Download fulltext HTML
Hosseini, S. M., Moshrefi, A. H., Amani, R., Razavimehr, S. V., Aghajanikhah2M. H., Sokouti, Z., & Holari, B. B. (2019). Subchronic effects of different doses of Zinc oxide nanoparticle on reproductive organs of female rats: An experimental study. International Journal of Reproductive BioMedicine (IJRM), 17(2), 107–118. https://doi.org/10.18502/ijrm.v17i2.3988

https://doi.org/10.18502/ijrm.v17i2.3988

statistics

  • 265 Abstract Views
  • 90 PDF Views
  • 0 HTML Views
  • Cited by 16 articles

authors

  • Seyed Mohammad Hosseini
    No author data available.
  • Amir Hossein Moshrefi
    No author data available.
  • Reza Amani
    No author data available.
  • Seyed Vahid Razavimehr
    No author data available.
  • Mohammad Hasan Aghajanikhah2
    No author data available.
  • Zahra Sokouti
    No author data available.
  • Behnam Babaei Holari
    No author data available.

Published Date

  • Mar 20, 2019

Subchronic effects of different doses of Zinc oxide nanoparticle on reproductive organs of female rats: An experimental study

International Journal of Reproductive BioMedicine (IJRM) / International Journal of Reproductive BioMedicine (IJRM): Volume 17, Issue No. 2 / Pages 107–118

Abstract

Background: Zinc performs many biochemical and physiological functions; however, toxicological studies demonstrate that Nano-zinc oxide has harmful effects on human health and environmental species in high concentrations.


Objective: The aim of this study was to investigate the toxicity of zinc oxide nanoparticles on reproductive tissues of female rat.


Materials and Methods: Eighty female Wistar adult rats weighing 180–200 gr, divided into eight groups (n= 10 in each group) including control, sham (treated with saline), and six groups injected with different doses of zinc oxide nanoparticle with 10–30 nanometer size (4, 8, 25, 50, 100, and 200 mg/kg) twice a week for four weeks. At the end of the study, the rats were bled and slaughtered; the Ovary and Uterus were taken for histopathology studies and blood samples were transferred to the laboratory for biochemical analysis.


Results: Microscopic diagnoses in ovary tissue were included; increase in the corpus luteum, follicular cysts, inflammatory cells infiltration and fibrosis. Histopathological changes in ovary in a dose-dependent manner. In uterus tissue the lesions consisted; epithelial destruction, hyperplasia of endometrial glands. The Estrogen and Progesterone level in the serum of rats increased in low doses and reduced in a dose-dependent manner at high doses.


Conclusion: The results of the current study proved the toxicity of zinc oxide nanoparticles on the ovary and uterus organs at high concentrations, so further investigation is needed to reduce these effects.


Key words: Zinc oxide, Nanoparticles, Ovary, Uterus, Toxicity.

References
[1] Duruibe JO, Ogwuegbu MOc, Egwurugwu J. Heavy metal pollution and human biotoxic effects. Int J Phys Sci 2007;2: 112–118.
[2] Wang ZL. Zinc oxide nanostructures: growth, properties and applications. J Phys Condens Matter 2004; 16: R829– R858.

[3] Khlifi R, Hamza-Chaffai A. Head and neck cancer due to heavy metal exposure via tobacco smoking and professional exposure: a review. Toxicol Appl Pharmacol 2010; 248: 71–88.

[4] Xia T, Kovochich M, Liong M, Mädler L, Gilbert B, Shi H, et al. Comparison of the mechanism of toxicity of
zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano
2008; 2: 2121–2134.

[5] Jaishankar M, Mathew BB, Shah MS, Murthy TPK, Gowda KRS. Biosorption of few heavy metal ions using agricultural wastes. J Environ Pollution Hum Health 2014; 2: 1–6.

[6] Haouas Z, Zidi I, Sallem A, Bhouri R, Ajina T, Zaouali M, et al. Reproductive toxicity of lead acetate in adult male rats: Histopathological and cytotoxic studies. J Cytol Histol 2015; 6: 293–299.

[7] Yamashita K, Yoshioka Y, Higashisaka K, Mimura K, Morishita Y, Nozaki M, et al. Silica and titanium dioxide
nanoparticles cause pregnancy complications in mice. Nat Nanotechnol 2011; 6: 321–328.

[8] Jo E, Seo G, Kwon JT, Lee M, Lee BC, Eom I, et al. Exposure to zinc oxide nanoparticles affects reproductive
development and biodistribution in offspring rats. J Toxicol Sci 2013; 38: 525–530.

[9] Gerhard I, Monga B, Waldbrenner A, Runnebaum B. Heavy metals and fertility. J Toxicol Environ Health A 1998; 54:593–611.

[10] Watanabe T, Shimada T, Endo A. Effects of mercury compounds on ovulation and meiotic and mitotic chromosomes in female golden hamsters. Teratology 1982; 25:381–384.

[11] Nohynek GJ, Lademann J, Ribaud C, Roberts MS. Grey goo on the skin? Nanotechnology, cosmetic and sunscreen safety. Crit Rev Toxicol 2007; 37: 251–277.

[12] Esmaeillou M, Moharamnejad M, Hsankhani R, Tehrani AA, Maadi H. Toxicity of ZnO nanoparticles in healthy adult mice. Environ Toxicol Pharmacol 2013; 35: 67–71.

[13] Mehta M, Hundal SS. Effect of sodium arsenite on reproductive organs of female Wistar rats. Arch Environ Occup Health 2016; 71: 16–25.

[14] Bires J, Maracek I, Bartko P, Biresova M, Weissova T. Accumulation of trace elements in sheep and the effects
upon qualitative and quantitative ovarian changes. Vet Hum Toxicol 1995; 37: 349–356.

[15] Chattopadhyay S, Pal Ghosh S, Ghosh D, Debnath J. Effect of dietary co-administration of sodium selenite on sodium arsenite-induced ovarian and uterine disorders in mature albino rats. Toxicol Sci 2003; 75: 412–422.

[16] Kong L, Tang M, Zhang T, Wang D, Hu K, Lu W, et al. Nickel nanoparticles exposure and reproductive toxicity in healthy adult rats. Int J Mol Sci 2014; 15: 21253–21269.

[17] Flora SJ, Mittal M, Mehta A. Heavy metal induced oxidative stress and its possible reversal by chelation therapy. Indian J Med Res 2008; 128: 501–523.

[18] Nampoothiri LP, Agarwal A, Gupta S. Effect of co-exposure to lead and cadmium on antioxidant status in rat ovarian granulose cells. Arch Toxicol 2007; 81: 145–150.

[19] Suhartono E, Amin Setyo Leksono T, Sasmito Djati M. Oxidative stress and kidney glycation in rats exposed
cadmium. Int J Chem Eng Appl 2014; 5: 497–501.

[20] Aflanie I, Muhyi R, Suhartono E. Effect of heavy metal on malondialdehyde and advanced oxidation protein produtcs concentration: A focus on arsenic, cadmium, and mercury. J Med BiolEng 2015; 4: 332–337.

[21] Shaheen AA, el-Fattah AA. Effect of dietary zinc on lipid peroxidation, glutathione, protein thiols levels and
superoxide dismutase activity in rat tissues. Int J Biochem Cell Biol 1995; 27: 89–95.

[22] da Silva Faria T, de Bittencourt Brasil F, Sampaio FJ, da Fonte Ramos C. Effects of maternal undernutrition during lactation on estrogen and androgen receptor expressions in rat ovary at puberty. Nutrition 2010; 26: 993–999.

[23] Drummond AE, Findlay JK. The role of estrogen in folliculogenesis. Mol Cell Endocrinol 1999; 151: 57–64.

[24] Kumar TR, Wang Y, Lu N, Matzuk MM. Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nat Genet 1997; 15: 201–204.

[25] Wang N, She Y, Zhu Y, Zhao H, Shao B, Sun H, et al. Effects of subchronic aluminum exposure on the reproductive function in female rats. Biol Trace Elem Res 2012; 145:382–387.

[26] Fazilati M. Investigation toxicity properties of zinc oxide nanoparticles on liver enzymes in male rat. Euro J Exp Bio 2013; 3: 104–109.