The effects of the Lactobacillus acidophilus ATCC 4356 on the oxidative stress of reproductive system in diabetic male rats
Background: Oxidative stress plays an important role in the development of diabetic complications.
Objective: This study evaluated the impact of pre- and post-treatment with Lactobacillus acidophilus ATCC 4356 on the oxidant and anti-oxidant factors of testis and epididymis in streptozotocin-induced diabetic rats.
Materials and Methods: Thirty male Wistar rats (10 wk old) weighing 220-230 g. were divided into five groups (n = 6/ each): 1- normal group, 2- normal lactobacillus group, 3- diabetic group, 4- diabetic + lactobacillus before (DLB) group, and 5- diabetic + lactobacillus after (DLA) group. The normal and diabetic groups received daily 1 mL normal saline for 6 wk. Normal lactobacillus group received daily L. acidophilus for 6 wk. Group DLB received daily L. acidophilus for 2 wk before diabetes and for 4 wk after diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes and L. acidophilus for 4 wk after diabetes. The dose of L. acidophilus was 1 × 109 CFU/mL.
Results: The administration of L. acidophilus worsened blood glucose level and reduced the levels of Malondialdehyde (p = ≤ 0.0001) and Hydrogen peroxide (p ≤ 0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did not show any changes compared to the diabetic rats. Also, there was no significant difference between DLB and DLA groups, in these markers.
Conclusion: Data obtained suggests that L. acidophilus has anti-oxidant effects on the testis and sometime in the epididymis in diabetic rats.
 Ribeiro MC, Nakamura MU, Torloni MR, Scanavino Mde T, Scomparini FB, Mattar R. Female sexual function of
overweight women with gestational diabetes mellitus - a cross-sectional study. PloS One 2014; 9: e95094.
 Trutnovsky G, Haas J, Lang U, Petru E. Women’s perception of sexuality during pregnancy and after birth. Aust N Z J Obstet Gynaecol 2006; 46: 282–287.
 Seven M, Akyuz A, Gungor S. Predictors of sexual function during pregnancy. J Obstet Gynaecol 2015; 35: 691–695.
 Jamali S, Mosalanejad L. Sexual dysfnction in Iranian pregnant women. Iran J Reprod Med 2013; 11: 479–486.
 Yeniel AO, Petri E. Pregnancy, childbirth, and sexual function: perceptions and facts. Int Urogynecol J 2014; 25:
 Williamson M, McVeigh C, Baafi M. An australian perspective of fatherhood and sexuality. Midwifery 2008; 24: 99–107.
 Bartellas E, Crane JM, Daley M, Bennett KA, Hutchens D. Sexuality and sexual activity in pregnancy. BJOG 2000;
 Mozafari M, Khajavikhan J, Jaafarpour M, Khani A, Direkvand-Moghadam A, Najafi F. Association of body
weight and female sexual dysfunction: a case control study. Iran Red Crescent Med J 2015; 17: e24685.
 Ramezani Tehrani F, Farahmand M, Simbar M, Malek Afzali H. Factors associated with sexual dysfunction; a population based study in Iranian reproductive age women. Arch Iran Med 2014; 17: 679–684.
 Weaver AD, Byers ES. The relationships among body image, body mass index, exercise, and sexual functioning in heterosexual women. Psychol Women Quarter 2006; 30: 333–339.
 Afshari P, Houshyar Z, Javadifar N, Pourmotahari F, Jorfi M. The relationship between body image and sexual function in middle-aged women. Electron Physician 2016; 8: 3302– 3308.
 Widen EM, Gallagher D. Body composition changes in pregnancy: measurement, predictors and outcomes. Eur J Clin Nutr 2014; 68: 643–652.
 Fuller-Tyszkiewicz M, Skouteris H, Watson BE, Hill B. Body dissatisfaction during pregnancy: a systematic review of cross-sectional and prospective correlates. J Health Psychol 2013; 18: 1411–1421.
 Manaf NA, Saravanan C, Zuhrah B. The prevalence and inter-relationship of negative body image perception,
depression and susceptibility to eating disorders among female medical undergraduate students. J Clin Diagn Res 2016; 10: VC01–VC04.
 Rahmani A, Sayehmiri K, Asadollahi K, Sarokhani D, Islami F, Sarokhani M. Investigation of the prevalence of obesity in iran: a systematic review and meta-analysis study. Acta Med Iran 2015; 53: 596–607.
 Felisbino-Mendes MS, Matozinhos FP, Miranda JJ, Villamor E, Velasquez-Melendez G. Maternal obesity and
fetal deaths: results from the Brazilian cross-sectional Demographic Health Survey, 2006. BMC Pregnancy
Childbirth 2014; 14: 5–15.
 Ribeiro MC, Nakamura MU, Torloni MR, Scanavino Mde T, Mancini PE, Forte BM, et al. Maternal overweight and sexual function in pregnancy. Acta Obstet Gynecol Scand 2016; 95: 45–51.
 Rosen R, Brown C, Heiman J, Leiblum S, Meston C, Shabsigh R, et al. The female sexual function index
(fsfi): a multidimensional self-report instrument for the assessment of female sexual function. J Sex Marit Ther
2000; 26: 191–208.
 Mohammadi Kh, Heydari M, Faghihzadeh S. [The female sexual function index (FSFI): validation of the Iranian version]. Payesh 2008; 7: 269–278. (in Persian)
 Cash TF. The body image work book (An 8-step program for learning to like your looks). Oakland: New Harbinger Publications; 1997.
 Rahati A. Evolutionary study of body image and its relationship with self-esteem based on comparison between adolescent, middle age and old people. Tehran: Shahed University; 2004.
 Shojaa M, Jouybari L, Sanagoo A. The sexual activity during pregnancy among a group of Iranian women. Arch Gynecol Obstet 2009; 279: 353–356.
 Leite AP, Campos AA, Dias AR, Amed AM, De Souza E, Camano L. Prevalence of sexual dysfunction during
pregnancy. Rev Assoc Med Bras 2009; 55: 563–568.
 Bayrami R, Sattarzadeh N, Ranjbar Koochaksariie F, Zakaria Pezeshki M. [Sexual dysfunction in couples and its related factors during pregnancy.] J Reprod Infertil 2008; 9: 271–283. (in Persian)
 Hayes RD, Dennerstein L, Bennett CM, Sidat M, Gurrin LC, Fairley CK. Risk factors for female sexual dysfunction in the general population: exploring factors associated with low sexual function and sexual distress. J Sex Med 2008; 5: 1681–1693.
 Tasdemir N, Celik C, Abali R, Ozer I, Gokhan Culha M, Serefoglu EC. Sexual function may be impaired during
pregnancy in adolescent women. Sexual and Relationship Therapy 2017; 32: 173–180.
 Naldoni LM, Pazmino MA, Pezzan PA, Pereira SB, Duarte G, Ferreira CH. Evaluation of sexual function in Brazilian pregnant women. J Sex Marital Ther 2011; 37: 116–129.
 Sossah L. Sexual behavior during pregnancy: A descriptive correlational study among pregnant women. Eur J Res Med Sci 2014; 2: 16–27.
 Faridi H, Najar Sh, Javadnoori M. The relationship between body mass index and women sexual function. Iran J Obstet Gynaecol Infertil 2013; 16: 20–28.
 Abidin A, Draman N, Ismail S, Mustaffa I, Ahmad I. Female sexual dysfunction among overweight and obese women in Kota Bharu, Malaysia. J Taibah Uni Med Sci 2016; 11: 159–167.
 Esposito K, Ciotola M, Giugliano F, Bisogni C, Schisano B, Autorino R, et al. Association of body weight with sexual function in women. Int J Impot Res 2007; 19: 353–357.
 Pauls RN, Occhino JA, Dryfhout VL. Effects of pregnancy on female sexual function and body image: a prospective study. J Sex Med 2008; 5: 1915–1922.
 Andersen BL, Legrand J. Body image for women: conceptualization, assessment, and a test of its importance to sexual dysfunction and medical illness. J Sex Res 1991; 28: 457–477.
 van den Brink F, Smeets MA, Hessen DJ, Woertman L. Positive body image and sexual functioning in dutch
female university students: the role of adult romantic attachment. Arch Sex Behav 2016; 45: 1217–1226.
 Woertman L, van den Brink F. Body image and female sexual functioning and behavior: a review. J Sex Res 2012;49: 184–211.
 Satinsky S, Reece M, Dennis B, Sanders S, Bardzell S. An assessment of body appreciation and its relationship to sexual function in women. Body Image 2012; 9: 137–144.
 Pujols Y, Seal BN, Meston CM. The association between sexual satisfaction and body image in women. J Sex Med 2010; 7: 905–916.
 Uwapusitanon W, Choobun T. Sexuality and sexual activity in pregnancy. J Med Assoc Thai 2004; 87 (Suppl.): 45–49.
 Heidari M, Amin Shokravi F, Zayeri F, Azin SA, MerghatiKhoei E. Sexual life during pregnancy: effect of an educational intervention on the sexuality of iranian couples: a quasiexperimental study. J Sex Marital Ther 2018; 44: 45–55.
 Afshar M, Mohammad-Alizadeh-Charandabi S, MerghtiKhoei ES, Yavarikia P. The effect of sex education on the sexual function of women in the first half of pregnancy: a randomized controlled trial. J Caring Sci 2012; 1: 173–181.