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Omidi, Z., Piravar, Z., & Ramezani, M. (2020). The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study. International Journal of Reproductive BioMedicine (IJRM), 13(10), 855–864. https://doi.org/10.18502/ijrm.v13i10.7770
https://doi.org/10.18502/ijrm.v13i10.7770
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Zeinab Omidi
Zeinab Omidi
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
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Zeinab Piravar
Zeinab Piravar
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
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Mina Ramezani
Mina Ramezani
Department of Biology, Faculty of Sciences, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Published Date
- Oct 14, 2020
The effect of acrylamide on mitochondrial membrane potential and glutathione extraction in human spermatozoa: A laboratory study
Abstract
Background: Acrylamide (AA) is a compound used in the industrial production of polyacrylamide. AAs affects by creating oxidative stress. It produces reactive oxygen species and leads to lipid peroxide. Lipid peroxidation in the cell membrane is one of the most important oxidations in the sperm, which can disrupt the fluidity and permeability of cell membranes and damage all cells.
Objective: To investigate the different concentrations of AA on human sperm parameters based on the World Health Organization standard and its impact on mitochondrial membrane potential and sperm glutathione levels.
Materials and Methods: In this laboratory study, we examined the different concentrations of AA on human sperm parameters based on the World Health Organization standard and its impact on mitochondrial membrane potential by flow cytometry and sperm glutathione levels by ELISA assay.
Results: The results were reported as the mean fluorescence intensity of JC and the index was observed to decrease following the effect of AA in mitochondrial membrane potential (Δ Ψm). The results of ELISA test to study the level of intracellular glutathione showed that with the increase in the concentration of AA exposed to sperms, there was a significant reduction in the level of intracellular glutathione.
Conclusion: AA destroys the sperm membrane integrity under apoptotic and oxidative inductions with a negative impact on mitochondrial function and antioxidative enzyme in sperm such as glutathione.
Key words: Acrylamides, Spermatozoa, Mitochondrial membrane potential, Glutathione S-Transferase.
References
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[12] Cooper TG, Moonan E, Eckardstein SV, Auger J, Gordon Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010; 16: 231–245.
[13] Ariagno JL, Mendeluk GR, Furlan MJ, Sardi M, Chenlo P, Curi SM, et al. Computer-aided sperm analysis: a useful tool to evaluate patient’s response to varicocelectomy. Asian J Androl 2017; 19: 449–452.
[14] Sivandzade F, Bhalerao A, Cucullo L. Analysis of the mitochondrial membrane potential using the cationic JC- 1 dye as a sensitive fluorescent probe. Bio Protec 2019; 9: e3128.
[15] Pourentezari M, Talebi A, Abbasi A, Khalili MA, Mangoli E, Anvari M. Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice. Iran J Reprod Med 2014; 12: 335–342.
[16] ALKarim S, ElAssouli S, Ali S, Ayuob N, ElAssouli Z. Effects of low dose acrylamide on the rat reproductive organs structure, fertility and gene integrity. Asian Pacific Journal of Reproduction 2015; 4: 179–187.
[17] Tyl RW, Marr MC, Myers CB, Ross WP, Friedman MA. Relationship between acrylamide reproductive and neurotoxicity in male rats. Reproductive Toxicology 2000; 14: 147–157.
[18] Ma Y, Shi J, Zheng M, Liu J, Tian S, He X, et al. Toxicological effects of acrylamide on the reproductive system of weaning male rats. Toxicol Ind Health 2011; 27: 617–627.
[19] Yilmaz BO, Yildizbayrak N, Aydin Y, Erkan M. Evidence of acrylamide- and glycidamide-induced oxidative stress and apoptosis in Leydig and Sertoli cells. Hum Exp Toxicol 2017; 36: 1225–1235.
[20] Gosalvez J, Tvrda E, Agarwal A. Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future. J Assist Reprod Genet 2017; 34: 697–707.
[21] He B, Guo H, Gong Y, Zhao R. Lipopolysaccharide-induced mitochondrial dysfunction in boar sperm is mediated by activation of oxidative phosphorylation. Theriogenology 2017; 87: 1–8.
[22] Agnihotri SK, Agrawal AK, Hakim BA, Vishwakarma AL, Narender T, Sachan R, et al. Mitochondrial membrane potential (MMP) regulates sperm motility. In Vitro Cell Dev Biol Anim 2016; 52: 953–960.
[23] Chai RR, Chen GW, Shi HJ, Wai-Sum O, Martin-DeLeon PA, Chen H. Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm. J Cell Mol Med 2017; 21: 121–129.
[24] Juárez-Rojas AL, García-Lorenzana M, Aragón-Martínez A, Gómez-Quiroz LE, Retana-Márquez Mdel S. Intrinsic and extrinsic apoptotic pathways are involved in rat testis by cold water immersion-induced acute and chronic stress. Syst Biol Reprod Med 2015; 61: 211–221.
[25] Nichi M, Rijsselaere T, Losano J, Angrimani D, Kawai G, Goovaerts I, et al. Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and in vitro fertilization in bovine epididymal sperm. Reprod Domest Anim 2017; 52: 257–263.
[26] Ahmad M, Ahmad N, Riaz A, Anzar M. Sperm survival kinetics in different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation. Reprod Fertil Dev 2015; 27: 784–793.
[27] Mughal IA, Irfan A, Hameed A, Jahan S. Sperm mitochondrial DNA 15bp deletion of cytochrome c oxidase subunit III is significantly associated with human male infertility in Pakistan. J Pak Med Assoc 2016; 66: 3–7.
[28] Barbonetti A, Castellini C, Di Giammarco N, Santilli G, Francavilla S, Francavilla F. In vitro exposure of human spermatozoa to bisphenol A induces prooxidative/ apoptotic mitochondrial dysfunction. Reprod Toxicol 2016; 66: 61–67.
[29] Malić Vončina S, Golob B, Ihan A, Kopitar AN, Kolbezen M, Zorn B. Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertil Steril 2016; 105: 637–644.
[30] Hering DM, Lecewicz M, Kordan W, Majewska A, Kaminski S. Missense mutation in glutathione-S-transferase M1 gene is associated with sperm motility and ATP content in frozen-thawed semen of Holstein-Friesian bulls. Anim Reprod Sci 2015; 159: 94–97.
[31] Silva JV, Freitas MJ, Correia BR, Korrodi-Gregório L, Patrício A, Pelech S, et al. Profiling signaling proteins in human spermatozoa: biomarker identification for sperm quality evaluation. Fertil Steril 2015; 104: 845– 856.
[2] Kumar J, Das S, Teoh SL. Dietary acrylamide and the risks of developing cancer: Facts to ponder. Front Nutr 2018; 5: 14–25.
[3] Azari A, Shokrzadeh M, Zamani E, Amani N, Shaki F. Cerium oxide nanoparticles protects against acrylamide induced toxicity in HepG2 cells through modulation of oxidative stress. Drug Chem Toxicol 2019; 42: 54–59.
[4] Najafi A, Amidi F, Sedighi Gilani MA, Moawad AR, Asadi E, Khanlarkhni N, et al. Effect of brain-derived neurotrophic factor on sperm function, oxidative stress and membrane integrity in human. Andrologia 2017; 49: 61–66.
[5] Santos Hamilton TRD, de Castro LS, Delgado Jde C, de Assis PM, Perez Siqueira AF, Mendes CM, et al. Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status. Reproduction 2016; 151: 379–390.
[6] Ferramosca A, Pinto Provenzano S, Montagna DD, Coppola L, Zara V. Oxidative stress negatively affects human sperm mitochondrial respiration. Urology 2013; 82: 78–83.
[7] Aitken RJ, Muscio L, Whiting S, Connaughton HS, Fraser BA, Nixon B, et al. Analysis of the effects of polyphenols on human spermatozoa reveals unexpected impacts on mitochondrial membrane potential, oxidative stress and DNA integrity; implications for assisted reproductive technology. Biochem Pharmacol 2016; 121: 78–96.
[8] Ling X, Zhang G, Sun L, Wang Z, Zou P, Gao J, et al. Polycyclic aromatic hydrocarbons exposure decreased sperm mitochondrial DNA copy number: A cross-sectional study (MARHCS) in Chongqing, China. Environ Pollut 2017; 220: 680–687.
[9] Treulen F, Uribe P, Boguen R, Villegas JV. Mitochondrial outer membrane permeabilization increases reactive oxygen species production and decreases mean sperm velocity but are not associated with DNA fragmentation in human sperm. Mol Hum Reprod 2016; 22: 83–92.
[10] Adeoye O, Olawumi J, Opeyemi A, Christiania O. Review on the role of glutathione on oxidative stress and infertility. JBRA Assist Reprod 2018; 22: 61–66.
[11] Kumar R, Singh VK, Atreja SK. Glutathione-S-transferase: role in buffalo (Bubalus bubalis) sperm capacitation and cryopreservation. Theriogenology 2014; 81: 587–598.
[12] Cooper TG, Moonan E, Eckardstein SV, Auger J, Gordon Baker HW, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010; 16: 231–245.
[13] Ariagno JL, Mendeluk GR, Furlan MJ, Sardi M, Chenlo P, Curi SM, et al. Computer-aided sperm analysis: a useful tool to evaluate patient’s response to varicocelectomy. Asian J Androl 2017; 19: 449–452.
[14] Sivandzade F, Bhalerao A, Cucullo L. Analysis of the mitochondrial membrane potential using the cationic JC- 1 dye as a sensitive fluorescent probe. Bio Protec 2019; 9: e3128.
[15] Pourentezari M, Talebi A, Abbasi A, Khalili MA, Mangoli E, Anvari M. Effects of acrylamide on sperm parameters, chromatin quality, and the level of blood testosterone in mice. Iran J Reprod Med 2014; 12: 335–342.
[16] ALKarim S, ElAssouli S, Ali S, Ayuob N, ElAssouli Z. Effects of low dose acrylamide on the rat reproductive organs structure, fertility and gene integrity. Asian Pacific Journal of Reproduction 2015; 4: 179–187.
[17] Tyl RW, Marr MC, Myers CB, Ross WP, Friedman MA. Relationship between acrylamide reproductive and neurotoxicity in male rats. Reproductive Toxicology 2000; 14: 147–157.
[18] Ma Y, Shi J, Zheng M, Liu J, Tian S, He X, et al. Toxicological effects of acrylamide on the reproductive system of weaning male rats. Toxicol Ind Health 2011; 27: 617–627.
[19] Yilmaz BO, Yildizbayrak N, Aydin Y, Erkan M. Evidence of acrylamide- and glycidamide-induced oxidative stress and apoptosis in Leydig and Sertoli cells. Hum Exp Toxicol 2017; 36: 1225–1235.
[20] Gosalvez J, Tvrda E, Agarwal A. Free radical and superoxide reactivity detection in semen quality assessment: past, present, and future. J Assist Reprod Genet 2017; 34: 697–707.
[21] He B, Guo H, Gong Y, Zhao R. Lipopolysaccharide-induced mitochondrial dysfunction in boar sperm is mediated by activation of oxidative phosphorylation. Theriogenology 2017; 87: 1–8.
[22] Agnihotri SK, Agrawal AK, Hakim BA, Vishwakarma AL, Narender T, Sachan R, et al. Mitochondrial membrane potential (MMP) regulates sperm motility. In Vitro Cell Dev Biol Anim 2016; 52: 953–960.
[23] Chai RR, Chen GW, Shi HJ, Wai-Sum O, Martin-DeLeon PA, Chen H. Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm. J Cell Mol Med 2017; 21: 121–129.
[24] Juárez-Rojas AL, García-Lorenzana M, Aragón-Martínez A, Gómez-Quiroz LE, Retana-Márquez Mdel S. Intrinsic and extrinsic apoptotic pathways are involved in rat testis by cold water immersion-induced acute and chronic stress. Syst Biol Reprod Med 2015; 61: 211–221.
[25] Nichi M, Rijsselaere T, Losano J, Angrimani D, Kawai G, Goovaerts I, et al. Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and in vitro fertilization in bovine epididymal sperm. Reprod Domest Anim 2017; 52: 257–263.
[26] Ahmad M, Ahmad N, Riaz A, Anzar M. Sperm survival kinetics in different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation. Reprod Fertil Dev 2015; 27: 784–793.
[27] Mughal IA, Irfan A, Hameed A, Jahan S. Sperm mitochondrial DNA 15bp deletion of cytochrome c oxidase subunit III is significantly associated with human male infertility in Pakistan. J Pak Med Assoc 2016; 66: 3–7.
[28] Barbonetti A, Castellini C, Di Giammarco N, Santilli G, Francavilla S, Francavilla F. In vitro exposure of human spermatozoa to bisphenol A induces prooxidative/ apoptotic mitochondrial dysfunction. Reprod Toxicol 2016; 66: 61–67.
[29] Malić Vončina S, Golob B, Ihan A, Kopitar AN, Kolbezen M, Zorn B. Sperm DNA fragmentation and mitochondrial membrane potential combined are better for predicting natural conception than standard sperm parameters. Fertil Steril 2016; 105: 637–644.
[30] Hering DM, Lecewicz M, Kordan W, Majewska A, Kaminski S. Missense mutation in glutathione-S-transferase M1 gene is associated with sperm motility and ATP content in frozen-thawed semen of Holstein-Friesian bulls. Anim Reprod Sci 2015; 159: 94–97.
[31] Silva JV, Freitas MJ, Correia BR, Korrodi-Gregório L, Patrício A, Pelech S, et al. Profiling signaling proteins in human spermatozoa: biomarker identification for sperm quality evaluation. Fertil Steril 2015; 104: 845– 856.