Mohammad Reza Nikravesh
Hamid Reza Sadeghnia
Fatemeh Rahimi Anbarkeh
follicles following an administration of diazinon.
Materials and Methods: A total of 30 adult female Wistar rats were divided into five
groups: a control group (without any intervention), sham group (received only pure
olive oil, as solvent), experimental group I (DZN+olive oil, 60 mg/kg), experimental
group II (vitamin E, 200 mg/kg), and experimental group III (DZN: 60 mg/kg+vitamin
E: 200 mg/kg). All drugs were injected intraperitoneally, except vitamin E which was
administrated by gavage. The animals were scarified after two weeks and left ovary was
used to measure proliferation of ovarian follicles. Tissues were analyzed by the PCNA
technique and viewed with an optical microscope for evaluating cells proliferation.
Results: The result of the present study revealed that the number of proliferative cells
in the experimental group I decreased significantly in contrast to the control group
in secondary and Graffian follicles (p< 0.001). The administration of vitamin E plus
DZN significantly increased proliferative cells compared to the DZN group (p< 0.001).
Primordial follicles showed that all study groups were lacking PCNA positive cells,
which means no expression of PCNA in these follicles. The results of this study showed
that primary follicles in all study groups had a few and scattered PCNA positive cells
with no significant difference between the groups (p> 0.05).
Conclusion: Results showed that DZN reduced proliferation in secondary and Graffian
follicles and vitamin E increased it. The results of this study suggested that vitamin E
by its antioxidant activity was able to improve the DZN-induced ovarian toxicity.