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Rahimi Anbarkeh, F., Baradaran, R., Ghandy, N., Jalali, M., Reza Nikravesh, M., & Soukhtanloo, M. (2019). Effects of monosodium glutamate on apoptosis of germ cells in testicular tissue of adult rat: An experimental study. International Journal of Reproductive BioMedicine (IJRM), 17(4), 261–270. https://doi.org/10.18502/ijrm.v17i4.4551
https://doi.org/10.18502/ijrm.v17i4.4551
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- Cited by 5 articles
authors
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Fatemeh Rahimi Anbarkeh
Fatemeh Rahimi Anbarkeh
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
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Raheleh Baradaran
Raheleh Baradaran
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
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Nasibeh Ghandy
Nasibeh Ghandy
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
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Mehdi Jalali
Mehdi Jalali
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
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Mohammad Reza Nikravesh
Mohammad Reza Nikravesh
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
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Mohammad Soukhtanloo
Mohammad Soukhtanloo
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Published Date
- Jun 10, 2019
Effects of monosodium glutamate on apoptosis of germ cells in testicular tissue of adult rat: An experimental study
Abstract
Background: Monosodium glutamate (MSG) is used as a flavoring and food seasoning. Some studies have reported the oxidative effects of using this substance on various tissues.
Objective: This study has investigated the effects of MSG and the protective effect of vitamin C (vit C) on apoptosis of testicular germ cells and biochemical factors.
Materials and Methods: In this experimental study, 24 adult male Wistar rats were randomly divided into four groups: control (received distilled water), vit C group (150 mg/kg), experimental group 1 (MSG 3 gr/kg), experimental group 2 (MSG 3 gr/kg + vit C 150 mg/kg). The rats were gavaged for 30 days, and then were sacrificed, the right testis was isolated for biochemical examinations for the glutathione, malondialdehyde, and left testis used in histological experiments. Tunnel staining was used to determine the number of apoptotic cells.
Results: The results showed that apoptotic cells in the MSG group had a significant increase compared to the control group (P = 0.001), but the number of these cells in the MSG co-administered with vit C and vit C groups were significantly lower than the MSG group. Germinal epithelial thickness also decreased in MSG group compared to the control group.
Conclusion: MSG can lead to increase apoptotic changes in the germinal epithelial of the testicle, and vit C as an antioxidant can modify the pathological and biochemical changes induced by MSG.
References
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[2] Hamza RZ, AL-Harbi MS. Monosodium glutamate induced testicular toxicity and the possible ameliorative role of vitamin E or selenium in male rats. Toxicol Rep 2014; 1: 1037–1045.
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[4] Ahluwalia P, Tewari K, Choudhary P. Studies on the effects of monosodium glutamate (MSG) on oxidative stress in erythrocytes of adult male mice. Toxicol Lett 1996; 84: 161– 165.
[5] El-Ezaby MM, Abd-El Hamide N-AH, El-Maksoud MAE, Shaheen EM, Embashi MMR. Effect of some food additives on lipid profile, kidney function and liver function of adult male albino rats. J Bas Environ Sci 2018; 5: 52–59.
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[8] Eweka A, Om’Iniabohs F. Histological studies of the effects of monosodium glutamate on the ovaries of adult wistar rats. Ann Med Health Sci Res 2011; 1: 37–43.
[9] Abdollahzadeh A, Kianifard D, Vafaei Saiah G. Study of the long-term and dose dependent effects of methylphenidate and monosodium glutamate on the hormonal alterations of the pituitary-testicular axis and sperm analysis in adolescence rats. Bulletin UASVM Veterinary Medicine 2017; 74:75–81.
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[11] Husarova V, Ostatnikova D. Monosodium glutamate toxic effects and their implications for human intake: a review. JMED Res 2013; 2013: 1–12.
[12] Iamsaard S, Sukhorum W, Samrid R, Yimdee J, Kanla P, Chaisiwamongkol K, et al. The sensitivity of male rat
reproductive organs to monosodium glutamate. Acta Med Acad 2014; 43: 3–9.
[13] Sargazi Z, Nikravesh MR, Jalali M, Sadeghnia HR, Rahimi Anbarkeh F. Apoptotic effect of organophosphorus insecticide diazinon on rat ovary and protective effect of vitamin E. Iran J Toxicol 2016; 10: 37–44.
[14] Rahimi Anbarkeh F, Nikravesh MR, Jalali M, Sadeghnia HR, Sargazi Z, Mohammdzadeh L. Single dose effect of
diazinon on biochemical parameters in testis tissue of adult rats and the protective effect of vitamin E. Iran J
Reprod BioMed 2014; 12: 731–736.
[15] Hosseini M, Anaeigoudari A, Beheshti F, Soukhtanloo M, Nosratabadi R. Protective effect against brain tissues oxidative damage as a possible mechanism for beneficial effects of L-arginine on lipopolysaccharide induced memory impairment in rats. Drug Chem Toxicol 2018; 41: 175–181.
[16] Kondoh T, Torii K. MSG intake suppresses weight gain, fat deposition, and plasma leptin levels in male Sprague– Dawley rats. Physiol Behav 2008; 95: 135–144.
[17] Ochiogu IS, Ogwu D, Uchendu CN, Okoye CN, Ihedioha JI, Mbegbu EC. Effects of monosodium-L-glutamate administration on serum levels of reproductive hormones and cholesterol, epididymal sperm reserves and testicular histomorphology of male albino rats. Acta Vet Hung 2015;63: 125–139.
[18] Ekaluo UB, Ikpeme EV, Ibiang YB, Amaechina OS. Attenuating role of vitamin C on sperm toxicity induced by
monosodium glutamate in albino rats. J Biol Sci 2013; 13: 298–301.
[19] Sakr SA, Badawy GM. Protective effect of curcumin on monosodium glutamate-induced reproductive toxicity in male albino rats. Global J Pharmacol 2013; 7: 416–422.
[20] Li HM, Gao X, Yang ML, Mei JJ, Zhang LT, Qiu XF. Effects of zuogui wan on neurocyte apoptosis and down-regulation of TGF- β expression in nuclei of arcuate hypothalamus of monosodium glutamate-liver regeneration rats. World J roenterol 2004; 10: 2823–2826.
[21] Pavlovic V, Cekic S, Sokolovic D, Djindjic B. Modulatory effect of monosodium glutamate on rat thymocyte proliferation and apoptosis. Bratisl Lek Listy 2006; 107: 185–191.
[22] Storto M, Sallese M, Salvatore L, Poulet R, Condorelli DF, Dell’Albani P, et al. Expression of metabotropic glutamate receptors in the rat and human testis. J Endocrinol 2001; 170: 71–78.
[23] Pavlovic V, Cekic S, Kocic G, Sokolovic D, Zivkovic V. Effect of monosodium glutamate on apoptosis and Bcl-2/Bax protein level in rat thymocyte culture. Physiol Res 2007; 56: 619–626.
[24] Miglio G, Varsaldi F, Dianzani C, Fantozzi R, Lombardi G. Stimulation of group I metabotropic glutamate receptors evokes calcium signals and c-jun and c-fos gene expression in human T cells. biochem pharmacol 2005; 70: 189–199.
[25] Alalwani AD. Monosodium glutamate induced testicular lesions in rats (histological study). Middle East Fertil Soc J 2014; 19: 274–280.
[26] Meier P, Finch A, Evan G. Apoptosis in development. Nature 2000; 407: 796–801.
[27] Rajaei F, Karja NW, Agung B, Wongsrikeao P, Taniguchi M, Murakami M, et al. Analysis of DNA fragmentation
of porcine embryos exposed to cryoprotectants. Reprod Domest Anim 2005; 40: 429–432.
[28] Pavlovic V, Pavlovic D, Kocic G, Sokolovic D, Sarac M, Jovic Z. Ascorbic acid modulates monosodium glutamate induced cytotoxicity in rat thymus. Bratisl Lek Listy 2009; 110: 205–209.
[29] Nayanatara A, Vinodini N, Damodar G, Ahemed B, Ramaswamy C, Shabarinath M, et al. Role of ascorbic acid
in monosodium glutamate mediated effect on testicular weight, sperm morphology and sperm count in rat testis. J Chin Clin Med 2008; 3: 1–5.
[2] Hamza RZ, AL-Harbi MS. Monosodium glutamate induced testicular toxicity and the possible ameliorative role of vitamin E or selenium in male rats. Toxicol Rep 2014; 1: 1037–1045.
[3] Henry-Unaeze HN. Update on food safety of monosodium l-glutamate (MSG). Pathophysiology 2017; 24: 243–249.
[4] Ahluwalia P, Tewari K, Choudhary P. Studies on the effects of monosodium glutamate (MSG) on oxidative stress in erythrocytes of adult male mice. Toxicol Lett 1996; 84: 161– 165.
[5] El-Ezaby MM, Abd-El Hamide N-AH, El-Maksoud MAE, Shaheen EM, Embashi MMR. Effect of some food additives on lipid profile, kidney function and liver function of adult male albino rats. J Bas Environ Sci 2018; 5: 52–59.
[6] Sharma A. Monosodium glutamate-induced oxidative kidney damage and possible mechanisms: a mini-review. J Biomed Sci 2015; 22: 93.
[7] Helal EGE, El-Sayed RAA, El-Gamal MS. Assessment of the Physiological Changes Induced by Sodium Nitrite, Annatto or Mono Sodium Glutamate in Male Albino Rats. EgyptianJ Hospital Med 2017; 67: 330–335.
[8] Eweka A, Om’Iniabohs F. Histological studies of the effects of monosodium glutamate on the ovaries of adult wistar rats. Ann Med Health Sci Res 2011; 1: 37–43.
[9] Abdollahzadeh A, Kianifard D, Vafaei Saiah G. Study of the long-term and dose dependent effects of methylphenidate and monosodium glutamate on the hormonal alterations of the pituitary-testicular axis and sperm analysis in adolescence rats. Bulletin UASVM Veterinary Medicine 2017; 74:75–81.
[10] Oforofuo IAO, Onakewhor JUE, Idaewor PE. The effect of chronic administration of MSG on the histology of the adult Wistar rat testes. Bio Res Comm 1997; 9: 30–56.
[11] Husarova V, Ostatnikova D. Monosodium glutamate toxic effects and their implications for human intake: a review. JMED Res 2013; 2013: 1–12.
[12] Iamsaard S, Sukhorum W, Samrid R, Yimdee J, Kanla P, Chaisiwamongkol K, et al. The sensitivity of male rat
reproductive organs to monosodium glutamate. Acta Med Acad 2014; 43: 3–9.
[13] Sargazi Z, Nikravesh MR, Jalali M, Sadeghnia HR, Rahimi Anbarkeh F. Apoptotic effect of organophosphorus insecticide diazinon on rat ovary and protective effect of vitamin E. Iran J Toxicol 2016; 10: 37–44.
[14] Rahimi Anbarkeh F, Nikravesh MR, Jalali M, Sadeghnia HR, Sargazi Z, Mohammdzadeh L. Single dose effect of
diazinon on biochemical parameters in testis tissue of adult rats and the protective effect of vitamin E. Iran J
Reprod BioMed 2014; 12: 731–736.
[15] Hosseini M, Anaeigoudari A, Beheshti F, Soukhtanloo M, Nosratabadi R. Protective effect against brain tissues oxidative damage as a possible mechanism for beneficial effects of L-arginine on lipopolysaccharide induced memory impairment in rats. Drug Chem Toxicol 2018; 41: 175–181.
[16] Kondoh T, Torii K. MSG intake suppresses weight gain, fat deposition, and plasma leptin levels in male Sprague– Dawley rats. Physiol Behav 2008; 95: 135–144.
[17] Ochiogu IS, Ogwu D, Uchendu CN, Okoye CN, Ihedioha JI, Mbegbu EC. Effects of monosodium-L-glutamate administration on serum levels of reproductive hormones and cholesterol, epididymal sperm reserves and testicular histomorphology of male albino rats. Acta Vet Hung 2015;63: 125–139.
[18] Ekaluo UB, Ikpeme EV, Ibiang YB, Amaechina OS. Attenuating role of vitamin C on sperm toxicity induced by
monosodium glutamate in albino rats. J Biol Sci 2013; 13: 298–301.
[19] Sakr SA, Badawy GM. Protective effect of curcumin on monosodium glutamate-induced reproductive toxicity in male albino rats. Global J Pharmacol 2013; 7: 416–422.
[20] Li HM, Gao X, Yang ML, Mei JJ, Zhang LT, Qiu XF. Effects of zuogui wan on neurocyte apoptosis and down-regulation of TGF- β expression in nuclei of arcuate hypothalamus of monosodium glutamate-liver regeneration rats. World J roenterol 2004; 10: 2823–2826.
[21] Pavlovic V, Cekic S, Sokolovic D, Djindjic B. Modulatory effect of monosodium glutamate on rat thymocyte proliferation and apoptosis. Bratisl Lek Listy 2006; 107: 185–191.
[22] Storto M, Sallese M, Salvatore L, Poulet R, Condorelli DF, Dell’Albani P, et al. Expression of metabotropic glutamate receptors in the rat and human testis. J Endocrinol 2001; 170: 71–78.
[23] Pavlovic V, Cekic S, Kocic G, Sokolovic D, Zivkovic V. Effect of monosodium glutamate on apoptosis and Bcl-2/Bax protein level in rat thymocyte culture. Physiol Res 2007; 56: 619–626.
[24] Miglio G, Varsaldi F, Dianzani C, Fantozzi R, Lombardi G. Stimulation of group I metabotropic glutamate receptors evokes calcium signals and c-jun and c-fos gene expression in human T cells. biochem pharmacol 2005; 70: 189–199.
[25] Alalwani AD. Monosodium glutamate induced testicular lesions in rats (histological study). Middle East Fertil Soc J 2014; 19: 274–280.
[26] Meier P, Finch A, Evan G. Apoptosis in development. Nature 2000; 407: 796–801.
[27] Rajaei F, Karja NW, Agung B, Wongsrikeao P, Taniguchi M, Murakami M, et al. Analysis of DNA fragmentation
of porcine embryos exposed to cryoprotectants. Reprod Domest Anim 2005; 40: 429–432.
[28] Pavlovic V, Pavlovic D, Kocic G, Sokolovic D, Sarac M, Jovic Z. Ascorbic acid modulates monosodium glutamate induced cytotoxicity in rat thymus. Bratisl Lek Listy 2009; 110: 205–209.
[29] Nayanatara A, Vinodini N, Damodar G, Ahemed B, Ramaswamy C, Shabarinath M, et al. Role of ascorbic acid
in monosodium glutamate mediated effect on testicular weight, sperm morphology and sperm count in rat testis. J Chin Clin Med 2008; 3: 1–5.