The protective effects of Crocin on testopathy in fat-fed and streptozotocin-treated diabetic rats: An experimental study


Background: Male hypogonadism is associated with type II diabetes mellitus due to

testicular dysfunction. Medicinal plants have received considerable attention for the

management of diabetes and its complications.

Objective: The aim of present study was to evaluate the anti-diabetic and protective

influence of Crocin on testopathy in diabetic rats.

Materials and Methods: In this experimental study, type II of diabetes mellitus was

induced by high-fat diet and low dose of streptozotocin. Male Wistar rats (8 weeks,

150–200 gr, 18 rats; n= 6 per group) were divided into a control group (standard

diet), diabetic group (streptozotocin+high-fat diet), and treatment group (High-fat

diet+streptozotocin+Crocin at 20 mg/kg/day, i.p. for 60 days). After 60 days, animals

were euthanized, testis and epididymis were dissected, and weights of testes and

sperm count were analyzed. Hematoxylin-eosin-stained was done for histopathological

examination. Blood samples were collected for the assessment of serum glucose and


Results: High-fat diet and streptozotocin significantly increased the serum glucose

and cholesterol levels as compared to the control group (p≤ 0. 001). Moreover, there

was a significant decrease in the weight of right (p= 0.008) and left testes (p≤ 0. 001)

and also the total sperm count (p= 0.023) in the diabetic group compared with the