Download fulltext
HTML
Maria, A., Socrates, K., Theofanis, V., Zoe, P., Giorgos, T., Anna-Bettina, H., Katerina, C., Grigoris, G., Basil, T., Maria, S., & Alexandros, L. (2021). Influence of conception and delivery mode on stress response marker Oct4B1 and imprinted gene expression related to embryo development: A cohort study. International Journal of Reproductive BioMedicine (IJRM), 19(3), 217–226. https://doi.org/10.18502/ijrm.v19i3.8569
https://doi.org/10.18502/ijrm.v19i3.8569
statistics
- 246 Abstract Views
- 223 PDF Views
- 0 HTML Views
- Cited by 1 article
authors
-
Argyraki Maria
Argyraki Maria
Laboratory of Genetics, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
-
Katafigiotis Socrates
Katafigiotis Socrates
Laboratory of Genetics, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
-
Vavilis Theofanis
Vavilis Theofanis
Laboratory of Biology and Genetics, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
-
Papadopoulou Zoe
Papadopoulou Zoe
Laboratory of Biology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Ioannina, Greece
-
Tzimagiorgis Giorgos
Tzimagiorgis Giorgos
Laboratory of Biological Chemistry, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
-
Haidich Anna-Bettina
Haidich Anna-Bettina
Department of Hygiene and Epidemiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
-
Chatzimeletiou Katerina
Chatzimeletiou Katerina
Unit for Human Reproduction, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
-
Grimbizis Grigoris
Grimbizis Grigoris
Unit for Human Reproduction, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
-
Tarlatzis Basil
Tarlatzis Basil
Unit for Human Reproduction, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
-
Syrrou Maria
Syrrou Maria
Unit for Human Reproduction, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
-
Lambropoulos Alexandros
Lambropoulos Alexandros
Laboratory of Genetics, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece
Published Date
- Mar 21, 2021
Influence of conception and delivery mode on stress response marker Oct4B1 and imprinted gene expression related to embryo development: A cohort study
Abstract
Background: Recent scientific data support that the mode of conception and delivery may influence epigenetic regulation and therefore embryo development. Octamer-binding transcription factor 4-B1 (OCT4B1), a novel variant of OCT4 with yet unknown biological function, is suggested to have a potential role in mediating cellular stress response. Furthermore, Insulinlike Growth Factor 2 (IGF2), Mesoderm-specific Transcript (MEST) and paternally expressed gene 10 (PEG10) are genes known as imprinted and are regulated via means of epigenetic regulation. The influence of delivery mode and conception on epigenetic regulation is an active research field.
Objective: Our aim was to correlate the expression level of Oct4B1 and the expression and methylation level of IGF2, MEST, and PEG10 imprinted genes with the mode of delivery and conception in the umbilical cord blood of newborns.
Materials and Methods: Samples of umbilical cord blood from infants born after vaginal delivery, caesarean section (CS) with the infant in cephalic position and CS due to breech position were examined. Furthermore, the investigation included infants conceived through means of assisted reproductive technology.
Results: No statistically significant differences were found in mRNA expression levels between different modes of conception and delivery (p = 0.96). Oct4B1, IGF2, MEST, and PEG10 expression levels do not seem to be significantly affected by different modes of conception and delivery.
Conclusion: These results indicate that the expression and methylation patterns of Oct4B1, IGF2, MEST and PEG10 in umbilical cord blood are not affected by the conception and delivery mode.
Key words: Conception, Fertilization in vitro, Genomic imprinting, Fetal blood.
References
[1] Lagercrantz H. The good stress of being born. Acta Paediatr 2016; 105: 1413–1416.
[2] Anifandis G, Messini CI, Dafopoulos K, Messinis IE. Genes and conditions controlling mammalian pre- and post-implantation embryo development. Curr Genomics 2015; 16: 32–46.
[3] Canovas S, Ross PJ, Kelsey G, Coy P. DNA methylation in embryo development: Epigenetic impact of ART (assisted reproductive technologies). Bioessays 2017; 39: 1700106: 1–11.
[4] Chen J, Li Q, Rialdi A, Mystal E, Ly J, Finik J, et al. Influences of maternal stress during pregnancy on the epi/genome: Comparison of placenta and umbilical cord blood. J Depress Anxiety 2014; 3: 152–167.
[5] Sakian S, Louie K, Wong EC, Havelock J, Kashyap S, Rowe T, et al. Altered gene expression of H19 and IGF2 in placentas from ART pregnancies. Placenta 2015; 36: 1100–1105.
[6] Takeda J, Seino S, Bell GI. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res 1992; 20: 4613–4620.
[7] Farashahi Yazd E, Rafiee MR, Soleimani M, Tavallaei M, Kabir Salmani M, Mowla SJ. OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response. Cancer Lett 2011; 309: 170–175.
[8] Gao Y, Wei J, Han J, Wang X, Su G, Zhao Y, et al. The novel function of OCT4B isoform-265 in genotoxic stress. Stem Cells 2012; 30: 665– 672.
[9] Papamichos SI, Kotoula V, Tarlatzis BC, Agorastos T, Papazisis K, Lambropoulos AF. OCT4B1 isoform: The novel OCT4 alternative spliced variant as a putative marker of stemness. Mol Hum Reprod 2009; 15: 269–270.
[10] Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 2000; 24: 372–376.
[11] St-Pierre J, Hivert MF, Perron P, Poirier P, Guay SP, Brisson D, et al. IGF2 DNA methylation is a modulator of newborn’s fetal growth and development. Epigenetics 2012; 7: 1125–1132.
[12] Kappil MA, Green BB, Armstrong DA, Sharp AJ, Lambertini L, Marsit CJ, et al. Placental expression profile of imprinted genes impacts birth weight. Epigenetics 2015; 10: 842–849.
[13] Lim AL, Ng S, Leow SC, Choo R, Ito M, Chan YH, et al. Epigenetic state and expression of imprinted genes in umbilical cord correlates with growth parameters in human pregnancy. J Med Genet 2012; 49: 689–697.
[14] Kosaki K, Kosaki R, Craigen WJ, Matsuo N. Isoform-specific imprinting of the human PEG1/MEST gene. Am J Hum Genet 2000; 66: 309–312.
[15] Dimitriadou E, Noutsopoulos D, Markopoulos G, Vlaikou AM, Mantziou S, Traeger-Synodinos J, et al. Abnormal DLK1/MEG3 imprinting correlates with decreased HERV-K methylation after assisted reproduction and preimplantation genetic diagnosis. Stress 2013; 16: 689–697.
[16] Papamichos SI, Lambropoulos AF, Kotoula V. OCT4B expression in PBMNCs suggests the existence of an alternative OCT4 promoter. Genes Chromosomes Cancer 2009; 48: 1112– 1114.
[17] Mirzaei MR, Asadi MH, Mowla SJ, Hassanshahi G, Ahmadi Z. Down-regulation of HSP40 gene family following OCT4B1 suppression in human tumor cell lines. Iran J Basic Med Sci 2016; 19: 187–193.
[18] Asadzadeh J, Asadi MH, Shakhssalim N, Rafiee MR, Kalhor HR, Tavallaei M, et al. A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors. Urol J 2012; 9: 574–580.
[19] Poursani EM, Mehravar M, Shahryari A, Mowla SJ, Soltani BM. Alternative splicing generates different 5’ UTRs in OCT4B variants. Avicenna J Med Biotechnol 2017; 9: 201–204.
[20] Varrault A, Gueydan C, Delalbre A, Bellmann A, Houssami S, Aknin C, et al. Zac1 regulates an imprinted gene network critically involved in the control of embryonic growth. Dev Cell 2006; 11: 711–722.
[21] Fauque P, Ripoche MA, Tost J, Journot L, Gabory A, Busato F, et al. Modulation of imprinted gene network in placenta results in normal development of in vitro manipulated mouse embryos. Hum Mol Genet 2010; 19: 1779–1790.
[22] Choux C, Carmignac V, Bruno C, Sagot P, Vaiman D, Fauque P. The placenta: Phenotypic and epigenetic modifications induced by assisted reproductive technologies throughout pregnancy. Clin Epigenetics 2015; 7: 87–106.
[23] Choux C, Binquet C, Carmignac V, Bruno C, Chapusot C, Barberet J, et al. The epigenetic control of transposable elements and imprinted genes in newborns is affected by the mode of conception: ART versus spontaneous conception without underlying infertility. Hum Reprod 2018; 33: 331–340.
[24] Iglesias-Platas I, Martin-Trujillo A, Petazzi P, Guillaumet-Adkins A, Esteller M, Monk D. Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta. Hum Mol Genet 2014; 23: 6275–6285.
[2] Anifandis G, Messini CI, Dafopoulos K, Messinis IE. Genes and conditions controlling mammalian pre- and post-implantation embryo development. Curr Genomics 2015; 16: 32–46.
[3] Canovas S, Ross PJ, Kelsey G, Coy P. DNA methylation in embryo development: Epigenetic impact of ART (assisted reproductive technologies). Bioessays 2017; 39: 1700106: 1–11.
[4] Chen J, Li Q, Rialdi A, Mystal E, Ly J, Finik J, et al. Influences of maternal stress during pregnancy on the epi/genome: Comparison of placenta and umbilical cord blood. J Depress Anxiety 2014; 3: 152–167.
[5] Sakian S, Louie K, Wong EC, Havelock J, Kashyap S, Rowe T, et al. Altered gene expression of H19 and IGF2 in placentas from ART pregnancies. Placenta 2015; 36: 1100–1105.
[6] Takeda J, Seino S, Bell GI. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res 1992; 20: 4613–4620.
[7] Farashahi Yazd E, Rafiee MR, Soleimani M, Tavallaei M, Kabir Salmani M, Mowla SJ. OCT4B1, a novel spliced variant of OCT4, generates a stable truncated protein with a potential role in stress response. Cancer Lett 2011; 309: 170–175.
[8] Gao Y, Wei J, Han J, Wang X, Su G, Zhao Y, et al. The novel function of OCT4B isoform-265 in genotoxic stress. Stem Cells 2012; 30: 665– 672.
[9] Papamichos SI, Kotoula V, Tarlatzis BC, Agorastos T, Papazisis K, Lambropoulos AF. OCT4B1 isoform: The novel OCT4 alternative spliced variant as a putative marker of stemness. Mol Hum Reprod 2009; 15: 269–270.
[10] Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet 2000; 24: 372–376.
[11] St-Pierre J, Hivert MF, Perron P, Poirier P, Guay SP, Brisson D, et al. IGF2 DNA methylation is a modulator of newborn’s fetal growth and development. Epigenetics 2012; 7: 1125–1132.
[12] Kappil MA, Green BB, Armstrong DA, Sharp AJ, Lambertini L, Marsit CJ, et al. Placental expression profile of imprinted genes impacts birth weight. Epigenetics 2015; 10: 842–849.
[13] Lim AL, Ng S, Leow SC, Choo R, Ito M, Chan YH, et al. Epigenetic state and expression of imprinted genes in umbilical cord correlates with growth parameters in human pregnancy. J Med Genet 2012; 49: 689–697.
[14] Kosaki K, Kosaki R, Craigen WJ, Matsuo N. Isoform-specific imprinting of the human PEG1/MEST gene. Am J Hum Genet 2000; 66: 309–312.
[15] Dimitriadou E, Noutsopoulos D, Markopoulos G, Vlaikou AM, Mantziou S, Traeger-Synodinos J, et al. Abnormal DLK1/MEG3 imprinting correlates with decreased HERV-K methylation after assisted reproduction and preimplantation genetic diagnosis. Stress 2013; 16: 689–697.
[16] Papamichos SI, Lambropoulos AF, Kotoula V. OCT4B expression in PBMNCs suggests the existence of an alternative OCT4 promoter. Genes Chromosomes Cancer 2009; 48: 1112– 1114.
[17] Mirzaei MR, Asadi MH, Mowla SJ, Hassanshahi G, Ahmadi Z. Down-regulation of HSP40 gene family following OCT4B1 suppression in human tumor cell lines. Iran J Basic Med Sci 2016; 19: 187–193.
[18] Asadzadeh J, Asadi MH, Shakhssalim N, Rafiee MR, Kalhor HR, Tavallaei M, et al. A plausible anti-apoptotic role of up-regulated OCT4B1 in bladder tumors. Urol J 2012; 9: 574–580.
[19] Poursani EM, Mehravar M, Shahryari A, Mowla SJ, Soltani BM. Alternative splicing generates different 5’ UTRs in OCT4B variants. Avicenna J Med Biotechnol 2017; 9: 201–204.
[20] Varrault A, Gueydan C, Delalbre A, Bellmann A, Houssami S, Aknin C, et al. Zac1 regulates an imprinted gene network critically involved in the control of embryonic growth. Dev Cell 2006; 11: 711–722.
[21] Fauque P, Ripoche MA, Tost J, Journot L, Gabory A, Busato F, et al. Modulation of imprinted gene network in placenta results in normal development of in vitro manipulated mouse embryos. Hum Mol Genet 2010; 19: 1779–1790.
[22] Choux C, Carmignac V, Bruno C, Sagot P, Vaiman D, Fauque P. The placenta: Phenotypic and epigenetic modifications induced by assisted reproductive technologies throughout pregnancy. Clin Epigenetics 2015; 7: 87–106.
[23] Choux C, Binquet C, Carmignac V, Bruno C, Chapusot C, Barberet J, et al. The epigenetic control of transposable elements and imprinted genes in newborns is affected by the mode of conception: ART versus spontaneous conception without underlying infertility. Hum Reprod 2018; 33: 331–340.
[24] Iglesias-Platas I, Martin-Trujillo A, Petazzi P, Guillaumet-Adkins A, Esteller M, Monk D. Altered expression of the imprinted transcription factor PLAGL1 deregulates a network of genes in the human IUGR placenta. Hum Mol Genet 2014; 23: 6275–6285.