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Watcho, P., Lih, F., Brice Defo Deeh, P., Wankeu-Nya, M., Ngadjui, E., Romeo Fozing Bonsou, G., Kamanyi, A., & Kamtchouing, P. (2019). Aphrodisiac property of aqueous and methanolic extracts of Raphia vinifera (Arecaceae) in sexually experienced male rats. International Journal of Reproductive BioMedicine (IJRM), 17(6), 413–424. https://doi.org/10.18502/ijrm.v17i6.4813
https://doi.org/10.18502/ijrm.v17i6.4813
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authors
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Pierre Watcho
Pierre Watcho
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon
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Fred Lih
Fred Lih
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon
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Patrick Brice Defo Deeh
Patrick Brice Defo Deeh
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon
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Modeste Wankeu-Nya
Modeste Wankeu-Nya
Laboratory of Animal Biology and Physiology, Department of Animal Organisms Biology, University of Douala Douala, Cameroon
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Esther Ngadjui
Esther Ngadjui
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon
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Georges Romeo Fozing Bonsou
Georges Romeo Fozing Bonsou
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon
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Albert Kamanyi
Albert Kamanyi
Department of Animal Biology, Faculty of Science, Animal Physiology and Phytopharmacology Laboratory, University of Dschang, Dschang, Cameroon
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Pierre Kamtchouing
Pierre Kamtchouing
Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé, Yaoundé, Cameroon
Published Date
- Jul 29, 2019
Aphrodisiac property of aqueous and methanolic extracts of Raphia vinifera (Arecaceae) in sexually experienced male rats
Abstract
Background: Raphia vinifera (Arecaceae) is a medicinal plant commonly used as a sexual enhancer.
Objective: To investigate the aphrodisiac potential of aqueous extract (AE) and methanolic extract (ME) of R. vinifera in sexually experienced male rats.
Materials and Methods: Thirty male Wistar rats were randomly distributed into six groups (5 rats per group) and administered for 14 days with distilled water (10 ml/kg), sildenafil citrate (1.44 mg/kg), and AE or ME of R. vinifera (100 or 500 mg/kg). The copulatory activity was tested on days 0, 7, and 14 using receptive females. Further, on day 14, rats were sacrificed and biochemical analyses (testosterone, total protein, and acid phosphatase) were performed.
Results: Sildenafil citrate significantly decreased the intromission latency (day 14, p = 0.04) and frequency (days 7 and 14, p = 0.03) but increased the mount frequency (day 14, p = 0.04), compared with control. Remarkably, R. vinifera enhanced the sexual activity by significantly decreasing the intromission latency (AE and ME, 500 mg/kg, day 14, p = 0.04) and increasing the mount frequency (AE and ME, 100 mg/kg, day 7, p = 0.02) compared with control. Moreover, R. vinifera improved plasmatic (AE, 100 mg/kg, p = 0.03; AE, 500 mg/kg, p = 0.001; ME, 100 mg/kg, p = 0.01) and testicular (AE, 100 mg/kg, p = 0.001; AE, 500 mg/kg, p = 0.01; ME, 100 mg/kg, p = 0.001; ME, 500 mg/kg, p = 0.01) testosterone levels as well as plasmatic total proteins concentration (ME, 500 mg/kg, p = 0.04).
Conclusion: These findings showed that R. vinifera possesses an aphrodisiac property which could further justify its folkloric use in traditional medicine as a sexual enhancer.
References
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Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high fat diet-induced obese sexually sluggish rats. Asian Pac J Reprod 2017; 6: 202–211.
[13] Council of the European Union. Council Directive 86/609/EEC of 24 November 1986 on the approximation
of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Off J Eur Commun 1986; 358: 1–29.
[14] Carro-Juárez M, Cervantes E, Cervantes-Méndez M, Rodríguez-Manzo G. Aphrodisiac properties of Montanoa
tomentosa aqueous crude extract in male rats. Pharmacol Biochem Behav 2004; 78:129–134.
[15] Preedapirom W, Changwichit K, Srisawang P, Ingkaninan K, Taepavarapruk P. Aphrodisiac activity of Eulophia macrobulbon extract on erectile dysfunction in male aged rats. Biomed Res Int 2018; 2018: 6217029.
[16] Sandroni P. Aphrodisiacs past and present: a historical review. Clin Auton Res 2001; 11: 303–307.
[17] Erhabor JO, Idu M. Aphrodisiac potentials of the ethanol extract of Aloe barbadensis Mill. root in male Wistar rats.BMC Complement Altern Med 2017; 17: 360–369.
[18] Yakubu MT, Afolayan AJ. Effect of aqueous extract of Bulbine natalensis (Baker) stem on the sexual behaviour of male rats. Int J Androl 2009; 32: 629–636.
[19] Ratnasooriya WD, Dharmasiri MG. Effects of Terminalia catappa seeds on sexual behaviour and fertility of male rats. Asian J Androl 2000; 2: 213–219.
[20] Fouche G, Afolayan AJ, Wintola OA, Khorombi TE, Senabe J. Effect of the aqueous extract of the aerial parts of
Monsonia angustifolia E. Mey. Ex A. Rich., on the sexual behaviour of male Wistar rats. BMC Complement Altern
Med 2015; 15: 343.
[21] Kale OE, Awodele O, Akindele AJ. Acridocarpus smeathmannii (DC.) Guill. & Perr. Root enhanced reproductive behavior and sexual function in male wistar rats: Biochemical and pharmacological mechanisms. J Ethnopharmaco 2019; 230: 95–108.
[22] Andric SA, Janjic MM, Stojkov NJ, Kostic TS. Sildenafil treatment in vivo stimulates Leydig cell steroidogenesis
via the cAMP/cGMP signaling pathway. Am J Physiol Endocrinol Metab 2010; 299: E544–550.
[23] Besong EB, Ateufack G, Babiaka SB, Kamanyi A. Leafmethanolic extract of Pseudopanax arboreus (Araliaceae) (L. F. Phillipson) reverses amitriptyline-induced sexual dysfunction in male rats. Biochem Res Int 2018; 2018: 2869727.
[24] Zade V, Dabhadkar D, Thakare V, Pare S. Evaluation of potential aphrodisiac activity of Moringa oleifera seed in male albino rats. Int J Pharm Pharm Sci 2013; 5: 683–689.
[25] Gayrard V. Physiologie de la reproduction des mammifères. Ecole Nationale Vétérinaire, Toulouse 2007; 198.
[26] Gupta RS, Kachhawa JB, Chaudhary R. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats. Asian J Androl 2004; 6: 155–159.
[27] Chung HJ, Noh Y, Kim MS, Jang A, Lee CE, Myung SC. Steroidogenic effects of Taraxacum officinale extract on
the levels of steroidogenic enzymes in mouse Leydig cells. Anim Cells Syst (Seoul) 2018; 22: 407–414.
[28] Muniyan S, Chaturvedi NK, Dwyer JG, LaGrange CA, Chaney WG, Lin MF. Human prostatic acid phosphatase:
Structure, function and regulation. Int J Mol Sci 2013; 14: 10438–10464.
[29] Jian Feng C, Peng Ying Z, Cheng Wei X, Tao Tao H, Yun Gui B, Kao Shan C. Effect of aqueous extract of Arctium lappa L. (burdock) roots on the sexual behavior of male rats. BMC Complement Altern Med 2012; 12: 1–8.
[30] Hnatyszyn O, Moxatelli V, Rondina R, Costa M, Arranz C, Belaszczuk A, et al. Flavonoids from Achyzocline
satureiodes with relaxant effects on the smooth muscle in Guinea pig corpus cavernosum. Phytomedicine 2004; 11: 366–369.
[31] Kim HJ, Woo DS, Lee G, Kim JJ. The relaxation effects of ginseng saponin in rabbit corporal smooth muscle, is it a nitric oxide donor? Br J Urol 1998; 82: 744–748.
[2] Oboh G, Adebayo AA, Ademosun AO, Abegunde OA. Aphrodisiac effect of Hunteria umbellata seed extract:
Modulation of nitric oxide level and arginase activity in vivo. Pathophysiology 2019; 26: 39–47.
[3] Pfaff DW, Baum MJ. Hormone-dependent medial preoptic/lumbar spinal cord/autonomic coordination supporting male sexual behaviors. Mol Cell Endocrinol 2018; 467: 21–30.
[4] Moreira SG Jr, Brannigan RE, Spitz A, Orejuela FJ, Lipshultz LI, Kim ED. Side-effect profile of sildenafil citrate (Viagra)in clinical practice. Urology 2000; 56: 474–476.
[5] Watcho P, Modeste WN, Albert K, Carro-Juarez M. Dracaena arborea extracts delay the pro-ejaculatory effect of dopamine and oxytocin in spinal male rats. Int J Impot Res 2014; 26: 213–217.
[6] Deeh Defo PB, Asongu E, Wankeu MN, Ngadjui E, Bonsou Fazin GR, Kemka XF et al. Guibourtia tessmannii-induced fictive ejaculation in spinal male rat: involvement of D1, D2- like receptors. Pharm Biol 2017; 55: 1138 1143.
[7] Watcho P, Meli Watio H, Wankeu-Nya M, Ngadjui E, Deeh Defo P, Nkeng-Efouet AP, et al. Androgenic effects of
aqueous and methanolic extracts of Ficus asperifolia in male Wistar rats. BMC Complement Altern Med 2017; 17:
42.
[8] Nicodème Rodrigue ST, Ebénézer N, Dieunedort N, Didier F, Fogue M, Drean JY, et al. Investigation of the physical and mechanical properties of Raffia vinifera fibers along the stem. J Nat Fibers 2017. 14; 5: 621–633.
[9] Gruca M, Van Andel TR, Balslev H. Ritual uses of palms in traditional medicine in sub-Saharan Africa: a review. J Ethnobiol Ethnomed 2014; 10: 60.
[10] Fafioye OO. Plants with piscicidal activities in southwestern Nigeria. Turk J Fish Aquat Sci 2005; 5: 91–97.
[11] Meyerson BJ, Lindstrom LH. Sexual motivation in the female rat. A methodological study applied to the investigation of the effect of estradiol benzoate. Acta Physiol Scand Suppl 1973; 389: 1–80.
[12] Deeh-Defo PB, Wankeu-Nya M, Ngadjui E, Fozin BGR, Kemka FX, Kamanyi A, et al. The methanolic extract of
Guibourtia tessmannii (Caesalpiniaceae) improves sexual parameters in high fat diet-induced obese sexually sluggish rats. Asian Pac J Reprod 2017; 6: 202–211.
[13] Council of the European Union. Council Directive 86/609/EEC of 24 November 1986 on the approximation
of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes. Off J Eur Commun 1986; 358: 1–29.
[14] Carro-Juárez M, Cervantes E, Cervantes-Méndez M, Rodríguez-Manzo G. Aphrodisiac properties of Montanoa
tomentosa aqueous crude extract in male rats. Pharmacol Biochem Behav 2004; 78:129–134.
[15] Preedapirom W, Changwichit K, Srisawang P, Ingkaninan K, Taepavarapruk P. Aphrodisiac activity of Eulophia macrobulbon extract on erectile dysfunction in male aged rats. Biomed Res Int 2018; 2018: 6217029.
[16] Sandroni P. Aphrodisiacs past and present: a historical review. Clin Auton Res 2001; 11: 303–307.
[17] Erhabor JO, Idu M. Aphrodisiac potentials of the ethanol extract of Aloe barbadensis Mill. root in male Wistar rats.BMC Complement Altern Med 2017; 17: 360–369.
[18] Yakubu MT, Afolayan AJ. Effect of aqueous extract of Bulbine natalensis (Baker) stem on the sexual behaviour of male rats. Int J Androl 2009; 32: 629–636.
[19] Ratnasooriya WD, Dharmasiri MG. Effects of Terminalia catappa seeds on sexual behaviour and fertility of male rats. Asian J Androl 2000; 2: 213–219.
[20] Fouche G, Afolayan AJ, Wintola OA, Khorombi TE, Senabe J. Effect of the aqueous extract of the aerial parts of
Monsonia angustifolia E. Mey. Ex A. Rich., on the sexual behaviour of male Wistar rats. BMC Complement Altern
Med 2015; 15: 343.
[21] Kale OE, Awodele O, Akindele AJ. Acridocarpus smeathmannii (DC.) Guill. & Perr. Root enhanced reproductive behavior and sexual function in male wistar rats: Biochemical and pharmacological mechanisms. J Ethnopharmaco 2019; 230: 95–108.
[22] Andric SA, Janjic MM, Stojkov NJ, Kostic TS. Sildenafil treatment in vivo stimulates Leydig cell steroidogenesis
via the cAMP/cGMP signaling pathway. Am J Physiol Endocrinol Metab 2010; 299: E544–550.
[23] Besong EB, Ateufack G, Babiaka SB, Kamanyi A. Leafmethanolic extract of Pseudopanax arboreus (Araliaceae) (L. F. Phillipson) reverses amitriptyline-induced sexual dysfunction in male rats. Biochem Res Int 2018; 2018: 2869727.
[24] Zade V, Dabhadkar D, Thakare V, Pare S. Evaluation of potential aphrodisiac activity of Moringa oleifera seed in male albino rats. Int J Pharm Pharm Sci 2013; 5: 683–689.
[25] Gayrard V. Physiologie de la reproduction des mammifères. Ecole Nationale Vétérinaire, Toulouse 2007; 198.
[26] Gupta RS, Kachhawa JB, Chaudhary R. Antifertility effects of methanolic pod extract of Albizzia lebbeck (L.) Benth in male rats. Asian J Androl 2004; 6: 155–159.
[27] Chung HJ, Noh Y, Kim MS, Jang A, Lee CE, Myung SC. Steroidogenic effects of Taraxacum officinale extract on
the levels of steroidogenic enzymes in mouse Leydig cells. Anim Cells Syst (Seoul) 2018; 22: 407–414.
[28] Muniyan S, Chaturvedi NK, Dwyer JG, LaGrange CA, Chaney WG, Lin MF. Human prostatic acid phosphatase:
Structure, function and regulation. Int J Mol Sci 2013; 14: 10438–10464.
[29] Jian Feng C, Peng Ying Z, Cheng Wei X, Tao Tao H, Yun Gui B, Kao Shan C. Effect of aqueous extract of Arctium lappa L. (burdock) roots on the sexual behavior of male rats. BMC Complement Altern Med 2012; 12: 1–8.
[30] Hnatyszyn O, Moxatelli V, Rondina R, Costa M, Arranz C, Belaszczuk A, et al. Flavonoids from Achyzocline
satureiodes with relaxant effects on the smooth muscle in Guinea pig corpus cavernosum. Phytomedicine 2004; 11: 366–369.
[31] Kim HJ, Woo DS, Lee G, Kim JJ. The relaxation effects of ginseng saponin in rabbit corporal smooth muscle, is it a nitric oxide donor? Br J Urol 1998; 82: 744–748.