https://doi.org/10.18502/ijrm.v19i1.8184
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authors
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Zeynab Yazdanpanah
Zeynab Yazdanpanah
Department of Biology, Faculty of Science, Islamic Azad University Central Tehran branch, Tehran, Iran
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Mitra Heydari Nasrabadi
Mitra Heydari Nasrabadi
Department of Biology, Faculty of Biology Science, Islamic Azad University, Parand Branch, Tehran, Iran
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Zeynab Piravar
Zeynab Piravar
Department of Biology, Faculty of Science, Islamic Azad University Central Tehran branch, Tehran, Iran
Published Date
- Jan 25, 2021
Comparison of three sperm selection methods for ICSI-DGC, Cumulus column, and incubation with supernatant product of adipose tissue-derived adult stem cells: An experimental study
Abstract
Background: The examination of sperm parameters and sperm DNA integrity are necessary for male fertility expression. These parameters can be affected by the method of sperm separation.
Objective: To measure the damage of each sperm separation method on the sperm parameters and sperm DNA integrity.
Materials and Methods: In this experimental study, semen samples of 20 infertile men with asthenoteratozoospermia (Infertility Research Center, Qom, Iran, 2017) were processed in three ways: density gradient centrifugation (DGC), cumulus column, and incubation with supernatant products of adipose tissue-derived adult stem cells (SPAS). The results of sperm parameters and DNA fragmentation before and after the process were statistically analyzed.
Results: The number of separated sperms by normal morphologies during the SPAS and the cumulus column was significantly more than the corresponding population in the DGC group. In addition, although all three methods have the same ability to increase total sperm motility and the number of recovered sperms, in the field of forwarding movement and DNA fragmentation, the SPAS method performed more efficiently (p = 0.021).
Conclusion: Sperm parameters and DNA fragmentation in the SPAS group were better than those in the DGC and cumulus column groups. Furthermore, it has been shown that the sperm capacity was increased with the SPAS method. However, the rearrangement of sperm chromatin by reducing the disulfide bridges and providing the possibility of re-histone over capacity causes a significant reduction in DNA fragmentation.
Key words: Sperm, DNA fragmentation, Cumulus oophorus column, SPAS.
References
[2] Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol 2003; 1: 108–129.
[3] Dahan MH, Tan SL, Chung J, Son WY. Clinical definition paper on in vitro maturation of human oocytes. Hum Reprod 2016; 31: 1383–1386.
[4] Sills ES, Wittkowski KM, Tucker MJ, Perloe M, Kaplan CR, Palermo GD. Comparison of centrifugation and noncentrifugation based techniques for recovery of motile human spermatozoa in assisted reproduction. Arch Androl 2002; 48: 141–145.
[5] Cayli S, Jakab A, Ovari L, Delpiano E, Celik-Ozenci C, Sakkas D, et al. Biochemical markers of sperm function: male fertility and sperm selection for ICSI. Reprod Biomed Online 2003; 7: 462–468.
[6] World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5ed. Japane: Cambridge University Press; 2010.
[7] Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril 2005; 84: 833–842.
[8] Fazaeli H, Davoodi F, Kalhor N, Safarloo M, Masaii M, Ghiasi M, et al. Introducing of a new experimental method in semen preparation: Supernatant product of adipose tissue derived mesenchymal stem cells (SPAS). JFIV Reprod Med Genet 2016; 4: 1000178. 2–11.
[9] Rijsdijk M, Franken DR. Use of the capillary-cumulus oophorus model for evaluating the selection of spermatozoa. Fertil Steril 2007; 88: 1595–1602.
[10] Hong SJ, Chiu PC, Lee KF, Tse JMY, Ho PC, Yeung WSB. Establishment of a capillary ± cumulus model to study the selection of sperm for fertilization by the cumulus oophorus. Hum Reprod 2004; 19: 1562–1569.
[11] Selvarajua S, Sivasubramania T, Raghavendraa BS, Rajua P, Raob SBN, Dineshkumar D, et al. Effect of dietary energy on seminal plasma insulin-like growth factor-I (IGF-I), serum IGF-I and testosterone levels, semen quality and fertility in adult rats. Theriogenology 2012; 78: 646–655.
[12] Wiltshire EJ, Flaherty SP, Couper RTL. Hepatocyte growth factor in human semen and its association with semen parameters. Hum Reprod 2000; 15: 1525–1528.
[13] Breitbart H, Rotman T, Rubinstein S, Etkovitz N. Role and regulation of PI3K in sperm capacitation and the acrosome reaction. Mol Cell Endocrinol 2010; 314: 234–238.
[14] Sakkas D, Manicardi GC, Tomlinson M, Mandrioli M, Bizzaro D, Bianchi PG, et al. The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod 2000; 15: 1112– 1116.