https://doi.org/10.18502/ijrm.v21i10.14540
statistics
- 73 Abstract Views
- 44 PDF Views
- 0 HTML Views
authors
-
Anahita Abbasifard
Anahita Abbasifard
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
-
Shohreh Alimohammadi
Shohreh Alimohammadi
Department of Obstetrics and Gynecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
-
Ebrahim Abbasi Oshaghi
Ebrahim Abbasi Oshaghi
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
-
Gholamreza Shafiee
Gholamreza Shafiee
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Published Date
- Nov 21, 2023
Association of proprotein convertase subtilisin/kexin type 9 protein and oxidative stress indicators in women with preeclampsia: A case-control study
Abstract
Background: Proprotein convertase subtilisin/kexin type 9 (PCSK9) protein is one of the factors associated with oxidative stress and dyslipidemia disorders.
Objective: This study aimed to evaluate the lipid profile, PCSK9 levels, and oxidative stress in preeclampsia.
Materials and Methods: This case-control study was conducted at Sina hospital in Hamadan University of Medical Sciences, Hamadan, Iran from August 2020-May 2021. The average maternal age of included participants was 30 yr with 30 preeclampsia and 30 healthy pregnant women. After clinical examination, the fasting blood samples were collected, and the serum PCSK9 protein concentration, superoxide dismutase, glutathione peroxidase activities, and glutathione levels were determined by enzyme-linked immunosorbent assay. Total antioxidant capacity, total oxidant status, and malondialdehyde levels were determined manually.
Results: The average maternal age of participants were 29.97 ± 4.75 and 31.23 ± 5.85 yr, respectively. The concentrations of total cholesterol, low-density lipoprotein cholesterol (LDL-C), PCSK9, total antioxidant capacity, and malondialdehyde levels were higher in the preeclampsia group compared with control (p < 0.02). Total oxidant status, glutathione levels, superoxide dismutase, glutathione peroxidase activities were lower in the cases group compared with the control group (p < 0.01). The PCSK9 variable had a significant negative association with antioxidant parameters; however, a significant positive association was observed between PCSK9 level and parameters of LDL-C.
Conclusion: PCSK9 is associated with increased serum levels of LDL-C and oxidative factors in pregnant women that increase the risk of endothelial damage and hypertension in preeclampsia.
Key words: PCSK9, Hypercholesterolemia, Oxidative stress, Preeclampsia.
References
[2] Kirollos S, Skilton M, Patel S, Arnott C. A systematic review of vascular structure and function in pre-eclampsia: Non-invasive assessment and mechanistic links. Front Cardiovasc Med 2019; 6: 166.
[3] Mazloomi S, Khodadadi I, Alimohammadi Sh, Shafiee Gh. Correlation of thioredoxin reductase (TrxR) and nitric oxide synthase (NOS) activities with serum trace elements in preeclampsia. Clin Exp Hypertens 2021; 43: 120–124.
[4] Bell AS, Wagner J, Rosoff DB, Lohoff FW. Proprotein convertase subtilisin/kexin type 9 (PCSK9) in the central nervous system. Neurosci Biobehav Rev 2023; 149: 105155.
[5] Tummala LS, Agrawal A, Lundberg G. Management considerations for lipid disorders during pregnancy. Curr Treat Options Cardio Med 2021; 23: 346–358.
[6] Chao TH, Chen IC, Li YH, Lee PT, Tseng SY. Plasma levels of proprotein convertase subtilisin/kexin type 9 are elevated in patients with peripheral artery disease and associated with metabolic disorders and dysfunction in circulating progenitor cells. J Am Heart Assoc 2016; 5: e003497.
[7] Asadi S, Goodarzi MT, Karimi J, Hashemnia M, Khodadadi I. Does curcumin or metformin attenuate oxidative stress and diabetic nephropathy in rats? J Nephropathol 2019; 8: 1–9.
[8] Mazloomi S, Alimohammadi Sh, Khodadadi I, Ghiasvand T, Shafiee Gh. Evaluation of methylene tetrahydrofolate reductase (MTHFR) activity and the levels of homocysteine and malondialdehyde (MDA) in the serum of women with preeclampsia. Clin Exp Hypertens 2020; 42: 590–594.
[9] Mazloomi S, Khodadadi I, Alizadeh N, Shafiee Gh. Association of glutamate cystein ligase (GCL) activity peroxiredoxin 4 (prxR4) and apelin levels in women with preeclampsia. Pregnancy Hypertens 2021; 23: 163–168.
[10] Yurtseven E, Ura D, Baysa K, Tokgözoglu L. An update on the role of PCSK9 in atherosclerosis. J Atheroscler Thromb 2020; 27: 909–918.
[11] Cui C-J, Jin J-L, Guo L-N, Sun J, Wu N-Q, Guo Y-L, et al. Beneficial impact of epigallocatechingallate on LDLC through PCSK9/LDLR pathway by blocking HNF1a and activating FoxO3a. J Transl Med 2020; 18: 195.
[12] Habibitabar E, Khanverdiloo Sh, Doostizadeh M, Jahangard L, Karimi J, Shafiee Gh. The PCSK9 protein is not necessarily a risk factor for MDD. Basic Clin Neurosci 2022. (In Press)
[13] Cui C-J, Li S, Zhu C-G, Sun J, Du Y, Zhanget Y, et al. Enhanced pro-protein convertase subtilisin/kexin type 9 expression by C-reactive protein through p38 MAPK-HNF 1a pathway in HepG2 cells. J Cell Mol Med 2016; 20: 2374– 2383.
[14] Schluter K, Wolf A, Weber M, Schreckenberg R, Schulz R. Oxidized low-density lipoprotein (oxLDL) affects loadfree cell shortening of cardiomyocytes in a proprotein convertase subtilisin/kexin 9 (PCSK9)-dependent way. Basic Res Cardiol 2017; 112: 63.
[15] Shapiro MD, Fazio S. PCSK9 and atherosclerosis-lipids and beyond. J Atheroscler Thromb 2017; 24: 462–472.
[16] Pecks U, Rath W, Maass N, Berger B, Lueg I, Farrokh A, et al. Fetal gender and gestational age differentially affect PCSK9 levels in intrauterine growth restriction. Lipids Health Dis 2016; 15: 193.
[17] Silbernagel G, Steiner LK, Hollstein T, Fauler G, Scharnagl H, Stojakovic T, et al. The interrelations between PCSK9 metabolism and cholesterol synthesis and absorption. J Lipid Res 2019; 60: 161–167.
[18] Hor SL, Teoh SL, Lim WL. Plant polyphenols as neuroprotective agents in Parkinson’s disease targeting oxidative stress. Current Drug Targets 2020; 21: 458–476.
[19] Negre-Salvayre A, Swiader A, Salvayre R, Guerby P. Oxidative stress, lipid peroxidation and premature placental senescence in preeclampsia. Arch Biochem Biophys 2022; 730: 109416.