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Mohammadzadeh, M., Ali khalili, M., Ramezani, V., Hamishehkar, H., Dehghan Marvast, L., Mangoli, E., Rajabi, M., Akhavan Sales, Z., & Reza Talebi, A. (2020). Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study. International Journal of Reproductive BioMedicine (IJRM), 13(9), 755–764. https://doi.org/10.18502/ijrm.v13i9.7670
https://doi.org/10.18502/ijrm.v13i9.7670
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Masoomeh Mohammadzadeh
Masoomeh Mohammadzadeh
Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Mohammad Ali khalili
Mohammad Ali khalili
Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Vahid Ramezani
Vahid Ramezani
Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Hamed Hamishehkar
Hamed Hamishehkar
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Laleh Dehghan Marvast
Laleh Dehghan Marvast
Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Esmat Mangoli
Esmat Mangoli
Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Mahya Rajabi
Mahya Rajabi
Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Zhima Akhavan Sales
Zhima Akhavan Sales
Department of Immunology, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
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Ali Reza Talebi
Ali Reza Talebi
Department of Reproductive Biology, Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Published Date
- Sep 21, 2020
Does resveratrol affect prepared sperm parameters and chromatin quality in normozoospermic and asthenozoospermic patients before and after freezing? A lab trial study
Abstract
Background: Previous studies have examined the effect of resveratrol as a potent antioxidant for free radicals in semen. While, the prepared spermatozoa are more affected by ROS factors due to centrifugation and incubation.
Objective: To evaluate the RSV’s effects on the prepared sperm parameters and chromatin quality in both normozoospermic and asthenozoospermic cases before and after freezing.
Materials and Methods: The sample of 10 normozoospermic and asthenozoospermic men was prepared through the swim-up method. The groups were then divided into two samples of control and experimental (exposure to 30 μmol/l of RSV) to evaluate and compare the sperm parameters and chromatin quality before and after freezing.
Results: The motility and viability of spermatozoa were seen to be significantly different before and after freezing separately in the control and treatment samples of the groups (p ≤ 0.001 and p = 0.001, respectively). However, the stated difference between the control and treatment samples of normozoospermic and asthenozoospermic patients were not significant (p > 0.05). In addition, the sperm morphology and chromatin quality were not significantly different between the two samples of each group; nonetheless, chromatin quality of the treated sample was better than that of the control before and after freezing.
Conclusion: Despite the protective effects of RSV on the semen samples, RSV cannot affect significantly the prepared sperms parameters and chromatin quality in normozoospermic and asthenozoospermic patients.
Key words: Resveratrol, Chromatin, Motility, Spermatozoa, Freeze.
References
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[2] Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: an updated review of literature. Arab J Urol 2017; 16: 35–43.
[3] AL-Hady FN. The level of reactive oxygen species (ROS) in fresh and aged Asthenospermic, Leukocytospermic and Normospermic semen specimens. Al-Qadisiyah Journal of Veterinary Medicine Sciences 2006; 5: 42–48.
[4] Ko EY, Sabanegh Jr ES, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril 2014; 102: 1518–1527.
[5] Abdillah DA, Setyawan EMN, Oh HJ, Ra K, Lee SH, Kim MJ, et al. Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm. J Vet Sci 2019; 20: 79–86.
[6] Hezavehei M, Mohseni Kouchesfahani H, Shahverdi AH, Sharafi M, Hosseini Salekdeh GH, Eftekhari- Yazdi P. Induction of sublethal oxidative stress on human sperm before cryopreservation: A time-dependent response in post-thawed sperm parameters. Cell J 2019; 20: 537–543.
[7] Bansal AK, Bilaspuri GS. Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int 2010; 2010: 1–8.
[8] Chi HJ, Kim JH, Ryu CS, Lee JY, Park JS, Chung DY, et al. Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa. Hum Reprod 2008; 23: 1023–1028.
[9] Juan ME, Gonzalez-Pons E, Munuera T, Ballester J, Rodriguez-Gil JE, Planas JM. trans-resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats. J Nutr 2005; 135: 757–760.
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[11] Mangoli E, Talebi AR, Anvari M, Taheri F, Vatanparast M, Rahiminia T, et al. Vitamin C attenuates negative effects of vitrification on sperm parameters, chromatin quality, apoptosis and acrosome reaction in neat and prepared normozoospermic samples. Taiwan J Obstet Gynecol 2018; 57: 200–204.
[12] Matsumoto Y, Goto S, Hashimoto H, Kokeguchi S, Shiotani M, Okada H, et al. Andrology (Male Fertility, Spermatogenesis). Hum Reprod 2010; 25 (suppl.): i118–i152.
[13] Organization WH. WHO laboratory manual for the examination and processing of human semen. Switzerland, World Health Organization Press; 2010.
[14] Jayaraman V, Upadhya D, Narayan PK, Adiga SK. Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage. J Assist Reprod Genet 2012; 29: 557–563.
[15] Cui X, Jing X, Wu X, Yan M. Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity. Mol Med Rep 2016; 14: 4659–4665.
[16] Kruger TF, Ackerman SB, Simmons KF, Swanson RJ, Brugo SS, Acosta AA. A quick, reliable staining technique for human sperm morphology. Arch Androl 1987; 18: 275–277.
[17] Banihani S, Sharma R, Bayachou M, Sabanegh E, Agarwal A. Human sperm DNA oxidation, motility and viability in the presence of l−carnitine during in vitro incubation and centrifugation. Andrologia 2012; 44 (Suppl.): 505–512.
[18] Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245–251.
[19] Mangoli E, Khalili MA, Talebi AR, Ghasemi- Esmailabad S, Hosseini A. Is there any correlation between sperm parameters and chromatin quality with embryo morphokinetics in patients with male infertility? Andrologia 2018; 50: e12997.
[20] Di Santo M, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol 2012; 2012: 85837: 1–12.
[21] Tatone C, Di Emidio G, Vento M, Ciriminna R, Artini PG. Cryopreservation and oxidative stress in reproductive cells. Gynecol Endocrinol 2010; 26: 563–567.
[22] Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 2010; 60: 235–237.
[23] Collodel G, Federico MG, Geminiani M, Martini S, Bonechi C, Rossi C, et al. Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod Toxicol 2011; 31: 239–246.
[24] Tvrda E, Kovacik A, Tusimova E, Massanyi P, Lukac N. Resveratrol offers protection to oxidative stress induced by ferrous ascorbate in bovine spermatozoa. J Environ Sci Health A Tox Hazard Subst Environ Eng 2015; 50: 1440–1451.
[25] Meamar M, Zribi N, Cambi M, Tamburrino L, Marchiani S, Filimberti E, et al. Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica. Fertil Steril 2012; 98: 326–333.
[26] Dobrzynska MM. Resveratrol as promising natural radioprotector. A review. Rocz Państw Zakł Hig 2013; 64: 255–262.
[27] Garcez ME, dos Santos Branco C, Lara LV, Pasqualotto FF, Salvador M. Effects of resveratrol supplementation on cryopreservation medium of human semen. Fertil Steril 2010; 94: 2118–2121.
[2] Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: an updated review of literature. Arab J Urol 2017; 16: 35–43.
[3] AL-Hady FN. The level of reactive oxygen species (ROS) in fresh and aged Asthenospermic, Leukocytospermic and Normospermic semen specimens. Al-Qadisiyah Journal of Veterinary Medicine Sciences 2006; 5: 42–48.
[4] Ko EY, Sabanegh Jr ES, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril 2014; 102: 1518–1527.
[5] Abdillah DA, Setyawan EMN, Oh HJ, Ra K, Lee SH, Kim MJ, et al. Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm. J Vet Sci 2019; 20: 79–86.
[6] Hezavehei M, Mohseni Kouchesfahani H, Shahverdi AH, Sharafi M, Hosseini Salekdeh GH, Eftekhari- Yazdi P. Induction of sublethal oxidative stress on human sperm before cryopreservation: A time-dependent response in post-thawed sperm parameters. Cell J 2019; 20: 537–543.
[7] Bansal AK, Bilaspuri GS. Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int 2010; 2010: 1–8.
[8] Chi HJ, Kim JH, Ryu CS, Lee JY, Park JS, Chung DY, et al. Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa. Hum Reprod 2008; 23: 1023–1028.
[9] Juan ME, Gonzalez-Pons E, Munuera T, Ballester J, Rodriguez-Gil JE, Planas JM. trans-resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats. J Nutr 2005; 135: 757–760.
[10] Shabani Nashtaei M, Amidi F, Sedighi Gilani MA, Aleyasin A, Bakhshalizadeh Sh, Naji M, et al. Protective features of resveratrol on human spermatozoa cryopreservation may be mediated through 5’AMP−activated protein kinase activation. Andrology 2017; 5: 313–326.
[11] Mangoli E, Talebi AR, Anvari M, Taheri F, Vatanparast M, Rahiminia T, et al. Vitamin C attenuates negative effects of vitrification on sperm parameters, chromatin quality, apoptosis and acrosome reaction in neat and prepared normozoospermic samples. Taiwan J Obstet Gynecol 2018; 57: 200–204.
[12] Matsumoto Y, Goto S, Hashimoto H, Kokeguchi S, Shiotani M, Okada H, et al. Andrology (Male Fertility, Spermatogenesis). Hum Reprod 2010; 25 (suppl.): i118–i152.
[13] Organization WH. WHO laboratory manual for the examination and processing of human semen. Switzerland, World Health Organization Press; 2010.
[14] Jayaraman V, Upadhya D, Narayan PK, Adiga SK. Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage. J Assist Reprod Genet 2012; 29: 557–563.
[15] Cui X, Jing X, Wu X, Yan M. Protective effect of resveratrol on spermatozoa function in male infertility induced by excess weight and obesity. Mol Med Rep 2016; 14: 4659–4665.
[16] Kruger TF, Ackerman SB, Simmons KF, Swanson RJ, Brugo SS, Acosta AA. A quick, reliable staining technique for human sperm morphology. Arch Androl 1987; 18: 275–277.
[17] Banihani S, Sharma R, Bayachou M, Sabanegh E, Agarwal A. Human sperm DNA oxidation, motility and viability in the presence of l−carnitine during in vitro incubation and centrifugation. Andrologia 2012; 44 (Suppl.): 505–512.
[18] Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245–251.
[19] Mangoli E, Khalili MA, Talebi AR, Ghasemi- Esmailabad S, Hosseini A. Is there any correlation between sperm parameters and chromatin quality with embryo morphokinetics in patients with male infertility? Andrologia 2018; 50: e12997.
[20] Di Santo M, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol 2012; 2012: 85837: 1–12.
[21] Tatone C, Di Emidio G, Vento M, Ciriminna R, Artini PG. Cryopreservation and oxidative stress in reproductive cells. Gynecol Endocrinol 2010; 26: 563–567.
[22] Branco CS, Garcez ME, Pasqualotto FF, Erdtman B, Salvador M. Resveratrol and ascorbic acid prevent DNA damage induced by cryopreservation in human semen. Cryobiology 2010; 60: 235–237.
[23] Collodel G, Federico MG, Geminiani M, Martini S, Bonechi C, Rossi C, et al. Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod Toxicol 2011; 31: 239–246.
[24] Tvrda E, Kovacik A, Tusimova E, Massanyi P, Lukac N. Resveratrol offers protection to oxidative stress induced by ferrous ascorbate in bovine spermatozoa. J Environ Sci Health A Tox Hazard Subst Environ Eng 2015; 50: 1440–1451.
[25] Meamar M, Zribi N, Cambi M, Tamburrino L, Marchiani S, Filimberti E, et al. Sperm DNA fragmentation induced by cryopreservation: new insights and effect of a natural extract from Opuntia ficus-indica. Fertil Steril 2012; 98: 326–333.
[26] Dobrzynska MM. Resveratrol as promising natural radioprotector. A review. Rocz Państw Zakł Hig 2013; 64: 255–262.
[27] Garcez ME, dos Santos Branco C, Lara LV, Pasqualotto FF, Salvador M. Effects of resveratrol supplementation on cryopreservation medium of human semen. Fertil Steril 2010; 94: 2118–2121.