino L, Marchiani S, Maseroli E, Vitale P, Forti G, et al. DNA fragmentation in two cytometric sperm populations: Relationship with clinical and ultrasound characteristics of the male genital tract. Asian J Androl 2017; 19: 272–279.
[16] Ilacqua A, Izzo G, Emerenziani GP, Baldari C, Aversa A. Lifestyle and fertility: The influence of stress and quality of life on male fertility. Reprod Biol Endocrinol 2018; 16: 115.
[17] Bräuner EV, Nordkap L, Priskorn L, Hansen AM, Bang AK, Holmboe SA, et al. Psychological stress, stressful life events, male factor infertility, and testicular function: A cross-sectional study. Fertil Steril 2020; 113: 865–875.
[18] Juruena MF, Eror F, Cleare AJ, Young AH. The role of early life stress in HPA axis and anxiety. Adv Exp Med Biol 2020; 1191: 141–153.
[2] Mishra P, Negi MPS, Srivastava M, Singh K, Rajender S. Decline in seminal quality in Indian men over the last 37 years. Reprod Biol Endocrinol 2018; 16: 103–104.
[3] Duan YG, Yu CF, Novak N, Bieber T, Zhu CH, Schuppe HC, et al. Immunodeviation towards a Th17 immune response associated with testicular damage in azoospermic men. Int J Androl 34: e536–e545.
[4] Qian LN, Shi Q, Gu Y, Song J, Zhou M, Hua M. The relationship between IL-17 and male infertility: Semen analysis. African J Microbiol Res 2012; 6: 5672–5677.
[5] Belardin LB, Antoniassi MP, Camargo M, Intasqui P, Fraietta R, Bertolla RP. Semen levels of matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinases (TIMP) protein families members in men with high and low sperm DNA fragmentation. Sci Rep 2019; 9: 903–904.
[6] Szarek M, Bergmann M, Konrad L, Schuppe HC, Kliesch S, Hedger MP, et al. Activin A target genes are differentially expressed between normal and neoplastic adult human testes: Clues to gonocyte fate choice. Andrology 2019; 7: 31–41.
[7] Ayvazova NP, Rilcheva VS, Konova EI, Roussev RG, Rashev PI. Matrix metalloproteinase MMP-2 and MMP-9 activities in seminal plasma. J Biomed Clin Res 2016; 9: 114–120.
[8] Nargund VH. Effects of psychological stress on male fertility. Nat Rev Urol 2015; 12: 373–382.
[9] Wirleitner B, Vanderzwalmen P, Stecher A, Spitzer D, Schuff M, Schwerda D, et al. Dietary supplementation of antioxidants improves semen quality of IVF patients in terms of motility, sperm count, and nuclear vacuolization. Int J Vitam Nutr Res 2012; 82: 391–398.
[10] Murray KS, James A, McGeady JB, Reed ML, Kuang WW, Nangia AK. The effect of the new 2010 world health organization criteria for semen analyses on male infertility. Fertil Steril 2012; 98: 1428–1431.
[11] Tentes I, Asimakopoulos B, Mourvati E, Diedrich K, Al- Hasani S, Nikolettos N. Matrix metalloproteinase (MMP)- 2 and MMP-9 in seminal plasma. J Assist Reprod Genet 2007; 24: 278–281.
[12] Nielsen MG, Ørnbøl E, Vestergaard M, Bech P, Larsen FB, Lasgaard M, et al. The construct validity of the perceived stress scale. J Psychosom Res 2016; 84: 22–30.
[13] Agarwal A, Rana M, Qiu E, Al Bunni H, Bui AD, Henkel R. Role of oxidative stress, infection and inflammation in male infertility. Andrologia 2018; 50: e13126.
[14] Sabbaghi M, Aram R, Roustaei H, Fadavi Islam M, Daneshvar M, Castaño AR, et al. IL-17A concentration of seminal plasma and follicular fluid in infertile men and women with various clinical diagnoses. Immunol Invest 2014; 43: 617–626.
[15] Lotti F, Tamburrino L, Marchiani S, Maseroli E, Vitale P, Forti G, et al. DNA fragmentation in two cytometric sperm populations: Relationship with clinical and ultrasound characteristics of the male genital tract. Asian J Androl 2017; 19: 272–279.
[16] Ilacqua A, Izzo G, Emerenziani GP, Baldari C, Aversa A. Lifestyle and fertility: The influence of stress and quality of life on male fertility. Reprod Biol Endocrinol 2018; 16: 115.
[17] Bräuner EV, Nordkap L, Priskorn L, Hansen AM, Bang AK, Holmboe SA, et al. Psychological stress, stressful life events, male factor infertility, and testicular function: A cross-sectional study. Fertil Steril 2020; 113: 865–875.
[18] Juruena MF, Eror F, Cleare AJ, Young AH. The role of early life stress in HPA axis and anxiety. Adv Exp Med Biol 2020; 1191: 141–153.