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Darbandi, S., Darbandi, M., Agarwal, A., Reza Khorram khorshid, H., Reza Sadeghi, M., Esteves, S., Sengupta, P., Dutta, S., Fathi, Z., Zeraati, H., & Mehdi Akhondi, M. (2020). Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study. International Journal of Reproductive BioMedicine (IJRM), 13(6), 425–438. https://doi.org/10.18502/ijrm.v13i6.7284

https://doi.org/10.18502/ijrm.v13i6.7284

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authors

  • Sara Darbandi

    Sara Darbandi

    Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
  • Mahsa Darbandi

    Mahsa Darbandi

    Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
  • Ashok Agarwal

    Ashok Agarwal

    American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, Ohio, USA.
  • Hamid Reza Khorram khorshid

    Hamid Reza Khorram khorshid

    Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
  • Mohammad Reza Sadeghi

    Mohammad Reza Sadeghi

    Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
  • Sandro Esteves

    Sandro Esteves

    Androfert, Andrology and Human Reproduction Clinic, Campinas, Brazil.
  • Pallav Sengupta

    Pallav Sengupta

    Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia.
  • Sulagna Dutta

    Sulagna Dutta

    Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia.
  • Zohreh Fathi

    Zohreh Fathi

    Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
  • Hojjat Zeraati

    Hojjat Zeraati

    Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohammad Mehdi Akhondi

    Mohammad Mehdi Akhondi

    Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Published Date

  • Jul 2, 2020

Comparing four laboratory three-parent techniques to construct human aged non-surrounded nucleolus germinal vesicle oocytes: A case-control study

International Journal of Reproductive BioMedicine (IJRM) / International Journal of Reproductive BioMedicine (IJRM): Volume 18, Issue No. 6 / Pages 425–438

Abstract

Background: The three-parent assisted reproductive technique may increase oocyte competence.


Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated.


Materials and Methods: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups.


Results: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2- cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4- cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001).


Conclusion: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes.


Key words: Assisted reproductive techniques, In vitro oocyte maturation techniques, Nuclear transfer techniques, Oocytes, Oocyte donation.

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