https://doi.org/10.18502/ijrm.v17i4.4553
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- Cited by 3 articles
authors
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Sepideh Sadeghi
Sepideh Sadeghi
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
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Ali Reza Talebi
Ali Reza Talebi
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
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Abbas Shahedi
Abbas Shahedi
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
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Mohammad Reza Moein
Mohammad Reza Moein
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
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Abolghasem Abbasi-sarcheshmeh
Abolghasem Abbasi-sarcheshmeh
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Published Date
- Jun 10, 2019
Effects of different doses of tamoxifen on the sperm parameters and chromatin quality in mice: An experimental model
Abstract
Background: Tamoxifen (TX) is widely used for the treatment of male factor and idiopathic infertility. It has been shown that TX induces sperm production and so improves male fertility.
Objective: This study evaluated the effects of different doses of TX on the sperm parameters and chromatin quality in mice.
Materials and Methods: In this research, 24 male NMRI mice were divided into three groups including group A: control animal receiving vehicle; group B: the group receiving basal diet and TX 0.4 mg/kg/day; and group C: the group receiving basal diet and TX 0.6 mg/kg/day for 35 days. Thereafter, epididymal spermatozoa were analyzed for standard parameters and nuclear chromatin quality using Aniline Blue (AB) and Toluidine Blue (TB) staining.
Results: The results indicated that although the TX did not affect the sperm count, motility, and viability parameters, it could elevate the percentage of sperm cells with abnormal morphology and abnormal chromatin at both doses. In addition, in comparison with the control mice, a significant elevation was observed in spermatozoa with residual histones (assessed by AB staining) at high doses of TX.
Conclusion: Our experimental data in mice suggested that the use of TX for treating male infertility might increase the rates of spermatozoa with abnormal chromatin in a dose-dependent manner.
References
[2] Saucedo M, Deneux-Tharaux C, Bouvier-Colle MH, French National Experts Committee on Maternal Mortality. Ten years of confidential inquiries into maternal deaths in France, 1998–2007. Obstet Gynecol 2013; 122: 752–760.
[3] Fatemeh T, Marziyeh G, Nayereh G, Anahita G, Samira T. Maternal and perinatal outcome in nulliparious women complicated with pregnancy hypertension. J Pak Med Assoc 2010; 60: 707–710.
[4] Creanga AA, Syverson C, Seed K, Callaghan WM. Pregnancy-related mortality in the United States, 2011–2013. Obstet Gynecol 2017; 130: 366–373.
[5] Walker JJ. Pre-eclampsia. Lancet 2000; 356: 1260–1265.
[6] Novelli GP, Valensise H, Vasapollo B, Larciprete G, Di Pierro G, Altomare F, et al. Are gestational and essential
hypertension similar? Left ventricular geometry and diastolic function. Hypertens Pregnancy 2003; 22: 225–237.
[7] Simmons LA, Gillin AG, Jeremy RW. Structural and functional changes in left ventricle during normotensive and preeclamptic pregnancy. Am J Physiol Heart Circ Physiol 2002; 283: H1627–H1633.
[8] Sibai BM, Habli M. Hypertensin disease of pregnancy. In: Gibbs R, Karlan BY, Hanay SF, Nygard I. Danforth’
Sobsetetrics & Gynecology. 10th ed. Lippencat & Willksins; USA: 2008. p.258–359.
[9] James SK, Lindahl B, Siegbahn A, Stridsberg M, Venge P, Armstrong P, et al. N-terminal pro-brain natriuretic peptide and other risk markers for the separate prediction of mortality and subsequent myocardial infarction in patients with unstable coronary artery disease: a global utilization of strategies to open occluded arteries (GUSTO)-IV substudy. Circulation 2003; 108: 275–281.
[10] Fazlinezhad A, Rezaeian MK, Yousefzadeh H, Ghaffarzadegan K, Khajedaluee M. Plasma brain natriuretic
peptide (BNP) as an indicator of left ventricular function, early outcome and mechanical complications after acute myocardial infarction. Clin Med Insights Cardiol 2011; 5:77–83.
[11] Afshani N, Moustaqim-Barrette A, Biccard BM, Rodseth RN, Dyer RA. Utility of B-type natriuretic peptides in
preeclampsia: a systematic review. Int J Obstet Anesth 2013; 22: 96–103.
[12] Raflic Hamad R, Larsson A, Pernow J, Bremme K, Eriksson MJ. Assessment of left ventricular structure and function in preeclapsia by echocardiography and cardiovascular biomarkers. J Hypertens 2009; 27: 2257–2264.
[13] Rafik Hamad R, Larsson A, Pernow J, Bremme K, Eriksson MJ. Assessment of left ventricular structure and function in preeclampsia by echocardiography and cardiovascular biomarkers. J Hypertension 2009; 27: 2257–2264.
[14] American college of obstetricians and gynecologists; task force on hypertension in pregnancy. Hypertension in pregnancy. Report of the american college of obstetricians and gynecologists’ task force on hypertension in pregnancy. Obstet Gynecol 2013; 122: 1122–1131.
[15] Resnik JL, Hong C, Resnik R, Kazanegra R, Beede J, Bhalla V, et al. Evaluation of B-type natriuretic peptide (BNP) levels in normal and preeclamptic women. Am J Obstet Gynecol 2005; 193: 450–454.
[16] Bakacak M, Serin S, Ercan O, Köstü B, Bakacak Z, Kiran H. Association of serum N-terminal pro-brain natriuretic peptide levels with the severity of preeclampsia. J Maternal Fetal Neonat Med 2016; 29: 2802–2806.
[17] Fayers S, Moodley J, Naidoo DP. Cardiovascular haemodynamics in pre-eclampsia using brain naturetic peptide and tissue Doppler studies. Cardiovasc J Afr 2013; 24: 130–136.