The protective effect of vitamin E on rats’ ovarian follicles following an administration of diazinon: An experimental study


follicles following an administration of diazinon.

Materials and Methods: A total of 30 adult female Wistar rats were divided into five

groups: a control group (without any intervention), sham group (received only pure

olive oil, as solvent), experimental group I (DZN+olive oil, 60 mg/kg), experimental

group II (vitamin E, 200 mg/kg), and experimental group III (DZN: 60 mg/kg+vitamin

E: 200 mg/kg). All drugs were injected intraperitoneally, except vitamin E which was

administrated by gavage. The animals were scarified after two weeks and left ovary was

used to measure proliferation of ovarian follicles. Tissues were analyzed by the PCNA

technique and viewed with an optical microscope for evaluating cells proliferation.

Results: The result of the present study revealed that the number of proliferative cells

in the experimental group I decreased significantly in contrast to the control group

in secondary and Graffian follicles (p< 0.001). The administration of vitamin E plus

DZN significantly increased proliferative cells compared to the DZN group (p< 0.001).

Primordial follicles showed that all study groups were lacking PCNA positive cells,

which means no expression of PCNA in these follicles. The results of this study showed

that primary follicles in all study groups had a few and scattered PCNA positive cells

with no significant difference between the groups (p> 0.05).

Conclusion: Results showed that DZN reduced proliferation in secondary and Graffian

follicles and vitamin E increased it. The results of this study suggested that vitamin E

by its antioxidant activity was able to improve the DZN-induced ovarian toxicity.