https://doi.org/10.18502/ijrm.v19i3.8571
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authors
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Behzad Abbasi
Behzad Abbasi
Trauma Research Center, AJA University of Medical Sciences, Tehran, Iran
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Homayoun Abbasi
Homayoun Abbasi
Isfahan Fertility and Infertility Center, Isfahan, Iran
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Hassan Niroumand
Hassan Niroumand
Trauma Research Center, AJA University of Medical Sciences, Tehran, Iran
Published Date
- Mar 21, 2021
Synbiotic (FamiLact) administration in idiopathic male infertility enhances sperm quality, DNA integrity, and chromatin status: A triple-blinded randomized clinical trial
Abstract
Background: Idiopathic male infertility is often treated empirically. A recent body of evidence has indicated the association between pro ± prebiotics administration and improvement in semen parameters.
Objective: To assess the effect of FamiLact (probiotic + prebiotic) administration on male subjects with idiopathic infertility.
Materials and Methods: Fifty-six men with idiopathic male infertility were randomly/equally divided into two groups. Men in the case and control groups received 500 mg of FamiLact and an identical placebo for 80 days, respectively. A semen sample was obtained from each of the participants before initiation and after the termination of the treatment course. Samples underwent regular semen analysis and were further analyzed to assess the level of DNA damage (sperm chromatin structure assay), oxidative stress (BODIPY C11 staining), and protamine deficiency (chromomycin-A3 staining) in spermatozoa.
Results: No significant difference was observed between the baseline values of both groups. After intervention, mean sperm concentration, motility, and normal morphology were significantly higher in the FamiLact group compared to the placebo group (p < 0.05). In the FamiLact receivers, we detected improvement regarding the following parameters: concentration, motility, abnormal morphology, sperm lipid peroxidation, and DNA fragmentation (p ≤ 0.02). Likewise, in the placebo group, we noticed a decrease in the post-medication mean value of DNA fragmentation (p = 0.03) while observing no significant difference regarding other parameters.
Conclusion: FamiLact administration improves sperm concentration, motility, and abnormal morphology and decrease sperm DNA damage, possibly through alleviating oxidative stress in the seminal fluid.
Key words: Male infertility, DNA fragmentation, Sperm, Synbiotics, Probiotics, Fertility agents.
References
[2] Jungwirth A, Diemer T, Kopa Z, Krausz C, Minhas S, Tournaye H. EAU Guidelines on male infertility. Netherland: European Association of Urology; 2019.
[3] Agarwal A, Parekh N, Panner Selvam MK, Henkel R, Shah R, Shery T. Male oxidative stress infertility (MOSI): Proposed terminology and clinical practice guidelines for management of idiopathic male infertility. World J Mens Health 2019; 37: 269–312.
[4] Martin-Hidalgo D, Bragado MJ, Batista R, Oliveira PF, Alves MG. Antioxidants and male fertility: From molecular studies to clinical evidence. Antioxidants 2019; 8: 89.
[5] Duca Y, Calogero AE, Cannarella R, Condorelli RA, La Vignera S. Current and emerging medical therapeutic agents for idiopathic male infertility. Expert Opin Pharmacother 2019; 20: 55–67.
[6] Maretti C, Cavallini G. The association of a probiotic with a prebiotic (Flortec, Bracco) to improve the quality/quantity of spermatozoa in infertile patients with idiopathic oligoasthenoteratospermia: A pilot study. Andrology 2017; 5: 439–444.
[7] Valcarce DG, Genovés S, Riesco MF, Martorell P, Herráez MP, Ramón D. Probiotic administration improves sperm quality in asthenozoospermic human donors. Benef Microbes 2017; 8: 193–206.
[8] Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document: The international scientific association for probiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 2014; 11: 506–514.
[9] Zegers-Hochschild F, Adamson GD, Dyer S, Racowsky C, Sokol R, Mouzon J de, et al. The international glossary on infertility and fertility care, 2017. Hum Reprod 2017; 32: 1786–1801.
[10] World Health Organization. WHO Laboratory manual for the examination and processing of human semen. 5th Ed. Cambridge: Cambridge University Press; 2010.
[11] Karbaschian Z, Mokhtari Z, Pazouki A, Kabir A, Hedayati M, Soleymanzadeh Moghadam S, et al. Probiotic supplementation in morbid obese patients undergoing one anastomosis gastric bypass-mini gastric bypass (OAGB-MGB) surgery: A randomized, double-blind, placebo-controlled, clinical trial. Obes Surg 2018; 28: 2874–2885.
[12] Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA. Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol Hum Reprod 2007; 13: 203–211.
[13] Sánchez B, Delgado S, Blanco−Míguez A, Lourenço A, Gueimonde M, Margolles A. Probiotics, gut microbiota, and their influence on host health and disease. Mol Nutr Food Res 2017; 61: 1600240.
[14] Rooks MG, Garrett WS. Gut microbiota, metabolites and host immunity. Nat Rev Immunol 2016; 16: 341–352.
[15] Tenorio-Jiménez C, Martínez-Ramírez MJ, Gil Á, Gómez- Llorente Z. Effects of probiotics on metabolic syndrome: A systematic review of randomized clinical trials. Nutrients 2020; 12: 124.
[16] Misra S, Mohanty D. Psychobiotics: A new approach for treating mental illness? Crit Rev Food Sci Nutr 2019; 59: 1230–1236.
[17] Liu Y, Tran DQ, Rhoads JM. Probiotics in disease prevention and treatment. J Clin Pharmacol 2018; 58 (Suppl.): S164–S179.
[18] Gibson GR, Hutkins R, Sanders ME, Prescott SL, Reimer RA, Salminen SJ, et al. Expert consensus document: The international scientific association for probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastroenterol Hepatol 2017; 14: 491.
[19] Quigley EMM. Prebiotics and probiotics in digestive health. Clin Gastroenterol Hepatol 2019; 17: 333–344.
[20] Valcarce DG, Riesco MF, Martínez-Vázquez JM, Robles V. Long exposure to a diet supplemented with antioxidant and anti-inflammatory probiotics improves sperm quality and progeny survival in the zebrafish model. Biomolecules 2019; 9: 338.
[21] Mazanko MS, Gorlov IF, Prazdnova EV, Makarenko MS, Usatov AV, Bren AB, et al. Bacillus probiotic supplementations improve laying performance, egg quality, hatching of laying hens, and sperm quality of roosters. Probiotics Antimicrob Proteins 2018; 10: 367–373.
[22] Dardmeh F, Alipour H, Gazerani P, van der Horst G, Brandsborg E, Nielsen HI. Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model. PLoS One 2017; 12: e0185964.
[23] Mehdinejad N, Imanpour MR, Jafari V. Combined or individual effects of dietary probiotic, pediococcus acidilactici and nucleotide on reproductive performance in goldfish (Carassius auratus). Probiotics Antimicrob Proteins 2019; 11: 233–238.
[24] Ibrahim HA, Zhu Y, Wu C, Lu C, Ezekwe MO, Liao SF, et al. Selenium-enriched probiotics improves murine male fertility compromised by high fat diet. Biol Trace Elem Res 2012; 147: 251–260.
[25] Agarwal A, Baskaran S, Selvam MK, Finelli R, Barbarosie C, Robert KA et al. Scientific landscape of oxidative stress in male reproductive research: A scientometric study. Free Radic Biol Med 2020; 156: 36–44.
[26] Baskaran S, Finelli R, Agarwal A, Henkel R. Reactive oxygen species in male reproduction: A boon or a bane? Andrologia 2020; e13577.
[27] dos Santos Hamilton TR, Assumpção MEODA. Sperm DNA fragmentation: Causes and identification. Zygote 2020; 28: 1–8.
[28] Rahiminia T, Farashahi Yazd E, Ghasemi-Esmailabad S, Talebi AR. Relation between sperm protamine transcripts with global sperm DNA methylation and sperm DNA methyltransferases mRNA in men with severe sperm abnormalities. Hum Fertil 2019; 1–7.
[29] Amaretti A, Di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A. Antioxidant properties of potentially probiotic bacteria: In vitro and in vivo activities. Appl Microbiol Biotechnol 2013; 97: 809–817.
[30] Zanoni S, Pompei A, Cordisco L, Amaretti A, Rossi M, Matteuzzi D. Growth kinetics on oligo- and polysaccharides and promising features of three antioxidative potential probiotic strains. J Appl Microbiol 2008; 105: 1266–1276.
[31] Wang BG, Xu HB, Xu F, Zeng ZL, Wei H. Efficacy of oral bifidobacterium bifidum ATCC 29521 on microflora and antioxidant in mice. Can J Microbiol 2016; 62: 249–262.