https://doi.org/10.18502/ijrm.v19i1.8179
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authors
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Abdul Salam Ansari
Abdul Salam Ansari
Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India
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Kiran Sevliya
Kiran Sevliya
Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India
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Ayesha Badar
Ayesha Badar
Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India
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Nirmal Kumar Lohiya
Nirmal Kumar Lohiya
Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India
Published Date
- Jan 25, 2021
Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study
Abstract
Background: Reversible inhibition of sperm under guidance (“RISUG®”) is a promising intravasal male contraceptive.
Objective: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUG® in females.
Materials and Methods: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUG® for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUG® for 90 days and reversal with 5% NaHCO3. Animals were subjected to bilateral fallopian tube occlusion with RISUG® and reversal with DMSO and NaHCO3. The estrous cycle, fertility and histology of fallopian tube were evaluated.
Results: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUG® implant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups.
Conclusion: RISUG® is suitable for single intervention, intratubular, reversible contraception in female rats.
Key words: Contraception, Fallopian tube, Tubal occlusion, RISUG®, Reversible.
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