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Yousefalizadegan, N., Mousavi, Z., Rastegar, T., Razavi, Y., & Najafizadeh, P. (2019). Reproductive toxicity of manganese dioxide in forms of micro- and nanoparticles in male rats. International Journal of Reproductive BioMedicine (IJRM), 17(5), 361–370. https://doi.org/10.18502/ijrm.v17i5.4603
https://doi.org/10.18502/ijrm.v17i5.4603
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- Cited by 6 articles
authors
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Narges Yousefalizadegan
Narges Yousefalizadegan
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
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Zahra Mousavi
Zahra Mousavi
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
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Tayebeh Rastegar
Tayebeh Rastegar
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
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Yasaman Razavi
Yasaman Razavi
Cellular Molecular Research Center Iran University of Medical Sciences, Tehran, Iran.
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Parvaneh Najafizadeh
Parvaneh Najafizadeh
Department of Pharmacology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Published Date
- Jun 18, 2019
Reproductive toxicity of manganese dioxide in forms of micro- and nanoparticles in male rats
Abstract
Background: Manganese Dioxide (MnO2) has long been used in industry, and its application has recently been increasing in the form of nanoparticle.
Objective: The present study was an attempt to assess the effects of MnO2 nanoparticles on spermatogenesis in male rats.
Materials and Methods: Micro- and nanoparticles of MnO2 were injected (100 mg/kg) subcutaneously to male Wistar rats (150 ± 20 gr) once a week for a period of 4 weeks, and the vehicle group received only normal saline (each group included 8 rats). The effect of these particles on the bodyweight, number of sperms, spermatogonia, spermatocytes, diameter of seminiferous tubes, testosterone, estrogen, follicle stimulating factor, and the motility of sperms were evaluated and then compared among the control and vehicle groups as the criteria for spermatogenesis.
Results: The results showed that a chronic injection of MnO2 nanoparticles caused a significant decrease in the number of sperms, spermatogonia, spermatocytes, diameter of seminiferous tubes (p < 0.001) and in the motility of sperms. However, no significant difference was observed in the weight of prostate, epididymis, left testicle, estradiol (p = 0.8) and testosterone hormone (p = 0.2).
Conclusion: It seems that the high oxidative power of both particles was the main reason for the disturbances in the function of the testis. It is also concluded that these particles may have a potential reproductive toxicity in adult male rats. Further studies are thus needed to determine its mechanism of action upon spermatogenesis.
References
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Nanomedicine Journal 2014; 1: 180–190.
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[12] Zhang Y, Yang Y, Zhang Y, Zhang T, Ye M. Heterogeneous oxidation of naproxen in the presence of α-MnO2 nanostructures with different morphologies. Applied Catalysis B: Environmental 2012; 127: 182–189.
[13] Najafizadeh P, Dehghani F, Panjeh Shahin M, Hamzei Taj S. The effect of a hydro-alcoholic extract of olive fruit on reproductive argons in male Sprague-Dawley rat. Iran J Reprod Med 2013; 11: 293–300.
[14] Seed J, Chapin RE, Clegg ED, Dostal LA, Foote RH, Hurtt ME, et al. Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. ILSI risk science institute expert working group on sperm evaluation. Reprod Toxicol 1996; 10: 237– 244.
[15] Noorafshan A, Karbalay-Doust S. A simple method for unbiased estimating of ejaculated sperm tail length in subjects with normal and abnormal sperm motility. Micron 2010; 41: 96–99.
[16] Karbalay-Doust S, Noorafshan A, Dehghani F, Panjehshahin MR, Monabati A. Effects of hydroalcoholic extract of Matricaria chamomilla on serum testosterone and estradiol levels, spermatozoon quality, and tail length in rat. Iran J Med Sci 2010; 35: 122–128.
[17] Merry BJ. Molecular mechanisms linking calorie restriction and longevity. Int J Biochem Cell Biol 2002; 34: 1340–1354.
[18] Wang B, Feng WY, Wang TC, Jia G, Wang M, Shi JW, et al. Acute toxicity of nano-and micro-scale zinc powder in healthy adult mice. Toxicol Lett 2006; 161: 115–123.
[19] Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, et al. Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B 2008; 112: 13608–13619.
[20] Komatsu T, Tabata M, Kubo-Irie M, Shimizu T, Suzuki K, Nihei Y, et al. The effects of nanoparticles on mouse testis Leydig cells in vitro. Toxicol In Vitro 2008; 22: 1825–1831.
[21] Arakane F, King SR, Du Y, Kallen CB, Walsh LP, Watari H, et al. Phosphorylation of steroidogenic acute regulatory protein (StAR) modulates its steroidogenic activity. J Biol Chem 1997; 272: 32656–32662.
[22] Ajdary M, Ghahnavieh MZ, Naghsh N. Sub-chronic toxicity of gold nanoparticles in male mice. Adv Biomed Res 2015; 4: 67.
[23] Miresmaeili SM, Halvaei I, Fesahat F, Fallah A, Nikonahad N, Taherinejad M. Evaluating the role of silver nanoparticles on acrosomal reaction and spermatogenic cells in rat. Iran J Reprod Med 2013; 11: 423–430.
[24] Ema M, Kobayashi N, Naya M, Hanai S, Nakanishi J. Reproductive and developmental toxicity studies of manufactured nanomaterials. Reprod Toxicol 2010; 30: 343–352.
[25] Mohammadi Fartkhooni F, Noori A, Momayez M, Sadeghi L, Shirani K, Yousefi Babadi V. The effects of nano titanium dioxide (TiO2 ) in spermatogenesis in Wistar rat. Euro J Exp Bio 2013; 3: 145–149.
[26] Zakhidov ST, Pavlyuchenkova SM, Marshak TL, Rudoy VM, Dement’eva OV, Zelenina IA, et al. Effect of gold nanoparticles on mouse
spermatogenesis. Izv Akad Nauk Ser Biol 2012; 39: 279–287.
[27] Nazar M, Talebi AR, Hosseini Sharifabad M, Abbasi A, Khoradmehr A, Danafar AH. Acute and chronic effects of gold nanoparticles on sperm parameters and chromatin structure in Mice. Int J Reprod BioMed 2016; 14: 637–642.
[28] Sundarraj K, Manickam V, Raghunath A, Periyasamy M, Viswanathan MP, Perumal E. Repeated exposure to iron oxide nanoparticles causes testicular toxicity in mice. Environ Toxicol 2017; 32: 594–608.
[29] Kobayashi H, Larson K, Sharma RK, Nelson DR, Evenson DP, Toma H, et al. DNA damage in patients with untreated cancer as measured by the sperm chromatin structure assay. Fertil Steril 2001; 75: 469–475.
[30] Choi SM, Yoo SD, Lee BM. Toxicological characteristics of endocrine-disrupting chemicals: developmental toxicity, carcinogenicity, and mutagenicity. J Toxicol Environ Health B Crit Rev 2004; 7: 1–24.
[2] Kim T, Momin E, Choi J, Yuan K, Zaidi H, Kim J, et al. Mesoporous silica-coated hollow manganese oxide
nanoparticles as positive T 1 contrast agents for labeling and MRI tracking of adipose-derived mesenchymal stem cells. J Am Chem Soc 2011; 133: 2955–2961.
[3] Mousavi Z, Hassanpourezatti M, Najafizadeh P, Rezagholian S, Rhamanifar MS, Nosrati N. Effects of subcutaneous injection MnO2 micro-and nanoparticles on blood glucose level and lipid profile in rat. Iran J Med Sci 2016; 41: 518– 524.
[4] Mahmoudi M, Hofmann H, Rothen-Rutishauser B, PetriFink A. Assessing the in vitro and in vivo toxicity of
superparamagnetic iron oxide nanoparticles. Chem Rev 2011; 112: 2323–2338.
[5] Huang CC. Parkinsonism induced by chronic manganese intoxication-an experience in Taiwan. Chang Gung Med J 2007; 30: 385–395.
[6] Lan Z, Yang WX. Nanoparticles and spermatogenesis: how do nanoparticles affect spermatogenesis and penetrate the blood-testis barrier. Nanomedicine (Lond) 2012; 7:579–596.
[7] Talebi AR, Khorsandi L, Moridian M. The effect of zinc oxide nanoparticles on mouse spermatogenesis. J Assist
Reprod Genet 2013; 30: 1203–1209.
[8] Takeda K, Suzuki K-i, Ishihara A, Kubo-Irie M, Fujimoto R, Tabata M, et al. Nanoparticles transferred from pregnant mice to their offspring can damage the genital and cranial nerve systems. Journal of Health Science 2009; 55: 95– 102.
[9] Wiwanitkit V, Sereemaspun A, Rojanathanes R. Effect of gold nanoparticles on spermatozoa: the first world report. Fertil Steril 2009; 91: e7–e8.
[10] Nosrati N, Hassanpour-Ezzati M, Mousavi SZ, Rezagholiyan MS, Rezagholiyan Sh. Comparison of MnO2 nanoparticles and microparticles distribution in CNS and muscle and effect on acute pain threshold in rats.
Nanomedicine Journal 2014; 1: 180–190.
[11] Rezagolian S, Hassanpourezatti M, Mousavi SZ, Rhamanifar M, Nosrati N. Comparison of chronic administration of manganese oxide micro and nanoparticles on liver function parameters in male rats. Daneshvar Med 2013; 20 (Suppl.): 35–46.
[12] Zhang Y, Yang Y, Zhang Y, Zhang T, Ye M. Heterogeneous oxidation of naproxen in the presence of α-MnO2 nanostructures with different morphologies. Applied Catalysis B: Environmental 2012; 127: 182–189.
[13] Najafizadeh P, Dehghani F, Panjeh Shahin M, Hamzei Taj S. The effect of a hydro-alcoholic extract of olive fruit on reproductive argons in male Sprague-Dawley rat. Iran J Reprod Med 2013; 11: 293–300.
[14] Seed J, Chapin RE, Clegg ED, Dostal LA, Foote RH, Hurtt ME, et al. Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. ILSI risk science institute expert working group on sperm evaluation. Reprod Toxicol 1996; 10: 237– 244.
[15] Noorafshan A, Karbalay-Doust S. A simple method for unbiased estimating of ejaculated sperm tail length in subjects with normal and abnormal sperm motility. Micron 2010; 41: 96–99.
[16] Karbalay-Doust S, Noorafshan A, Dehghani F, Panjehshahin MR, Monabati A. Effects of hydroalcoholic extract of Matricaria chamomilla on serum testosterone and estradiol levels, spermatozoon quality, and tail length in rat. Iran J Med Sci 2010; 35: 122–128.
[17] Merry BJ. Molecular mechanisms linking calorie restriction and longevity. Int J Biochem Cell Biol 2002; 34: 1340–1354.
[18] Wang B, Feng WY, Wang TC, Jia G, Wang M, Shi JW, et al. Acute toxicity of nano-and micro-scale zinc powder in healthy adult mice. Toxicol Lett 2006; 161: 115–123.
[19] Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, et al. Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species. J Phys Chem B 2008; 112: 13608–13619.
[20] Komatsu T, Tabata M, Kubo-Irie M, Shimizu T, Suzuki K, Nihei Y, et al. The effects of nanoparticles on mouse testis Leydig cells in vitro. Toxicol In Vitro 2008; 22: 1825–1831.
[21] Arakane F, King SR, Du Y, Kallen CB, Walsh LP, Watari H, et al. Phosphorylation of steroidogenic acute regulatory protein (StAR) modulates its steroidogenic activity. J Biol Chem 1997; 272: 32656–32662.
[22] Ajdary M, Ghahnavieh MZ, Naghsh N. Sub-chronic toxicity of gold nanoparticles in male mice. Adv Biomed Res 2015; 4: 67.
[23] Miresmaeili SM, Halvaei I, Fesahat F, Fallah A, Nikonahad N, Taherinejad M. Evaluating the role of silver nanoparticles on acrosomal reaction and spermatogenic cells in rat. Iran J Reprod Med 2013; 11: 423–430.
[24] Ema M, Kobayashi N, Naya M, Hanai S, Nakanishi J. Reproductive and developmental toxicity studies of manufactured nanomaterials. Reprod Toxicol 2010; 30: 343–352.
[25] Mohammadi Fartkhooni F, Noori A, Momayez M, Sadeghi L, Shirani K, Yousefi Babadi V. The effects of nano titanium dioxide (TiO2 ) in spermatogenesis in Wistar rat. Euro J Exp Bio 2013; 3: 145–149.
[26] Zakhidov ST, Pavlyuchenkova SM, Marshak TL, Rudoy VM, Dement’eva OV, Zelenina IA, et al. Effect of gold nanoparticles on mouse
spermatogenesis. Izv Akad Nauk Ser Biol 2012; 39: 279–287.
[27] Nazar M, Talebi AR, Hosseini Sharifabad M, Abbasi A, Khoradmehr A, Danafar AH. Acute and chronic effects of gold nanoparticles on sperm parameters and chromatin structure in Mice. Int J Reprod BioMed 2016; 14: 637–642.
[28] Sundarraj K, Manickam V, Raghunath A, Periyasamy M, Viswanathan MP, Perumal E. Repeated exposure to iron oxide nanoparticles causes testicular toxicity in mice. Environ Toxicol 2017; 32: 594–608.
[29] Kobayashi H, Larson K, Sharma RK, Nelson DR, Evenson DP, Toma H, et al. DNA damage in patients with untreated cancer as measured by the sperm chromatin structure assay. Fertil Steril 2001; 75: 469–475.
[30] Choi SM, Yoo SD, Lee BM. Toxicological characteristics of endocrine-disrupting chemicals: developmental toxicity, carcinogenicity, and mutagenicity. J Toxicol Environ Health B Crit Rev 2004; 7: 1–24.