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Dorfeshan, P., Ghaffari Novin, M., Salehi, M., & Farifteh, F. (2019). Expression of miR-302 in human embryo derived from in-vitro matured oocyte. International Journal of Reproductive BioMedicine (IJRM), 17(6), 405–412. https://doi.org/10.18502/ijrm.v17i6.4812
https://doi.org/10.18502/ijrm.v17i6.4812
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- Cited by 2 articles
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Parvin Dorfeshan
Parvin Dorfeshan
Department of Social Medicine, Faculty of Medicine, Jundishapure University of Medical Sciences, Ahvaz, Iran; Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Marefat Ghaffari Novin
Marefat Ghaffari Novin
Infertility and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Mohammad Salehi
Mohammad Salehi
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Fatane Farifteh
Fatane Farifteh
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Published Date
- Jul 29, 2019
Expression of miR-302 in human embryo derived from in-vitro matured oocyte
Abstract
Background: The expression of miR-302 over the period of early embryogenesis could possibly regulate the maternal transcript clearance. Zygotic transcription activation is mostly related to maternal messages degradation.
Objective: In this study, the effects of in-vitro maturation technique (IVM) on the expression of miR-302 in human embryo produced from immature and mature human oocytes (matured in vitro and in vivo, before sperm exposure) obtained from females under gonadotrophin therapy were evaluated for assisted reproduction.
Materials and Methods: Immature oocytes were cultured in vitro. The injection of oocytes-producing polar bodies was given using fresh sperm. Then, the embryo quality score was assessed in the IVM group compared with the control group. In both the groups, embryos with normal morphology were included in the molecular study. Only one blastomere was removed from three-day embryos and then the embryos were frozen. The expression of miR-302 in embryos was measured through quantitative realtime polymerase chain reaction.
Results: Our data showed a significant reduction of miR-302 expression in the IVM group vs. the control group (p = 0.02). The embryo quality score showed a significant difference between the two groups (p = 0.01).
Conclusion: The present study demonstrated that the IVM process had a negative effect on the expression level of miR-302 in human pre-implantation embryos. Considering the major role of expression miR-302, a reduced potential in miR-302 expression could be related to a decrease in the early embryonic development.
References
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[14] Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, et al. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci USA 2009; 106: 15720–15725.
[15] Lin SL, Chang DC, Chang-Lin S, Lin CH, Wu DT, Chen DT, et al. Mir-302 reprograms human skin cancer cells into a pluripotent ES-cell-like state. RNA 2008; 14: 2115–2124.
[16] Chen PY, Manninga H, Slanchev K, Chien M, Russo JJ, Ju J, et al. The developmental miRNA profiles of zebrafish as determined by small RNA cloning. Genes Dev 2005; 19: 1288–1293.
[17] Giraldez AJ. microRNAs, the cell’s Nepenthe: clearing the past during the maternal-to-zygotic transition and cellular reprogramming. Curr Opin Genet Dev 2010; 20: 369–375.
[18] Melton C, Judson RL, Blelloch R. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 2010; 463: 621–626.
[19] Foshay KM, Gallicano GI. miR-17 family miRNAs are expressed during early mammalian development and
regulate stem cell differentiation. Dev Biol 2009; 326: 431– 443.
[20] Rosenbluth EM, Shelton DN, Sparks AE, Devor E, Christenson L, Van Voorhis BJ. MicroRNA expression in the human blastocyst. Fertil Steril 2013; 99: 855–861.
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[22] Kruger TF, Menkveld R, Stander FS, Lombard CJ, Van der Merwe JP, van Zyl JA, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 1986; 46: 1118–1123.
[23] Nasr-Esfahani MH, Salehi M, Razavi S, Mardani M, Bahramian H, Steger K, et al. Effect of protamine-2
deficiency on ICSI outcome. Reprod Biomed Online 2004; 9: 652–658.
[24] Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E. High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 2003; 80: 223–224.
[25] Dehghani-Mohammadabadi M, Salehi M, Farifteh F, Nematollahi S, Arefian E, Hajjarizadeh A, et al. Melatonin
modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice.
J Assist Reprod Genet 2014; 31: 453–461.
[26] Zuccotti M, Boiani M, Ponce R, Guizzardi S, Scandroglio R, Garagna S, et al. Mouse xist expression begins at zygotic genome activation and is timed by a zygotic clock. Mol Reprod Dev 2002; 61: 14–20.
[27] Haghpanah T, Salehi M, Ghaffari Novin M, Masteri Farahani R, Fadaei-Fathabadi F, Dehghani Mohammadabadi M, et al. Does sperm DNA fragmentation affect the developmental potential and the incidence of apoptosis following blastomere biopsy? Syst Biol Reprod Med 2016; 62: 1–10.
[28] Gilchrist GC, Tscherner A, Nalpathamkalam T, Merico D, LaMarre J. MicroRNA Expression during Bovine Oocyte Maturation and Fertilization. Int J Mol Sci 2016; 17: 396.
[29] Lin SL. Concise review: Deciphering the mechanism behind induced pluripotent stem cell generation. Stem
Cells 2011; 29: 1645–1649.
[30] Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, et al. Human embryonic stem cells express a unique set of
microRNAs. Dev Biol 2004; 270: 488–498.
[31] Houbaviy HB, Murray MF, Sharp PA. Embryonic stem cellspecific MicroRNAs. Dev Cell 2003; 5: 351–358.
[32] De Sousa PA, Caveney A, Westhusin ME, Watson AJ. Temporal patterns of embryonic gene expression and their dependence on oogenetic factors. Theriogenology 1998; 49: 115–128.
[33] De La Fuente R, Eppig JJ. Transcriptional activity of the mouse oocyte genome: companion granulosa cells
modulate transcription and chromatin remodeling. Dev Biol 2001; 229: 224–236.
[34] Gardner DK, Sheehan CB, Rienzi L, Katz-Jaffe M, Larman MG. Analysis of oocyte physiology to improve cryopreservation procedures. Theriogenology 2007; 67: 64–72.
[2] Chian RC, Buckett WM, Tan SL. In-vitro maturation of human oocytes. Reprod Biomed Online 2004; 8: 148–166.
[3] Lee MT, Bonneau AR, Giraldez AJ. Zygotic genome activation during the maternal-to-zygotic transition. Annu
Rev Cell Dev Biol 2014; 30: 581–613.
[4] Plath K, Lowry WE. Progress in understanding reprogramming to the induced pluripotent state. Nat Rev Genet 2011;12: 253–265.
[5] Lin SL, Chang DC, Lin CH, Ying SY, Leu D, Wu DT. Regulation of somatic cell reprogramming through inducible mir302 expression. Nucleic Acids Res 2010; 39: 1054–1065.
[6] Card DA, Hebbar PB, Li L, Trotter KW, Komatsu Y, Mishina Y, et al. Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells. Mol Cell Biol 2008; 28:6426–6438.
[7] Barroso-delJesus A, Lucena-Aguilar G, Sanchez L, Ligero G, Gutierrez-Aranda I, Menendez P. The nodal inhibitor lefty is negatively modulated by the microRNA miR-302 in human embryonic stem cells. FASEB J 2011; 25: 1497–1508.
[8] Heyn P, Kircher M, Dahl A, Kelso J, Tomancak P, Kalinka AT, et al. The earliest transcribed zygotic genes are short, newly evolved, and different across species. Cell Rep 2014; 6: 285–292.
[9] Lee MT, Bonneau AR, Takacs CM, Bazzini AA, DiVito KR, Fleming ES, et al. Nanog, Pou5f1 and SoxB1 activate
zygotic gene expression during the maternal-to-zygotic transition. Nature 2013; 503: 360–364.
[10] Bazzini AA, Lee MT, Giraldez AJ. Ribosome profiling shows that miR-430 reduces translation before causing
mRNA decay in zebrafish. Science 2012; 336: 233–237.
[11] Giraldez AJ, Mishima Y, Rihel J, Grocock RJ, Van Dongen S, Inoue K, et al. Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs. Science 2006; 312:75–79.
[12] Schoorlemmer J, Van Puijenbroek A, van Den Eijnden M, Jonk L, Pals C, Kruijer W. Characterization of a negative retinoic acid response element in the murine Oct4 promoter. Mol Cell Biol 1994; 14: 1122–1136.
[13] Subramanyam D, Lamouille S, Judson RL, Liu JY, Bucay N, Derynck R, et al. Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. Nat Biotechnol 2011; 29: 443–448.
[14] Sun N, Panetta NJ, Gupta DM, Wilson KD, Lee A, Jia F, et al. Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci USA 2009; 106: 15720–15725.
[15] Lin SL, Chang DC, Chang-Lin S, Lin CH, Wu DT, Chen DT, et al. Mir-302 reprograms human skin cancer cells into a pluripotent ES-cell-like state. RNA 2008; 14: 2115–2124.
[16] Chen PY, Manninga H, Slanchev K, Chien M, Russo JJ, Ju J, et al. The developmental miRNA profiles of zebrafish as determined by small RNA cloning. Genes Dev 2005; 19: 1288–1293.
[17] Giraldez AJ. microRNAs, the cell’s Nepenthe: clearing the past during the maternal-to-zygotic transition and cellular reprogramming. Curr Opin Genet Dev 2010; 20: 369–375.
[18] Melton C, Judson RL, Blelloch R. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 2010; 463: 621–626.
[19] Foshay KM, Gallicano GI. miR-17 family miRNAs are expressed during early mammalian development and
regulate stem cell differentiation. Dev Biol 2009; 326: 431– 443.
[20] Rosenbluth EM, Shelton DN, Sparks AE, Devor E, Christenson L, Van Voorhis BJ. MicroRNA expression in the human blastocyst. Fertil Steril 2013; 99: 855–861.
[21] World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th Ed.Switzerland: WHO Press; 2010.
[22] Kruger TF, Menkveld R, Stander FS, Lombard CJ, Van der Merwe JP, van Zyl JA, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 1986; 46: 1118–1123.
[23] Nasr-Esfahani MH, Salehi M, Razavi S, Mardani M, Bahramian H, Steger K, et al. Effect of protamine-2
deficiency on ICSI outcome. Reprod Biomed Online 2004; 9: 652–658.
[24] Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E. High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 2003; 80: 223–224.
[25] Dehghani-Mohammadabadi M, Salehi M, Farifteh F, Nematollahi S, Arefian E, Hajjarizadeh A, et al. Melatonin
modulates the expression of BCL-xl and improve the development of vitrified embryos obtained by IVF in mice.
J Assist Reprod Genet 2014; 31: 453–461.
[26] Zuccotti M, Boiani M, Ponce R, Guizzardi S, Scandroglio R, Garagna S, et al. Mouse xist expression begins at zygotic genome activation and is timed by a zygotic clock. Mol Reprod Dev 2002; 61: 14–20.
[27] Haghpanah T, Salehi M, Ghaffari Novin M, Masteri Farahani R, Fadaei-Fathabadi F, Dehghani Mohammadabadi M, et al. Does sperm DNA fragmentation affect the developmental potential and the incidence of apoptosis following blastomere biopsy? Syst Biol Reprod Med 2016; 62: 1–10.
[28] Gilchrist GC, Tscherner A, Nalpathamkalam T, Merico D, LaMarre J. MicroRNA Expression during Bovine Oocyte Maturation and Fertilization. Int J Mol Sci 2016; 17: 396.
[29] Lin SL. Concise review: Deciphering the mechanism behind induced pluripotent stem cell generation. Stem
Cells 2011; 29: 1645–1649.
[30] Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, et al. Human embryonic stem cells express a unique set of
microRNAs. Dev Biol 2004; 270: 488–498.
[31] Houbaviy HB, Murray MF, Sharp PA. Embryonic stem cellspecific MicroRNAs. Dev Cell 2003; 5: 351–358.
[32] De Sousa PA, Caveney A, Westhusin ME, Watson AJ. Temporal patterns of embryonic gene expression and their dependence on oogenetic factors. Theriogenology 1998; 49: 115–128.
[33] De La Fuente R, Eppig JJ. Transcriptional activity of the mouse oocyte genome: companion granulosa cells
modulate transcription and chromatin remodeling. Dev Biol 2001; 229: 224–236.
[34] Gardner DK, Sheehan CB, Rienzi L, Katz-Jaffe M, Larman MG. Analysis of oocyte physiology to improve cryopreservation procedures. Theriogenology 2007; 67: 64–72.