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Abedi, H., Zarrin-Mehr, A., Ebrahimi, B., Haghshenas, H., Parvin, N., & Jahromi, H. K. (2024). The effect of aqueous extract of orchid root on the structure of ovary and hypothalamicpituitary- gonadal hormones in polycystic ovary syndrome rat model: An experimental study. International Journal of Reproductive BioMedicine (IJRM), 22(3), 203–210. https://doi.org/10.18502/ijrm.v22i3.16164
https://doi.org/10.18502/ijrm.v22i3.16164
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Hassanali Abedi
Hassanali Abedi
Research Center for Noncommunicable Disease, Jahrom University of Medical Sciences, Jahrom, Iran.
-
Armin Zarrin-Mehr
Armin Zarrin-Mehr
Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran.
-
Bahareh Ebrahimi
Bahareh Ebrahimi
Shiraz Geriatric Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
-
Hoda Haghshenas
Hoda Haghshenas
Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran.
-
Negar Parvin
Negar Parvin
Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran.
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Hossein Kargar Jahromi
Hossein Kargar Jahromi
Research Center for Noncommunicable Disease, Jahrom University of Medical Sciences, Jahrom, Iran.
Published Date
- May 12, 2024
The effect of aqueous extract of orchid root on the structure of ovary and hypothalamicpituitary- gonadal hormones in polycystic ovary syndrome rat model: An experimental study
Abstract
Background: Some medical conditions, including polycystic ovarian syndrome (PCOs), may lead to infertility. In PCOs, hormonal imbalance is significant. Antioxidants such as natural antioxidants have many health benefits, including positive effects on hormone production.
Objective: Since herbal medicines are more acceptable to people, the present study was designed to evaluate the effect of an aqueous extract of orchid (SA), with antioxidative effects, on the structure of the ovary and the hypothalamic-pituitarygonadal axis hormones and free testosterone in PCOs rats.
Materials and Methods: In this experimental study, 64 healthy female Wistar rats (180–200 g) were randomly divided into 60 and 89 day control groups, PCOs, and 4 PCOs + SA groups that received 40, 80, 160, and 320 mg/kg of SA. Serum levels of gonadotropin-releasing hormone, estrogen, progesterone, testosterone, follicle-stimulating hormone, and luteinizing hormone were measured. In addition, the ovaries were extracted and examined histologically.
Results: The amount of primordial, primary, secondary, and Graafian follicles and serum levels of Follicle-stimulating hormone and progesterone hormones decreased in PCOs groups, while atretic follicles and the serum levels of gonadotropin-releasing hormone, luteinizing hormone, estrogen, and free testosterone were increased. SA at different doses regulated hormonal and histological imbalances caused by PCOs, and 320 mg/kg was the most effective.
Conclusion: The aqueous extract of orchids root can have a positive effect on the improvement of polycystic ovary syndrome. This effect can be achieved by regulating the level of sex hormones and correcting follicular abnormalities in the ovarian tissue.
Key words: PCOS, Orchid, Ovary, GnRH, LH, FSH.
References
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[14] Abu Ahmed AM, Rahman A, Hossen A, Ali Reza ASM, Islam Sh, Rashid M, et al. Epiphytic acampe ochracea orchid relieves paracetamol-induced hepatotoxicity by inhibiting oxidative stress and upregulating antioxidant genes in in vivo and virtual screening. Biomed Pharmacother 2021; 143: 112215.
[15] Barragán-Zarate GS, Lagunez-Rivera L, Solano R, Pineda- Peña EA, Landa-Juárez AY, Chávez-Piña AE, et al. Prosthechea karwinskii, an orchid used as traditional medicine, exerts anti-inflammatory activity and inhibits ROS. J Ethnopharmacol 2020; 253: 112632.
[16] Amini L, Tehranian N, Movahedin M, Ramezani Tehrani F, Soltanghoraee H. Polycystic ovary morphology (PCOM) in estradiol valerate treated mouse model. Int J Women’s Health Reprod Sci 2016; 4: 13–17.
[17] Venegas B, De León Gordillo LY, Rosas G, Espinoza JA, Morán C, Domínguez R, et al. In rats with estradiol valerateinduced polycystic ovary syndrome, the acute blockade of ovarian ??-adrenoreceptors improve ovulation. Reprod Biol Endocrinol 2019; 17: 95.
[18] Atashpour Sh, Abedi H, Shafiei Jahromi N, Bagherzadeh MA, Saremi J, Mahjour A, et al. The effect of Salep Aqueous extract high doses on serum level of urea nitrogen, creatinine, uric acid and kidney histopathological changes in adult male wistar rats. Arch Razi Inst 2023; 78: 1451–1461.
[19] Kargar Jahromi H, Pourahmad M, Abedi HA, Karimi M, Kargar Jahromi Z. Protective effects of salep against isoniazid liver toxicity in wistar rats. J Tradit Complement Med 2018; 8: 239–243.
[20] Kargar Jahromi H, Khorram A, Atashpoor Sh, Jalali M, Kargar Jahromi Z, Mohammadi F, et al. The effect of hydroalcoholic extract of Salep on pentylenetetrazole- Iinduced seizure in adult male rats. Iran J Neurosurg 2023; 9: 1–9.
[21] Chauhan NS, Sharma V, Dixit VK, Thakur M. A review on plants used for improvement of sexual performance and virility. Biomed Res Int 2014; 2014: 868062.
[22] Lakshmi JN, Babu AN, Kiran SS, Nori LP, Hassan N, Ashames A, et al. Herbs as a source for the treatment of polycystic ovarian syndrome: A systematic review. BioTech 2023; 12: 4.
[23] Shahzad H, Giribabu N, Muniandy S, Salleh N. Quercetin induces morphological and proliferative changes of rat’s uteri under estrogen and progesterone influences. Int J Clin Exp Pathol 2014; 7: 5484.
[24] Han B, Jiang P, Jiang L, Li X, Ye X. Three phytosterols from sweet potato inhibit MCF7-xenograft-tumor growth through modulating gut microbiota homeostasis and SCFAs secretion. Food Res Int 2021; 141: 110147.
[25] Matsuda F, Ohkura S, Magata F, Munetomo A, Chen J, Sato M, et al. Role of kisspeptin neurons as a GnRH surge generator: Comparative aspects in rodents and nonrodent mammals. J Obstet Gynaecol Res 2019; 45: 2318– 2329.
[26] Watanabe M, Fukuda A, Nabekura J. The role of GABA in the regulation of GnRH neurons. Front Neurosci 2014; 8: 387.
[27] Hajiani M, Kargar Jahromi H, Kargar Jahromi Z, Khabbaz Kherameh Z. [Study on the effect of 4-weeks treatment of the aqueous extract of the roots of Salep plants on the serum concentration of leptin hormone and body weight in male rats]. J Jahrom Univ Med Sci 2014; 12: 9–14. (in Persian)
[28] Childs GV, Odle AK, MacNicol MC, MacNicol AM. The importance of leptin to reproduction. Endocrinology 2021; 162: bqaa204.
[29] Afradiasbagharani P, Hosseini E, Allahveisi A, Bazrafkan M. The insulin-like growth factor and its players: Their functions, significance, and consequences in all aspects of ovarian physiology. Middle East Fertil Soc J 2022; 27: 27.
[30] Li H, Yu X, Meng F, Zhao Z, Guan S, Wang L. Ferulic acid supplementation increases lifespan and stress resistance via insulin/IGF-1 signaling pathway in C. elegans. Int J Mol Sci 2021; 22: 4279.
[31] Sgrò P, Ceci R, Lista M, Patrizio F, Sabatini S, Felici F, et al. Quercetin modulates IGF-I and IGF-II levels after eccentric exercise-induced muscle-damage: A placebo-controlled study. Front Endocrinol 2021; 12:745959.
[32] Jahan S, Abid A, Khalid S, Afsar T, Shaheen G, Almajwal A, et al. Therapeutic potentials of quercetin in management of polycystic ovarian syndrome using letrozole induced rat model: A histological and a biochemical study. J Ovarian Res 2018; 11: 26.
[33] Du J, Ruan X, Jin F, Li Y, Cheng J, Gu M, et al. Abnormalities of early folliculogenesis and serum anti- Müllerian hormone in chinese patients with polycystic ovary syndrome. J Ovarian Res 2021; 14: 36.
[2] Guan C, Zahid S, Minhas AS, Ouyang P, Vaught A, Baker VL, et al. Polycystic ovary syndrome: A “risk-enhancing” factor for cardiovascular disease. Fertil Steril 2022; 117: 924–935.
[3] Pfieffer ML. Polycystic ovary syndrome: An update. Nursing 2019; 49: 34–40.
[4] Xu Y, Qiao J. Association of insulin resistance and elevated androgen levels with polycystic ovarian syndrome (PCOS): A review of literature. J Healthc Eng 2022; 2022: 9240569.
[5] Al Wattar BH, Fisher M, Bevington L, Talaulikar V, Davies M, Conway G, et al. Clinical practice guidelines on the diagnosis and management of polycystic ovary syndrome: A systematic review and quality assessment study. J Clin Endocrinol Metab 2021; 106: 2436–2446.
[6] Moran LJ, Tassone EC, Boyle J, Brennan L, Harrison CL, Hirschberg AL, et al. Evidence summaries and recommendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome: Lifestyle management. Obes Rev 2020; 21: e13046.
[7] Khashchenko E, Vysokikh M, Uvarova E, Krechetova L, Vtorushina V, Ivanets T, et al. Activation of systemic inflammation and oxidative stress in adolescent girls with polycystic ovary syndrome in combination with metabolic disorders and excessive body weight. J Clin Med 2020; 9: 1399.
[8] Enechukwu CI, Onuegbu AJ, Olisekodiaka MJ, Eleje GU, Ikechebelu JI, Ugboaja JO, et al. Oxidative stress markers and lipid profiles of patients with polycystic ovary syndrome in a Nigerian tertiary hospital. Obstet Gynecol Sci 2019; 62: 335–343.
[9] Kargar Jahromi H, Solhjoo E, Hassannezhad A, Pourahmadi M, Sahraei R, Abedi H, et al. [Comparison of aqueous extract of Salepa?s root (Dactylorhiza maculate) with ibuprofen on pain sensory thresholds using the formalin test in adult male rats]. Pars J Med Sci 2022; 18: 15–22. (in Persian)
[10] Easley T, Horne S. The modern herbal dispensatory: A medicine-making guide. US: North Atlantic Books; 2016.
[11] Sadeghzadeh F, Sadeghzadeh A, Changizi-Ashtiyani S, Alimoradian A, Mashayekhei M, Zarei A, et al. The effects of hydro-alcoholic extracts of Allium sativum L. and Orchismaculata L. on spermatogenesis index and testosterone level in cyclophosphamide-treated rats. J Kerman Univ Med Sci 2020; 27: 232–243.
[12] Guadarrama-Bazante IL, Rodríguez-Manzo G. Nucleus accumbens dopamine increases sexual motivation in sexually satiated male rats. Psychopharmacology 2019; 236: 1303–1312.
[13] Solhjo K, Kargar Jahromi H, Solhjo KA, Khabbaz Kherameh Z, Mahdiyar M. The effect of the aqueous extract of orchid roots on the serum concentration of progesterone and luteinizing hormone in adult female rats. Pars J Med Sci 2015; 13: 21–26.
[14] Abu Ahmed AM, Rahman A, Hossen A, Ali Reza ASM, Islam Sh, Rashid M, et al. Epiphytic acampe ochracea orchid relieves paracetamol-induced hepatotoxicity by inhibiting oxidative stress and upregulating antioxidant genes in in vivo and virtual screening. Biomed Pharmacother 2021; 143: 112215.
[15] Barragán-Zarate GS, Lagunez-Rivera L, Solano R, Pineda- Peña EA, Landa-Juárez AY, Chávez-Piña AE, et al. Prosthechea karwinskii, an orchid used as traditional medicine, exerts anti-inflammatory activity and inhibits ROS. J Ethnopharmacol 2020; 253: 112632.
[16] Amini L, Tehranian N, Movahedin M, Ramezani Tehrani F, Soltanghoraee H. Polycystic ovary morphology (PCOM) in estradiol valerate treated mouse model. Int J Women’s Health Reprod Sci 2016; 4: 13–17.
[17] Venegas B, De León Gordillo LY, Rosas G, Espinoza JA, Morán C, Domínguez R, et al. In rats with estradiol valerateinduced polycystic ovary syndrome, the acute blockade of ovarian ??-adrenoreceptors improve ovulation. Reprod Biol Endocrinol 2019; 17: 95.
[18] Atashpour Sh, Abedi H, Shafiei Jahromi N, Bagherzadeh MA, Saremi J, Mahjour A, et al. The effect of Salep Aqueous extract high doses on serum level of urea nitrogen, creatinine, uric acid and kidney histopathological changes in adult male wistar rats. Arch Razi Inst 2023; 78: 1451–1461.
[19] Kargar Jahromi H, Pourahmad M, Abedi HA, Karimi M, Kargar Jahromi Z. Protective effects of salep against isoniazid liver toxicity in wistar rats. J Tradit Complement Med 2018; 8: 239–243.
[20] Kargar Jahromi H, Khorram A, Atashpoor Sh, Jalali M, Kargar Jahromi Z, Mohammadi F, et al. The effect of hydroalcoholic extract of Salep on pentylenetetrazole- Iinduced seizure in adult male rats. Iran J Neurosurg 2023; 9: 1–9.
[21] Chauhan NS, Sharma V, Dixit VK, Thakur M. A review on plants used for improvement of sexual performance and virility. Biomed Res Int 2014; 2014: 868062.
[22] Lakshmi JN, Babu AN, Kiran SS, Nori LP, Hassan N, Ashames A, et al. Herbs as a source for the treatment of polycystic ovarian syndrome: A systematic review. BioTech 2023; 12: 4.
[23] Shahzad H, Giribabu N, Muniandy S, Salleh N. Quercetin induces morphological and proliferative changes of rat’s uteri under estrogen and progesterone influences. Int J Clin Exp Pathol 2014; 7: 5484.
[24] Han B, Jiang P, Jiang L, Li X, Ye X. Three phytosterols from sweet potato inhibit MCF7-xenograft-tumor growth through modulating gut microbiota homeostasis and SCFAs secretion. Food Res Int 2021; 141: 110147.
[25] Matsuda F, Ohkura S, Magata F, Munetomo A, Chen J, Sato M, et al. Role of kisspeptin neurons as a GnRH surge generator: Comparative aspects in rodents and nonrodent mammals. J Obstet Gynaecol Res 2019; 45: 2318– 2329.
[26] Watanabe M, Fukuda A, Nabekura J. The role of GABA in the regulation of GnRH neurons. Front Neurosci 2014; 8: 387.
[27] Hajiani M, Kargar Jahromi H, Kargar Jahromi Z, Khabbaz Kherameh Z. [Study on the effect of 4-weeks treatment of the aqueous extract of the roots of Salep plants on the serum concentration of leptin hormone and body weight in male rats]. J Jahrom Univ Med Sci 2014; 12: 9–14. (in Persian)
[28] Childs GV, Odle AK, MacNicol MC, MacNicol AM. The importance of leptin to reproduction. Endocrinology 2021; 162: bqaa204.
[29] Afradiasbagharani P, Hosseini E, Allahveisi A, Bazrafkan M. The insulin-like growth factor and its players: Their functions, significance, and consequences in all aspects of ovarian physiology. Middle East Fertil Soc J 2022; 27: 27.
[30] Li H, Yu X, Meng F, Zhao Z, Guan S, Wang L. Ferulic acid supplementation increases lifespan and stress resistance via insulin/IGF-1 signaling pathway in C. elegans. Int J Mol Sci 2021; 22: 4279.
[31] Sgrò P, Ceci R, Lista M, Patrizio F, Sabatini S, Felici F, et al. Quercetin modulates IGF-I and IGF-II levels after eccentric exercise-induced muscle-damage: A placebo-controlled study. Front Endocrinol 2021; 12:745959.
[32] Jahan S, Abid A, Khalid S, Afsar T, Shaheen G, Almajwal A, et al. Therapeutic potentials of quercetin in management of polycystic ovarian syndrome using letrozole induced rat model: A histological and a biochemical study. J Ovarian Res 2018; 11: 26.
[33] Du J, Ruan X, Jin F, Li Y, Cheng J, Gu M, et al. Abnormalities of early folliculogenesis and serum anti- Müllerian hormone in chinese patients with polycystic ovary syndrome. J Ovarian Res 2021; 14: 36.