Reproductive toxicity potentials of methanolic extract of Portulaca oleracea in male rats: An experimental study
Background: Purslane is an edible widely distributed shrub and one of the herbs used in decoctions for the treatment of different ailments including infertility. However, there is a shortage of evidence to validate its reproductive effects.
Objective: To investigate the effect of methanolic extract of Portulaca oleracea (MEPO) on the reproductive system of male rats.
Materials and Methods: Fifteen 10-wk old male Wistar rats with an average weight of 183 gr were randomly divided into three groups (n = 5/each). Group A (the control group) received distilled water only; group B received 400 mg/kg MEPO; and group C received 800 mg/kg MEPO for 14 days. The animals fasted overnight after the 14th day of administration and euthanized by cervical dislocation. Blood samples, sperm, testes, and epididymis were collected for serum hormones, sperm, and histological analyses.
Results: There was no significant change in the serum luteinizing hormone and testosterone levels across all groups when compared to the control. However, group C showed a significant increase (p = 0.020) in follicle-stimulating hormone levels when compared to the control. There was a significant reduction (p = 0.006) in the sperm count in group C when compared with the control group. There was also a significantly reduced (p = 0.003) sperm motility in MEPO-treated groups compared to the control. While the testis showed no abnormalities in its histoarchitecture across groups, the epididymis showed some blood congestion in MEPO-treated groups.
Conclusion: Portulaca oleracea showed the ability to reduce sperm count and motility at higher doses.
Key words: Portulaca oleracea, Purslane, Testis, Epididymis, Rat, Sperm motility.
 Agarwal A, Aditi M, Alaa H, Michelle RC. A unique view on male infertility around the globe. Reprod Biol Endocrinol 2015; 13: 37.
 Hai Y, Hou J, Liu Y, Liu Y, Yang H, Li Z, et al. The roles and regulation of Sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis. Semin Cell Dev Biol 2014; 29: 66–75.
 Silber Sh. Fundamentals of male infertility. New York: Springer; 2018: 39–41.
 Hermo L, Pelletier RM, Cyr DG, Smith CE. Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 1: Background to spermatogenesis, spermatogonia, and spermatocytes. Microsc Res Tech 2010; 73: 241–278.
 WHO. WHO launches the first global strategy on traditional and alternative medicine. Available at: https://www.who.int/mediacentre/news/releases/ release38/en/. Accessed 21st January, 2021.
 Soetan KO, Aiyelaagbe OO. The need for bioactivitysafety evaluation and conservation of medicinal plants: A review. J Med Plants Res 2009; 3: 324–328.
 Okafor IA, Ezejindu DN. Phytochemical studies on portulaca oleracea (pourslane) plant. Glob J Biol Agr Health Sci 2014; 3: 132–136.
 Lanska D. The illustrated guide to edible plants. London: Chancellor Press; 2010.
 Okafor I, Ayalokunrin M, Orachu L. A review on Portulaca oleracea (Purslane) plant - Its nature and biomedical benefits. Int J Biomed Res 2014; 5: 75–80.
 Wong W. Some folk-medicinal plants from Trinidad. Economic Botany 1976; 30: 103–142.
 Dkhil M, Abdel-Moniem AE, Al-Quraishy S, Awadallah R. Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action. J Med Plant Res 2011; 5: 1589–1593.
 Okafor IA, Ejimofor OC, Ezejindu DN, Chukwujekwu IE, Dikeh CC. The renoprotective effect of methanolic extract of portulaca pleracea on cisplatin-induced nephrotoxicity in wistar rats. Adv Life Sci Technol 2014; 19: 52–60.
 Oyedeji KO, Bolarinwa AF. Effects of crude extracts of portulaca oleracea on male reproductive functions in albino rats. IOSR J Pharm Biol Sci 2013; 4: 71–79.
 Charan J, Kantharia ND. How to calculate sample size in animal studies? J Pharmacol Pharmacother 2013; 4: 303–306.
 Björndahl L, Mortimer D, Barratt CLR, Castilla JA, Menkveld R, Kvist U, et al. A practical guide to basic laboratory andrology. Cambridge: Cambridge University Press; 2010: 33–76.
 Geoffrey R. An Introduction to Specimen Processing. Available at: https://www.leicabiosystems.com/path ologyleaders/an-introduction-to-specimen-process ing/. Accessed 20th December; 2018.
 AccuBind ELISA Kit protocol sheet for Follicle stimulating hormone, Luteinizing hormone and Testosterone. https://www.monobind.com/Product s/immunoassays-fertility-prenatal-fsh.
 Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. PLOS Biol 2010; 8: e1000412.
 Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the care and use of Laboratory Animals. 8th Ed. Washington: National Academies Press (US); 2011.
 Ahangarpour A, Lamoochi Z, Fathi Moghaddam H, Mansouri SMT. Effects of portulaca olerace ethanolic extract on reproductive system of aging female mice. Int J Reprod Biomed 2016; 14: 205–212.
 Hosseini Seyed E, Frozanfar Far M, Payehdar A. The effect of hydroalcoholic extract of purslane (Portulaca oleracea L) on serum concentration of estrogen, progesterone, prolactin and gonadotropins in mature female rats. J Shahrekord Univ Med Sci 2013; 15: 12–21.
 Guyton A, Hall J. Textbook of Medical Physiology. 12th Ed. Philadelphia: Elsevier Saunders; 2011: 934– 941.
 Obinna VC, Kagbo HD, Agu GO. Effects of lipophilic and hydrophilic leaf extracts of portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. Am J Physiol Biochem Pharmacol 2019; 9: 21–32.