Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice


Background: Generation of free radicals and oxidative stress are a major contributor

to diabetes. These factors lead to the development of diabetic testicles disorders.

Objective: In this study, the protective effect of vitamin E on functional disorders

associated with diabetes induced oxidative stress in male reproductive systems has

been investigated.

Materials and Methods: Thirty-three adult male Mice were divided into control,

diabetic, and untreated diabetic groups. Streptozotocin was used to induce diabetes.

In the treated group, vitamin E was given to the Mice intraperitoneally for 30 days.

Then, animals were anesthetized and sacrificed. Animal testicles were isolated and

homogenized in phosphate buffer and used for measuring sperm count, motility and

survival of sperm, MDA concentration and antioxidant capacity (TAC). Apoptosis was

also performed with the TUNEL test.

Results: The results of reduction (12.03±98.11) TAC, MDA concentration (–28.5±2.58),

sperm motility (unstable sperma= 86.4±7.48), sperm count (171.51), Sperm morphology

(natural morphology= 49.69±31.93) and abnormal morphology (9.77±49.7)

with increased oxidative damage. These changes were statistically significant in

comparison with the control group for all variables other than MDA (p= 0.05). Treatment

of vitamin E diabetic Mice improved the ability of antioxidants to prevent oxidative

damage in the testicles, restore the sperm movement, and increase the number of

normal sperm as well as TAC. The level of apoptosis in the treated group has decreased

compared to the untreated group.

Conclusion: Vitamin E protects the reproductive system against diabetes mellitus.

Therefore, it was concluded that vitamin E may be a suitable agent for protecting the

sperm and testicular parameters against undesirable effects of diabetes.